FastQCFastQC Report
Fri 10 Feb 2017
ERR1633418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760391
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42350.5569503058295009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31350.4122878887309292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16460.2164675804947718No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.10178973712208587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG200.001841544437.035
GGTATCA5750.027.9913041
GTATCAA18850.024.4376661
CTCGTAT400.001930589423.12536
TCGAACA400.001930589423.12513
CCGGTTC502.700937E-422.19999921
CGGTTCG450.00382463420.55555722
GCTTCGC1101.7516868E-920.18181822
AATACCG555.1408925E-420.1818185
AGCTTCG1052.2564564E-819.3809521
ATCTCGT801.6154981E-518.537
GGTTCGC500.007032746418.49999823
CCCGGTT500.007032746418.49999820
TTACTCG650.001579268817.0769234
TAACGGC650.001579268817.07692336
GTCGTGC650.001579268817.0769238
CGAGTCG1201.0398071E-716.95833221
CCGTCCG1201.0398071E-716.95833228
TTCGCCG1201.0398071E-716.95833224
TGCTCGC1452.9740477E-916.58620610
Produced by FastQC (version 0.11.5)