##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1019248
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870077743591352	33.0	33.0	33.0	33.0	33.0
2	31.986340910161218	33.0	33.0	33.0	33.0	33.0
3	31.69922629232532	33.0	33.0	33.0	27.0	33.0
4	31.976580773275984	33.0	33.0	33.0	33.0	33.0
5	32.076543687110494	33.0	33.0	33.0	33.0	33.0
6	35.23809122019371	37.0	37.0	37.0	33.0	37.0
7	35.38626713027644	37.0	37.0	37.0	33.0	37.0
8	35.530569596408334	37.0	37.0	37.0	33.0	37.0
9	35.640534982653875	37.0	37.0	37.0	33.0	37.0
10	35.65100250380673	37.0	37.0	37.0	33.0	37.0
11	35.6749907775144	37.0	37.0	37.0	33.0	37.0
12	35.66683770780026	37.0	37.0	37.0	33.0	37.0
13	35.66140429022181	37.0	37.0	37.0	33.0	37.0
14	35.64285826413199	37.0	37.0	37.0	33.0	37.0
15	35.669450418347644	37.0	37.0	37.0	33.0	37.0
16	35.64111089744596	37.0	37.0	37.0	33.0	37.0
17	35.64997821923614	37.0	37.0	37.0	33.0	37.0
18	35.624733136586975	37.0	37.0	37.0	33.0	37.0
19	35.61566861058349	37.0	37.0	37.0	33.0	37.0
20	35.62803655243866	37.0	37.0	37.0	33.0	37.0
21	35.60908826899832	37.0	37.0	37.0	33.0	37.0
22	35.52503610505	37.0	37.0	37.0	33.0	37.0
23	35.5743626673783	37.0	37.0	37.0	33.0	37.0
24	35.56942569423732	37.0	37.0	37.0	33.0	37.0
25	35.59005364739494	37.0	37.0	37.0	33.0	37.0
26	35.47739313690093	37.0	37.0	37.0	33.0	37.0
27	35.491683083999185	37.0	37.0	37.0	33.0	37.0
28	35.50839736747092	37.0	37.0	37.0	33.0	37.0
29	35.51275842581982	37.0	37.0	37.0	33.0	37.0
30	35.51481680611588	37.0	37.0	37.0	33.0	37.0
31	35.51089823085255	37.0	37.0	37.0	33.0	37.0
32	35.500813344740436	37.0	37.0	37.0	33.0	37.0
33	35.49765219063466	37.0	37.0	37.0	33.0	37.0
34	35.48388910255404	37.0	37.0	37.0	33.0	37.0
35	35.41064981241072	37.0	37.0	37.0	33.0	37.0
36	35.44562265513398	37.0	37.0	37.0	33.0	37.0
37	35.43800821782334	37.0	37.0	37.0	33.0	37.0
38	35.43260815817151	37.0	37.0	37.0	33.0	37.0
39	35.33049365806948	37.0	37.0	37.0	33.0	37.0
40	35.11889648054252	37.0	37.0	37.0	33.0	37.0
41	35.28620512377753	37.0	37.0	37.0	33.0	37.0
42	35.32858440732775	37.0	37.0	37.0	33.0	37.0
43	34.99592248402744	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	32.0
16	50.0
17	36.0
18	34.0
19	31.0
20	49.0
21	149.0
22	422.0
23	1073.0
24	2135.0
25	3796.0
26	6363.0
27	9675.0
28	13904.0
29	18586.0
30	24603.0
31	31374.0
32	40611.0
33	53546.0
34	77286.0
35	156102.0
36	579385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44388804294931	20.626677707486305	14.289456540508297	23.63997770905609
2	15.563140668414361	22.33313187761958	39.86507699794358	22.23865045602248
3	17.790469051693016	27.671773699825753	30.996283534527418	23.541473713953817
4	12.342629075553742	18.233148360359795	40.140868561920165	29.283354002166302
5	13.451093355101015	37.414741064000125	35.22665730028413	13.907508280614728
6	28.25946187777656	40.49887760388051	17.793019951964588	13.44864056637835
7	25.452588575106354	33.22439681019732	23.57414485973973	17.748869754956594
8	24.194798518123164	35.74184104359293	21.163740326201278	18.899620112082633
9	26.091392870037517	15.83628322057046	20.498936470809852	37.573387438582174
10	14.79728191764909	28.512197227760073	33.88674787686608	22.803772977724755
11	32.71088096321994	24.558596141469007	24.559773480055885	18.17074941525517
12	22.770709385743217	26.206477716905013	30.78593237367157	20.236880523680203
13	28.141826130637487	22.90512220774532	26.634145958589077	22.318905703028115
14	22.07980785834262	21.84169112914619	28.581463981288163	27.49703703122302
15	23.512040249281824	28.833512550429337	25.446898105269767	22.207549095019072
16	22.14338414203413	29.1977026199708	25.588767404988776	23.070145833006293
17	21.933425427373905	27.901845282011834	26.908367737783152	23.256361552831105
18	22.971347503257302	26.959189520116794	28.35884887681899	21.710614099806914
19	24.697620206269722	26.419674112679154	28.51523868577618	20.367466995274945
20	24.557124468235404	26.154576707533394	28.250141280630427	21.03815754360077
21	22.817606706120593	27.23939610379417	27.836993548184548	22.106003641900696
22	22.144070922876473	27.293553678790637	28.160271101831942	22.402104296500948
23	22.20990377219283	27.020214903536726	28.578913081016594	22.19096824325385
24	22.6316853209425	27.119013233285717	28.05028805550759	22.199013390264195
25	22.74333626359826	26.985189080577054	28.361792223286187	21.9096824325385
26	22.629134420670926	27.048274806524024	28.064808564745775	22.257782208059275
27	22.622462835345274	27.029535500682854	27.9470747060578	22.40092695791407
28	22.08216253551638	27.32131924713122	28.2733937177213	22.3231244996311
29	22.850179740357596	27.262648540885042	27.7595835360972	22.12758818266016
30	22.49020846741912	27.333681302293456	28.19833838280772	21.97777184747971
31	22.80916911291462	26.915333657755518	28.099834387705446	22.175662841624412
32	21.77340549110717	26.993528562234122	28.413889455755616	22.819176490903097
33	22.13828234149098	26.840474545939752	28.699099728427235	22.322143384142034
34	22.225994066213524	26.862647755992654	28.170670926016044	22.74068725177778
35	22.381108425034927	27.48693154168563	28.045971147355697	22.08598888592374
36	22.64041724879519	27.122741472144167	28.07098959232689	22.16585168673375
37	22.989498139805033	26.741774327739666	27.729757625229578	22.53896990722572
38	22.85851922201466	27.036501420655227	27.855340407830088	22.24963894950002
39	22.460480668100402	26.850874370123854	28.128286736888374	22.560358224887366
40	22.446254493508942	27.130982842252326	28.226986955088456	22.195775709150276
41	22.368255812128158	26.68987331836805	28.40260662763135	22.539264241872438
42	22.315079352620756	26.990388992669107	28.142414799930933	22.55211685477921
43	22.30291352055633	26.745895012793746	28.241899910522267	22.709291556127656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	69.5
2	92.0
3	306.0
4	520.0
5	520.0
6	663.5
7	807.0
8	787.0
9	767.0
10	1017.5
11	1268.0
12	1268.0
13	2014.0
14	2760.0
15	5305.0
16	7850.0
17	9202.5
18	10555.0
19	10555.0
20	10256.0
21	9957.0
22	11343.5
23	12730.0
24	15998.5
25	19267.0
26	19267.0
27	23642.5
28	28018.0
29	33640.0
30	39262.0
31	43158.0
32	47054.0
33	47054.0
34	51543.5
35	56033.0
36	58823.0
37	61613.0
38	64924.0
39	68235.0
40	68235.0
41	70735.5
42	73236.0
43	71628.0
44	70020.0
45	70596.5
46	71173.0
47	71173.0
48	70579.5
49	69986.0
50	70907.5
51	71829.0
52	71773.0
53	71717.0
54	71717.0
55	65376.0
56	59035.0
57	53427.0
58	47819.0
59	42332.0
60	36845.0
61	36845.0
62	32204.5
63	27564.0
64	22882.5
65	18201.0
66	15373.5
67	12546.0
68	12546.0
69	10443.5
70	8341.0
71	7094.5
72	5848.0
73	4673.5
74	3499.0
75	3499.0
76	2698.5
77	1898.0
78	1478.5
79	1059.0
80	841.5
81	624.0
82	624.0
83	516.0
84	408.0
85	387.0
86	366.0
87	291.0
88	216.0
89	216.0
90	175.0
91	134.0
92	87.5
93	41.0
94	24.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1019248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.54751453286636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4558014925537	54.70143014838978
2	7.834790865924416	9.80093391096752
3	1.778894229502681	3.3379623801675313
4	0.7466102231257242	1.8679445512537134
5	0.44237760824417843	1.3834809940333705
6	0.2743298756908115	1.0295191131942274
7	0.20535953510860064	0.8991309954667512
8	0.15038202418120333	0.7524817474364541
9	0.1128677718600628	0.6353638740638571
>10	0.8027244247627188	10.151620980333403
>50	0.12097827947132028	5.234327321722756
>100	0.07203397414770323	8.022941385778678
>500	0.002374746189091734	0.971415445402511
>1k	3.166328252122312E-4	0.6249340342441637
>5k	1.583164126061156E-4	0.5865131175452674
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5923	0.5811147041740578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3977	0.3901896300017268	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2334	0.22899235514810917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.962230978132898E-4	0.0	0.0
2	0.0	0.0	1.962230978132898E-4	0.0	0.0
3	0.0	0.0	2.943346467199347E-4	0.0	0.0
4	0.0	0.0	3.924461956265796E-4	0.0	0.0
5	0.0	0.0	5.886692934398694E-4	0.0	0.0
6	0.0	0.0	0.0012754501357863837	0.0	0.0
7	0.0	0.0	0.003041458016105992	0.0	0.0
8	0.0	0.0	0.0034339042117325714	0.0	0.0
9	0.0	0.0	0.00421879660298573	4.905577445332245E-4	0.0
10	0.0	0.0	0.004513131249705665	4.905577445332245E-4	0.0
11	0.0	0.0	0.006279139130025274	4.905577445332245E-4	0.0
12	0.0	0.0	0.007064031521278433	4.905577445332245E-4	0.0
13	0.0	0.0	0.007260254619091722	4.905577445332245E-4	0.0
14	0.0	0.0	0.007456477716905012	5.886692934398694E-4	0.0
15	0.0	0.0	0.007750812363624947	5.886692934398694E-4	0.0
16	0.0	0.0	0.008633816303784752	5.886692934398694E-4	0.0
17	0.0	0.0	0.008830039401598041	6.867808423465143E-4	0.0
18	0.0	0.0	0.009124374048317976	6.867808423465143E-4	0.0
19	0.0	0.0	0.00941870869503791	6.867808423465143E-4	0.0
20	0.0	0.0	0.009811154890664489	6.867808423465143E-4	0.0
21	0.0	0.0	0.010399824184104359	9.81115489066449E-4	0.0
22	0.0	0.0	0.010497935733011004	0.0015697847825063185	0.0
23	0.0	0.0	0.010497935733011004	0.001864119429226253	0.0
24	0.0	0.0	0.010497935733011004	0.0024527887226661223	0.0
25	0.0	0.0	0.01059604728191765	0.0024527887226661223	0.0
26	0.0	0.0	0.01059604728191765	0.003139569565012637	0.0
27	0.0	0.0	0.010694158830824294	0.003728238858452506	0.0
28	0.0	0.0	0.010694158830824294	0.011969608966610678	0.0
29	0.0	0.0	0.010694158830824294	0.03041458016105992	0.0
30	0.0	0.0	0.010694158830824294	0.05435379809428127	0.0
31	0.0	0.0	0.010694158830824294	0.1173414124923473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1120	0.0	24.776785	1
GTATCAA	3130	0.0	23.9377	1
TAGACCG	50	2.7018116E-4	22.199999	5
CGAATTA	60	3.725752E-5	21.583334	15
CGTATAG	45	0.0038255157	20.555555	1
CGGTTAT	45	0.0038255157	20.555555	37
GCGGGTA	65	6.9011476E-5	19.923079	22
TTCGCCG	125	4.129106E-10	19.240002	24
ATTTTCG	80	1.6163782E-5	18.5	15
GCCGGCA	150	1.2732926E-11	18.499998	15
TATTCCG	135	1.1514203E-9	17.814816	5
CTAGACA	115	6.402843E-8	17.695652	4
CGAACTA	105	4.7959475E-7	17.619047	24
TTAACGG	85	2.7226262E-5	17.411764	35
CGCTCTC	150	2.5102054E-10	17.266666	29
TAAGACT	230	0.0	16.891304	4
ATAATAC	245	0.0	16.612244	3
TATCAAC	4545	0.0	16.363035	2
ATCAACG	4545	0.0	16.281628	3
CGAGTCG	80	3.3820962E-4	16.1875	21
>>END_MODULE