FastQCFastQC Report
Fri 10 Feb 2017
ERR1633425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537202
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23250.4327980908485077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.32557585414797413No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10510.19564335203517488No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9960.18540511762800585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9900.18428821932904196No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9440.17572533237031882No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8500.15822725901988452No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8340.1552488635559808No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7630.1420322336849081No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7100.13216629871072708No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6470.12043886657160621No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6460.12025271685511224No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6360.11839121969017241No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6170.11485437507678675No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6030.11224827904587102No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6020.11206212932937704No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6010.11187597961288306No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5940.11057293159742518No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5800.10796683556650943No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5710.10629148811806359No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5650.1051745898190997No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5480.10201004463870202No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5440.10126544577272609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.028.6666661
CACCGTA358.8632235E-426.4285731
CCGAGCT400.00192985623.12523
GACGATT400.00192985623.12514
GCCGTTA1650.022.42424215
GTATCAA14150.021.4416961
GGCCGTT1650.021.3030314
GGTACCG1750.021.1428576
CCGTTAA1750.021.14285716
TATACTG1151.3460522E-1020.9130445
TCACCGT450.003823195820.55555530
CGGCCGT1750.020.08571413
CGCGGCC1800.019.52777711
GGGTACC2050.018.9512185
GTTAAAC2100.018.518
GGTTATA801.6140602E-518.51
TGCGAAG500.00703012518.49999823
GTGTCAC2000.018.49999827
TAGGACC1700.018.4999984
AGGGTAC2050.018.048784
Produced by FastQC (version 0.11.5)