##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633422.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	981140
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87324540840247	33.0	33.0	33.0	33.0	33.0
2	31.98479421896977	33.0	33.0	33.0	33.0	33.0
3	31.697228733921765	33.0	33.0	33.0	27.0	33.0
4	31.984076686303688	33.0	33.0	33.0	33.0	33.0
5	32.08172941680086	33.0	33.0	33.0	33.0	33.0
6	35.24073424791569	37.0	37.0	37.0	33.0	37.0
7	35.386097804594655	37.0	37.0	37.0	33.0	37.0
8	35.53233891187802	37.0	37.0	37.0	33.0	37.0
9	35.64410889373586	37.0	37.0	37.0	33.0	37.0
10	35.647167580569544	37.0	37.0	37.0	33.0	37.0
11	35.67446745622439	37.0	37.0	37.0	33.0	37.0
12	35.67126302056791	37.0	37.0	37.0	33.0	37.0
13	35.66163748292802	37.0	37.0	37.0	33.0	37.0
14	35.64480094583851	37.0	37.0	37.0	33.0	37.0
15	35.67042114275231	37.0	37.0	37.0	33.0	37.0
16	35.647984997044254	37.0	37.0	37.0	33.0	37.0
17	35.64560715086532	37.0	37.0	37.0	33.0	37.0
18	35.622873392176444	37.0	37.0	37.0	33.0	37.0
19	35.6178761440773	37.0	37.0	37.0	33.0	37.0
20	35.62250035672789	37.0	37.0	37.0	33.0	37.0
21	35.61011782212528	37.0	37.0	37.0	33.0	37.0
22	35.51958028415925	37.0	37.0	37.0	33.0	37.0
23	35.568428562692375	37.0	37.0	37.0	33.0	37.0
24	35.56212467130073	37.0	37.0	37.0	33.0	37.0
25	35.58167234033879	37.0	37.0	37.0	33.0	37.0
26	35.472290396885256	37.0	37.0	37.0	33.0	37.0
27	35.49290213425199	37.0	37.0	37.0	33.0	37.0
28	35.50702855861549	37.0	37.0	37.0	33.0	37.0
29	35.51486739914793	37.0	37.0	37.0	33.0	37.0
30	35.518612022749046	37.0	37.0	37.0	33.0	37.0
31	35.51029210917912	37.0	37.0	37.0	33.0	37.0
32	35.498685202927206	37.0	37.0	37.0	33.0	37.0
33	35.48883543632917	37.0	37.0	37.0	33.0	37.0
34	35.4695130154718	37.0	37.0	37.0	33.0	37.0
35	35.40987320871639	37.0	37.0	37.0	33.0	37.0
36	35.428900055038014	37.0	37.0	37.0	33.0	37.0
37	35.43224820107222	37.0	37.0	37.0	33.0	37.0
38	35.41991255070632	37.0	37.0	37.0	33.0	37.0
39	35.320283547709806	37.0	37.0	37.0	33.0	37.0
40	35.10208329086573	37.0	37.0	37.0	33.0	37.0
41	35.26781397150254	37.0	37.0	37.0	33.0	37.0
42	35.31942128544346	37.0	37.0	37.0	33.0	37.0
43	34.98183643516725	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	26.0
16	61.0
17	58.0
18	35.0
19	32.0
20	68.0
21	147.0
22	381.0
23	1047.0
24	1993.0
25	3810.0
26	6227.0
27	9503.0
28	13389.0
29	18107.0
30	23631.0
31	30311.0
32	38726.0
33	50963.0
34	73892.0
35	151246.0
36	557485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31021057137615	20.789489777197954	14.194100739955562	23.70619891147033
2	15.560164706361986	22.307315979370937	39.980023238273844	22.152496075993234
3	17.740179790855535	27.73498175591659	30.985690115579835	23.53914833764804
4	12.29671606498563	18.15062070652506	40.210265609393154	29.342397619096154
5	13.327965427971545	37.6370344701062	35.37456428236541	13.660435819556843
6	28.21248751452392	40.677273375868886	17.71745112827935	13.392787981327844
7	25.436329168110568	33.07326171596306	23.881403265589007	17.60900585033736
8	24.000754224677415	36.189942312004405	21.107283364249753	18.70202009906843
9	25.895183154289906	15.894367776260268	20.556903194243432	37.65354587520639
10	14.576207269095134	28.64932629390301	34.10736490205271	22.66710153494914
11	32.87267872067187	24.497013677966446	24.524532686466763	18.10577491489492
12	22.659355443667568	26.26454940171637	30.870721813400735	20.20537334121532
13	27.98917585665654	22.827221395519498	26.833785188658094	22.349817559165867
14	21.938357420959292	21.869559899708502	28.51224086266996	27.679841816662247
15	23.40991091995026	29.093299631041443	25.36641050206902	22.130378946939274
16	22.095521536172207	29.33628228387386	25.621216136331203	22.946980043622727
17	21.749087795829343	28.030862058421835	27.050268055527244	23.16978209022158
18	22.74232015818334	27.237805002344214	28.42662616955786	21.593248669914587
19	24.490286809221924	26.458099761501924	28.64973398291783	20.40187944635832
20	24.214994801965062	26.29604337811118	28.449456754387754	21.03950506553601
21	22.585054120716716	27.390484538394112	28.074179016246408	21.95028232464276
22	22.057606457793995	27.38875186008113	28.24214689035204	22.311494791772834
23	22.073710173879366	27.235766557270114	28.662372342377235	22.028150926473288
24	22.482520333489614	27.42850153902603	27.98397782171759	22.10500030576676
25	22.487514523921153	27.26012597590558	28.507450516745823	21.74490898342744
26	22.580263774792588	27.171249770674926	28.235929632876044	22.01255682165644
27	22.49434331491938	27.179097784210203	27.997431559206635	22.329127341663778
28	21.902276943147765	27.378763479218055	28.520190798458934	22.198768779175246
29	22.737631734512913	27.296817987239336	27.965835660558124	21.999714617689627
30	22.425647715922295	27.326579285321156	28.416841633202196	21.830931365554353
31	22.6662861569195	27.088794667427685	28.219723994536967	22.025195181115844
32	21.616996555027825	27.077685141773856	28.588988319709724	22.716329983488595
33	21.8297082985099	26.92225370487392	29.04060582587602	22.20743217074016
34	22.075952463460872	26.90778074484783	28.441201051837655	22.57506573985364
35	22.174715127300896	27.668630368754716	28.238477689218666	21.918176814725726
36	22.526244980329004	27.279185437348392	28.141651548199032	22.05291803412357
37	22.82243104959537	26.862527264202868	27.889903581547998	22.42513810465377
38	22.660680432965734	27.286625761868848	27.99141814623805	22.06127565892737
39	22.282446949466948	26.946001589987155	28.33632305277534	22.435228407770552
40	22.261349042950037	27.36602319750494	28.362517071977493	22.010110687567526
41	22.21894938540881	26.767943412764744	28.536090670036895	22.477016531789552
42	22.079213975579428	27.166765191511917	28.289744582832217	22.46427625007644
43	22.23250504515156	26.8024950567707	28.28413885887845	22.6808610391993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	62.5
2	84.0
3	318.5
4	553.0
5	553.0
6	644.0
7	735.0
8	722.0
9	709.0
10	995.0
11	1281.0
12	1281.0
13	2013.5
14	2746.0
15	5200.0
16	7654.0
17	8903.5
18	10153.0
19	10153.0
20	9939.0
21	9725.0
22	10894.0
23	12063.0
24	15538.5
25	19014.0
26	19014.0
27	23022.5
28	27031.0
29	32736.5
30	38442.0
31	42054.5
32	45667.0
33	45667.0
34	50549.5
35	55432.0
36	58477.0
37	61522.0
38	64187.5
39	66853.0
40	66853.0
41	69461.0
42	72069.0
43	70550.5
44	69032.0
45	69445.5
46	69859.0
47	69859.0
48	68492.0
49	67125.0
50	67797.5
51	68470.0
52	67623.0
53	66776.0
54	66776.0
55	61162.5
56	55549.0
57	49733.0
58	43917.0
59	39007.5
60	34098.0
61	34098.0
62	29741.5
63	25385.0
64	21026.0
65	16667.0
66	14152.0
67	11637.0
68	11637.0
69	9599.0
70	7561.0
71	6534.5
72	5508.0
73	4407.5
74	3307.0
75	3307.0
76	2503.0
77	1699.0
78	1342.5
79	986.0
80	793.5
81	601.0
82	601.0
83	497.5
84	394.0
85	392.0
86	390.0
87	311.0
88	232.0
89	232.0
90	182.0
91	132.0
92	84.0
93	36.0
94	20.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	981140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.42899853076105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64894159553387	53.84186704494811
2	7.556180429189214	9.283371929656594
3	1.7356126584306426	3.198508423341187
4	0.7568874235796776	1.859793457241101
5	0.4225741057143314	1.2979152059531662
6	0.28978095330157505	1.0680572252762994
7	0.21492404333709075	0.9241798119665561
8	0.15815714606126452	0.7772348074421424
9	0.11300203623891798	0.624744172628415
>10	0.8954242730596473	11.237997753841968
>50	0.13515397761041997	5.877536375423094
>100	0.07084323360338811	7.99509331396694
>500	0.002014499471931822	0.7969415320537812
>1k	3.3574991198863705E-4	0.6372026043685707
>5k	1.6787495599431852E-4	0.5795563418921131
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5620	0.5728030658213914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3900	0.3974967894490083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2279	0.23228081619340768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	3.0576676111462175E-4	0.0	0.0
4	0.0	0.0	4.076890148194957E-4	1.0192225370487393E-4	0.0
5	0.0	0.0	4.076890148194957E-4	1.0192225370487393E-4	0.0
6	0.0	0.0	9.173002833438653E-4	1.0192225370487393E-4	0.0
7	0.0	0.0	0.0028538231037364696	1.0192225370487393E-4	0.0
8	1.0192225370487393E-4	0.0	0.0033634343722608396	1.0192225370487393E-4	0.0
9	1.0192225370487393E-4	0.0	0.004280734655604705	3.0576676111462175E-4	0.0
10	3.0576676111462175E-4	0.0	0.004790345924129074	3.0576676111462175E-4	0.0
11	3.0576676111462175E-4	0.0	0.007236480013046049	4.076890148194957E-4	0.0
12	3.0576676111462175E-4	0.0	0.008153780296389914	4.076890148194957E-4	0.0
13	3.0576676111462175E-4	0.0	0.009071080579733779	4.076890148194957E-4	0.0
14	3.0576676111462175E-4	0.0	0.009682614101963022	5.096112685243696E-4	0.0
15	3.0576676111462175E-4	0.0	0.010803758892716635	5.096112685243696E-4	0.0
16	3.0576676111462175E-4	0.0	0.011619136922355626	7.134557759341174E-4	0.0
17	3.0576676111462175E-4	0.0	0.011619136922355626	8.153780296389914E-4	0.0
18	3.0576676111462175E-4	0.0	0.012026825937175124	9.173002833438653E-4	0.0
19	3.0576676111462175E-4	0.0	0.012230670444584871	9.173002833438653E-4	0.0
20	3.0576676111462175E-4	0.0	0.012332592698289745	0.0010192225370487392	0.0
21	3.0576676111462175E-4	0.0	0.012944126220518989	0.0014269115518682348	0.0
22	4.076890148194957E-4	0.0	0.013046048474223863	0.0020384450740974784	0.0
23	4.076890148194957E-4	0.0	0.013046048474223863	0.002649978596326722	0.0
24	4.076890148194957E-4	0.0	0.013046048474223863	0.0035672788796705874	0.0
25	4.076890148194957E-4	0.0	0.013046048474223863	0.0036692011333754613	0.0
26	4.076890148194957E-4	0.0	0.013046048474223863	0.004280734655604705	0.0
27	4.076890148194957E-4	1.0192225370487393E-4	0.01324989298163361	0.005605723953768065	0.0
28	4.076890148194957E-4	1.0192225370487393E-4	0.01324989298163361	0.01426911551868235	0.0
29	4.076890148194957E-4	1.0192225370487393E-4	0.01324989298163361	0.03404203273742789	0.0
30	4.076890148194957E-4	1.0192225370487393E-4	0.01324989298163361	0.057280306582139145	0.0
31	4.076890148194957E-4	1.0192225370487393E-4	0.013351815235338484	0.11700674725319526	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1035	0.0	23.415459	1
AACGTGA	40	0.0019309868	23.125	10
GTATCAA	2795	0.0	23.100178	1
TTCGCCG	100	5.347829E-10	22.2	24
CCGTCGT	45	0.0038254154	20.555555	11
CAGTCCG	95	1.6754893E-7	19.473684	9
CCGCCGT	95	1.6754893E-7	19.473684	24
GTTACGG	50	0.0070341653	18.5	17
CCGACAA	60	9.2355034E-4	18.5	33
TAGTACT	90	2.1515625E-6	18.5	4
CCGTTAA	85	2.722458E-5	17.411764	16
ATCTCGT	75	2.0668775E-4	17.266666	37
CCGAGTT	65	0.0015797116	17.076921	13
TAGAACC	100	5.876025E-6	16.650002	4
CGCCGTC	135	2.2178938E-8	16.444445	26
GTCCGAC	80	3.3819338E-4	16.1875	11
ACACGAT	70	0.0025921303	15.857143	34
CGAGTCG	140	3.4708137E-8	15.857143	21
GACCGTT	70	0.0025921303	15.857143	7
GCCGCTC	140	3.4708137E-8	15.857143	27
>>END_MODULE