FastQCFastQC Report
Fri 10 Feb 2017
ERR1633419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560585
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141712.5278949668649715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT106481.899444330476199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54230.9673822881454195No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA32550.580643434983098No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27860.49698083252316777No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT27290.4868128829704684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.46094704638904No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24150.4307999678906856No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC23330.4161723913411883No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA22060.3935174861974544No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG19430.3466022101911396No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17620.3143145107343222No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA17020.303611405942007No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15980.2850593576353274No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15560.27756718428070676No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.27364271252352457No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC14610.26062060169287443No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14410.257052900095436No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13830.24670656546286468No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13520.24117662798683517No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC13410.23921439210824405No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC13090.23350606955234265No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13030.23243575907311112No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12900.23011675303477616No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12820.22868967239580082No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12720.22690582159708164No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG12510.22315973491977129No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12090.2156675615651507No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11660.20799700313065814No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11560.20621315233193896No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11310.20175352533514096No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10850.19354781166103266No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10820.1930126564214169No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10620.18944495482397852No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10570.18855302942461893No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10450.1864124084661559No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10400.18552048306679628No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT10270.18320147702846132No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10190.181774396389486No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9880.1762444589134565No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9860.17588768875371263No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT9660.17231998715627425No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9660.17231998715627425No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9580.17089290651729888No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9450.16857390047896392No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9330.1664332795205009No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9260.16518458396139749No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9150.16322234808280633No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9010.16072495696459946No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8980.16018980172498373No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8970.1600114166451118No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8950.15965464648536795No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA8900.15876272108600836No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8860.1580491807665207No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8860.1580491807665207No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA8810.1571572553671611No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG8770.1564437150476734No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG8710.1553734045684419No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT8710.1553734045684419No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8690.15501663440869806No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8670.15465986424895423No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC8660.1544814791690823No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8610.1535895537697227No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8320.14841638645343702No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8300.14805961629369319No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8240.14698930581446168No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8210.14645415057484593No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8210.14645415057484593No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.14591899533523017No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8030.14324321913715138No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7880.14056744293907256No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG7770.13860520706048146No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.13592943086240267No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7460.13307526958445196No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7430.1325401143448362No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7300.13022110830650124No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT7130.1271885619486786No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7080.126296636549319No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7010.12504794099021557No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6930.12362086035124023No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6890.12290732003175253No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.12272893495188061No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA6870.12255054987200871No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6780.12094508415316144No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6740.12023154383367377No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT6670.11898284827457031No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6630.11826930795508263No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6550.11684222731610729No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6430.11470160635764426No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG6380.11380968095828464No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6300.1123826003193093No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6290.11220421523943738No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6290.11220421523943738No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6270.11184744507969353No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG6230.11113390476020586No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC6230.11113390476020586No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6210.11077713460046201No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6180.11024197936084627No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA6140.10952843904135857No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6100.1088148987218709No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC6050.10792297332251131No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6050.10792297332251131No Hit
GTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCG5980.10667427776340788No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5890.10506881204456059No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA5870.10471204188481677No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5770.10292819108609756No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5770.10292819108609756No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5620.10025241488801875No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5610.10007402980814685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC451.0782969E-732.888891
GAACCGC401.5981313E-632.37526
AACCGCT352.3861412E-531.71428727
ATTTCCT352.3861412E-531.71428718
GATACAC250.00549397229.5999983
CCGACAT250.00549397229.59999837
GCACCGT454.003441E-628.7777796
TATTTCC405.934783E-527.75000217
TGGAACC405.934783E-527.75000224
CTATTTC405.934783E-527.75000216
ACCTTTC405.934783E-527.75000222
TGTCAAC556.2521394E-726.909092
TATGAGG358.863703E-426.4285724
ATGAGGT358.863703E-426.4285725
CCGTAGT509.080246E-625.8999989
CGTAGTG509.080246E-625.89999810
ACCGTAG509.080246E-625.8999988
ACAACGC659.37398E-825.6153833
TATACGC451.3220306E-424.6666684
CGCTTCG451.3220306E-424.66666830
Produced by FastQC (version 0.11.5)