FastQCFastQC Report
Fri 10 Feb 2017
ERR1633423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940770
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60790.6461728158848603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.44378540982386766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24300.2582990529034727No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA20070.2133358844350904No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG18610.19781668207957312No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG18240.19388373353742147No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG16130.17145529725650266No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14870.15806201303187814No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14610.15529831946171752No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT13180.14009800482583415No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11830.1257480574423079No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC11640.12372843521795975No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG11390.12107103755434377No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC11350.12064585392816522No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT11150.11851993579727245No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA11150.11851993579727245No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG11110.11809475217109389No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11090.11788216035800463No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11040.11735068082528143No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC10780.1145869872551208No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG10170.10810293695589783No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT10000.10629590654463897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.027.8623471
GTATCAA32300.023.1393181
CGCTCTC1001.2891178E-820.34999829
GCTATAC555.1421404E-420.1818183
GTACTAT801.6161623E-518.51
TATACTG902.1513715E-618.55
GTCGCCC1552.0008883E-1117.90322737
GCCGGCA1156.4012056E-817.6956515
TTCGCCG1054.794965E-717.61904724
CGGGTCC752.0667643E-417.26666616
TTCGGTC650.001579645417.07692328
CCGCTCT1107.803901E-716.81818228
CCGTTTA1452.9758667E-916.58620627
TAGTCTG904.444576E-516.4444455
AGGTCGC1801.0913936E-1116.44444535
TTAACGG1708.54925E-1116.3235335
CGGTCAC803.3817493E-416.187530
ATTAGAG2300.016.0869563
CGAGTCG1151.241533E-616.08695621
CTATGAC700.00259202415.8571433
Produced by FastQC (version 0.11.5)