FastQCFastQC Report
Fri 10 Feb 2017
ERR1633422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981140
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56200.5728030658213914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.3974967894490083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22790.23228081619340768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.023.4154591
AACGTGA400.001930986823.12510
GTATCAA27950.023.1001781
TTCGCCG1005.347829E-1022.224
CCGTCGT450.003825415420.55555511
CAGTCCG951.6754893E-719.4736849
CCGCCGT951.6754893E-719.47368424
GTTACGG500.007034165318.517
CCGACAA609.2355034E-418.533
TAGTACT902.1515625E-618.54
CCGTTAA852.722458E-517.41176416
ATCTCGT752.0668775E-417.26666637
CCGAGTT650.001579711617.07692113
TAGAACC1005.876025E-616.6500024
CGCCGTC1352.2178938E-816.44444526
GTCCGAC803.3819338E-416.187511
ACACGAT700.002592130315.85714334
CGAGTCG1403.4708137E-815.85714321
GACCGTT700.002592130315.8571437
GCCGCTC1403.4708137E-815.85714327
Produced by FastQC (version 0.11.5)