FastQCFastQC Report
Fri 10 Feb 2017
ERR1633426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780580
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52110.6675805170514233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.47618437572061795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19650.2517358886981475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.11542699018678418No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8020.10274411335161034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT502.718898E-729.59999828
GTATCAA22900.023.589521
GGACGTA551.9016441E-523.54545627
GGTATCA8550.023.3684221
TCGGCGT400.001930634723.12500211
GTCGTGT400.001930634723.12500212
CCGGTCG400.001930634723.1250029
GCCCGAA400.001930634723.12500227
ACGTATA603.724314E-521.58333429
GCGGTCC450.003824724820.55555520
CCGACGA450.003824724820.55555513
TTAACGG1500.019.73333235
GCCGTTA1153.0449883E-919.30434815
GACCGTT801.6155873E-518.57
TAGGACC2050.018.0487824
AACGGCC1653.6379788E-1217.93939437
GTCAACC1552.0008883E-1117.90322715
CTTAGGC1258.565621E-917.763
AGGTCGC1258.565621E-917.7635
GGACCGT1054.792382E-717.6190476
Produced by FastQC (version 0.11.5)