FastQCFastQC Report
Fri 10 Feb 2017
ERR1633420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019248
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59230.5811147041740578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.3901896300017268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23340.22899235514810917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.024.7767851
GTATCAA31300.023.93771
TAGACCG502.7018116E-422.1999995
CGAATTA603.725752E-521.58333415
CGTATAG450.003825515720.5555551
CGGTTAT450.003825515720.55555537
GCGGGTA656.9011476E-519.92307922
TTCGCCG1254.129106E-1019.24000224
ATTTTCG801.6163782E-518.515
GCCGGCA1501.2732926E-1118.49999815
TATTCCG1351.1514203E-917.8148165
CTAGACA1156.402843E-817.6956524
CGAACTA1054.7959475E-717.61904724
TTAACGG852.7226262E-517.41176435
CGCTCTC1502.5102054E-1017.26666629
TAAGACT2300.016.8913044
ATAATAC2450.016.6122443
TATCAAC45450.016.3630352
ATCAACG45450.016.2816283
CGAGTCG803.3820962E-416.187521
Produced by FastQC (version 0.11.5)