FastQCFastQC Report
Fri 10 Feb 2017
ERR1633428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647077
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33390.5160127774592513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.374298576521805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14050.2171302642498497No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11030.1704588480196329No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA9270.14325961207089727No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9100.14063241314403077No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8730.13491439195026247No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8640.13352352193015668No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7840.1211602328625496No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7010.10833332045490722No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6680.10323346371451929No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6670.1030789226011742No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6490.10029718256096261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG358.86519E-426.4285716
GGTATCA5700.024.991231
GTATCAA16650.024.4444451
CGTCTCC400.001930280423.12510
CGTTATT400.001930280423.12510
CCGGTTC652.679837E-622.7692321
GGTTCGC652.679837E-622.7692323
CGGTTCG753.7355494E-722.19999922
CATAATA2300.020.9130442
TTCTGCG951.6732884E-719.47368418
TTAACGG2300.019.30434835
GCACCGC2350.018.89361810
TCTAGTA500.007031639618.52
TGCGGGT902.1492597E-618.521
AACGGCC2500.018.537
GGACCGT609.230869E-418.56
ACGGTAC609.230869E-418.53
CACCGCC2450.018.12244811
TAACGGC2600.017.78846236
GCGGGGT1156.392111E-817.69565229
Produced by FastQC (version 0.11.5)