FastQCFastQC Report
Fri 10 Feb 2017
ERR1633421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842450
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198452.3556294142085585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146101.7342275505964746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80230.9523413852454152No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA36280.4306487031871328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33510.39776841355570064No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA33050.39230814885156384No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG31450.37331592379369694No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG28710.3407917383820998No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT27560.32714107662175795No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26030.3089797614101727No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC22520.2673155676894771No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.24535580746631847No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19400.2302807288266366No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG18280.21698617128612974No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA17930.21283162205472136No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17870.21211941361505135No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17530.20808356579025464No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC17300.20535343343818627No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC17140.20345421093239954No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16980.20155498842661285No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC16080.1908718618315627No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT15450.18339367321502759No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14730.1748471719389875No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14690.1743723663125408No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14560.1728292480265891No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14340.1702178170811324No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC14240.16903080301501572No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13730.16297703127782062No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13500.16024689892575228No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA13460.1597720932993056No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13350.15846637782657724No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13310.15799157220013058No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13010.15443053000178053No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12990.15419312718855718No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12950.15371832156211052No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12790.15181909905632382No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG12630.14991987655053712No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12420.1474271470116921No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12340.14647753575879874No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12320.1462401329455754No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12220.14505311887945874No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12200.1448157160662354No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11720.1391180485488753No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.1387619443290403No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11640.13816843729598194No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11410.13543830494391357No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11330.13448869369102023No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.1330642768116802No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11030.1309276514926702No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10940.12985933883316517No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10540.12511128256869844No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10300.12226244881001841No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10090.11976971927117337No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10090.11976971927117337No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9950.11810789957861No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9950.11810789957861No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9950.11810789957861No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9880.11727698973232834No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9820.11656478129265832No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9800.11632737847943497No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9790.11620867707282331No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9680.11490296160009497No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9630.11430945456703662No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.11312244050091992No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA9440.11205412784141493No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9370.11122321799513324No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9270.11003620392901657No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG9150.10861178704967653No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9110.10813698142322986No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA9090.10789957861000654No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9030.10718737017033653No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9000.10683126595050152No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9000.10683126595050152No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8960.10635646032405484No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG8870.10528814766454983No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG8780.10421983500504481No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8530.10125229983975309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC352.387557E-531.7142851
CTAGTGC850.030.4705899
TAGTGCA850.030.47058910
TGGGTGG250.00549552129.66
TGTGCAG250.00549552129.610
AAATTGT250.00549552129.66
TACAGCT250.00549552129.630
AGCTTCT702.0736479E-1029.07142837
AGCAACG454.006588E-628.7777772
AGCCCAC905.456968E-1226.72222117
TTGATGA358.867422E-426.428573
ATGGCGG358.867422E-426.4285732
CGTCCTA358.867422E-426.4285712
CGAGCCC951.0913936E-1125.3157915
ATCTCGT901.4188117E-1024.66666637
AAGAGCG451.3228026E-424.66666618
GTGCTGG451.3228026E-424.6666661
CTATTTC601.3366989E-624.66666616
GGTATCA37200.024.0698931
TCTCCGA1002.0008883E-1124.05000111
Produced by FastQC (version 0.11.5)