FastQCFastQC Report
Fri 10 Feb 2017
ERR1633424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675325
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30640.4537074741790989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.32399215192685005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12320.1824306815237108No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9280.13741531855032763No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8660.12823455373338763No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7820.1157960981749528No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7520.11135379261836893No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7090.10498648798726538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT405.936555E-527.7500026
GGTATCA5350.026.9719621
ACCGTTA751.371518E-824.6666668
GTATCAA14750.022.8271181
TCGTTGG450.00382419920.55555522
TTAACGG2700.019.87036935
CCGTTAT951.6735612E-719.4736849
TAACGGC2750.018.83636536
GCACCGC2750.018.83636510
GAGTCGT609.2314405E-418.519
GTATTAG4350.018.2873551
CACCGCC2850.018.17543811
AACGGCC2900.017.86206837
AACCGTT650.001579021917.0769237
CTATGGT650.001579021917.0769234
TGGGGCG1251.6546255E-716.27999937
CGCGGCT803.3799803E-416.187520
AATGGAC803.3799803E-416.18753
CGTTATA803.3799803E-416.187510
GCTCTAG803.3799803E-416.18751
Produced by FastQC (version 0.11.5)