FastQCFastQC Report
Fri 10 Feb 2017
ERR1633427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580712
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29360.5055862458499222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20740.3571477772114232No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13190.22713496535287717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11650.20061579578173No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9300.1601482318257587No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8770.1510215046356886No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8500.1463720398407472No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8130.14000055104767942No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7320.12605215666285527No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6490.11175935747840583No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6460.11124275027896788No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6400.11020953588009201No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6160.10607667828458857No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5910.10177161828927248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG250.005494131729.69
ATCGGGC250.005494131729.625
GGTATCA6000.025.91
GTATCAA14800.021.6251
TTAACGG2150.019.79069735
ATCAAGC4350.019.13793230
ATTAGAG2550.018.8627453
CCGGAAT500.007030793518.53
GTCTAAT500.007030793518.51
TATGCCG500.007030793518.55
GCTTCGC500.007030793518.522
ATAAGAC500.007030793518.53
CCGGTCG609.229316E-418.59
CGGTCGT500.007030793518.510
GCATCAG4500.018.08888815
AGGCCAT4650.017.90322525
TATTAGA2600.017.7884622
TACATGC2400.017.7291662
AACGGCC2400.017.72916637
CAAGCAG4800.017.72916632
Produced by FastQC (version 0.11.5)