##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	760391
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68997923436758	33.0	33.0	33.0	27.0	33.0
2	31.836130359249385	33.0	33.0	33.0	33.0	33.0
3	31.554234597726698	33.0	33.0	33.0	27.0	33.0
4	31.846911654661877	33.0	33.0	33.0	27.0	33.0
5	31.94593307916585	33.0	33.0	33.0	33.0	33.0
6	35.072103694020576	37.0	37.0	37.0	33.0	37.0
7	35.222738038719555	37.0	37.0	37.0	33.0	37.0
8	35.359828035839456	37.0	37.0	37.0	33.0	37.0
9	35.48604073430643	37.0	37.0	37.0	33.0	37.0
10	35.49693381431395	37.0	37.0	37.0	33.0	37.0
11	35.5028491920604	37.0	37.0	37.0	33.0	37.0
12	35.514353799558386	37.0	37.0	37.0	33.0	37.0
13	35.50487446589978	37.0	37.0	37.0	33.0	37.0
14	35.48828826222299	37.0	37.0	37.0	33.0	37.0
15	35.49667342196317	37.0	37.0	37.0	33.0	37.0
16	35.477046677301544	37.0	37.0	37.0	33.0	37.0
17	35.4737102359181	37.0	37.0	37.0	33.0	37.0
18	35.458599588895716	37.0	37.0	37.0	33.0	37.0
19	35.45796570448624	37.0	37.0	37.0	33.0	37.0
20	35.45499223425843	37.0	37.0	37.0	33.0	37.0
21	35.45235674804147	37.0	37.0	37.0	33.0	37.0
22	35.36143115844349	37.0	37.0	37.0	33.0	37.0
23	35.39711017095152	37.0	37.0	37.0	33.0	37.0
24	35.37822383484286	37.0	37.0	37.0	33.0	37.0
25	35.40855033791826	37.0	37.0	37.0	33.0	37.0
26	35.30933559182052	37.0	37.0	37.0	33.0	37.0
27	35.31063623846153	37.0	37.0	37.0	33.0	37.0
28	35.31987096112395	37.0	37.0	37.0	33.0	37.0
29	35.3215937590003	37.0	37.0	37.0	33.0	37.0
30	35.32994341069266	37.0	37.0	37.0	33.0	37.0
31	35.33087977106515	37.0	37.0	37.0	33.0	37.0
32	35.30863332154115	37.0	37.0	37.0	33.0	37.0
33	35.300822866130716	37.0	37.0	37.0	33.0	37.0
34	35.271727308713544	37.0	37.0	37.0	33.0	37.0
35	35.20413445188068	37.0	37.0	37.0	33.0	37.0
36	35.23698860191664	37.0	37.0	37.0	33.0	37.0
37	35.22662682751374	37.0	37.0	37.0	33.0	37.0
38	35.21579818803747	37.0	37.0	37.0	33.0	37.0
39	35.11334431890961	37.0	37.0	37.0	33.0	37.0
40	34.899397809811006	37.0	37.0	37.0	27.0	37.0
41	35.063260874997205	37.0	37.0	37.0	27.0	37.0
42	35.108524430194464	37.0	37.0	37.0	33.0	37.0
43	34.753138845672815	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	22.0
16	38.0
17	44.0
18	25.0
19	39.0
20	68.0
21	175.0
22	447.0
23	1022.0
24	2082.0
25	3701.0
26	5726.0
27	8654.0
28	11938.0
29	15886.0
30	20395.0
31	26156.0
32	33245.0
33	43102.0
34	62140.0
35	122757.0
36	402725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96734443200932	20.89503952571769	14.245039722984624	23.892576319288366
2	15.590926247154425	22.439639606465622	39.54255113487666	22.426883011503293
3	17.699446732010244	27.771238744277614	30.83413664811919	23.69517787559295
4	12.336153373724834	18.39527295825437	40.19576770372085	29.072805964299945
5	13.31696456165315	37.465461847917716	35.635350760332514	13.58222283009662
6	28.156435307624626	40.748115114460845	17.967729760083955	13.127719817830563
7	25.306191156917958	33.0067031303632	24.003834869165992	17.683270843552855
8	23.976217498628998	36.11812869957693	21.27537017139866	18.630283630395414
9	25.781209930154354	15.720333354813508	20.711055233425963	37.787401481606175
10	14.442832700544853	28.69615763469057	34.44385848859337	22.417151176171206
11	32.72408537186789	24.50305171944434	24.74516400115204	18.02769890753573
12	22.208837295549262	26.29870684950243	31.34308533372962	20.14937052121869
13	27.894464821387942	22.70095253626095	27.04437585400143	22.36020678834968
14	21.875456179781192	21.64781013978335	28.79689528150649	27.679838398928975
15	23.15387741306775	29.232066134396646	25.318158684150653	22.295897768384947
16	21.987372286100175	29.20760503477816	25.933236979396124	22.871785699725535
17	21.59178633097972	28.043730133576016	27.420761161034257	22.943722374410008
18	22.544059569353138	27.37368011983309	28.545182675754972	21.537077635058804
19	24.058938098951725	26.658258711636513	28.79202936384044	20.490773825571317
20	23.975165408322823	26.552655147154557	28.426427982445873	21.045751462076748
21	22.498688174899492	27.356320629781255	28.191811844169646	21.953179351149608
22	22.040634357850106	27.344090079972016	28.424981358274888	22.19029420390299
23	22.023011845221735	27.210869144953058	28.790582739669457	21.97553627015575
24	22.080876812061163	27.417999423980554	28.368563015606448	22.132560748351835
25	22.38072254932002	27.277545368106672	28.785453799426875	21.556278283146433
26	22.444374012843394	27.347377862178803	28.264669097872012	21.94357902710579
27	22.36375759313301	27.23743442518389	28.171033060622758	22.22777492106035
28	21.94897098992492	27.331333485009683	28.682874994575158	22.036820530490235
29	22.408865965010108	27.340933809053503	28.146177427139456	22.104022798796937
30	22.26906946557758	27.457452810461984	28.419983929320576	21.853493794639864
31	22.549846066037077	27.12012635604577	28.42156206477983	21.908465513137322
32	21.689367706877118	27.269786202098658	28.57161644469753	22.469229646326696
33	21.735396657772117	27.110526032001957	28.997055462255606	22.15702184797032
34	22.04352760619208	26.931407657376273	28.536371419440787	22.48869331699086
35	22.021039175897663	27.51189848380636	28.564251812554332	21.902810527741646
36	22.478172413929148	27.220864002861685	28.366458834994102	21.934504748215065
37	22.694113949270836	26.90668353518124	28.218377124400472	22.180825391147447
38	22.518283356851935	27.260843434496202	28.1795812943604	22.041291914291463
39	22.220278777628877	27.047269102343403	28.284395791112733	22.44805632891499
40	21.859411802612076	27.330675928568326	28.718909087561528	22.091003181258063
41	22.101261061743234	26.839218244298003	28.603310665170945	22.456210028787822
42	21.964752344517493	27.28030710516037	28.36751092530027	22.387429625021863
43	22.139136312765405	26.829223386389373	28.54426209673707	22.48737820410815
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	62.0
2	77.0
3	243.0
4	409.0
5	409.0
6	532.5
7	656.0
8	603.5
9	551.0
10	778.0
11	1005.0
12	1005.0
13	1609.5
14	2214.0
15	4155.0
16	6096.0
17	7033.0
18	7970.0
19	7970.0
20	7788.5
21	7607.0
22	8835.5
23	10064.0
24	12547.5
25	15031.0
26	15031.0
27	18274.0
28	21517.0
29	25670.5
30	29824.0
31	32890.5
32	35957.0
33	35957.0
34	39461.5
35	42966.0
36	45475.5
37	47985.0
38	50152.5
39	52320.0
40	52320.0
41	53925.5
42	55531.0
43	54782.0
44	54033.0
45	54350.5
46	54668.0
47	54668.0
48	53372.5
49	52077.0
50	52318.0
51	52559.0
52	51790.5
53	51022.0
54	51022.0
55	46513.0
56	42004.0
57	37854.0
58	33704.0
59	29925.0
60	26146.0
61	26146.0
62	23175.5
63	20205.0
64	16495.0
65	12785.0
66	10792.5
67	8800.0
68	8800.0
69	7169.5
70	5539.0
71	4749.5
72	3960.0
73	3120.5
74	2281.0
75	2281.0
76	1737.0
77	1193.0
78	907.0
79	621.0
80	501.0
81	381.0
82	381.0
83	312.0
84	243.0
85	206.0
86	169.0
87	138.0
88	107.0
89	107.0
90	76.5
91	46.0
92	33.0
93	20.0
94	10.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	760391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.64169369902788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.1636766969967	58.75376737821376
2	7.317585517351268	9.75312585327531
3	1.6768582411503141	3.3524601985028957
4	0.7147994725755269	1.9054179003041973
5	0.4040875293335954	1.3464538678723204
6	0.2619449807102358	1.047387430229358
7	0.19130652203740833	0.8924293450970306
8	0.15788231111585527	0.841723569430196
9	0.12407727027648466	0.7441847496699288
>10	0.8636650877360045	11.619923989145809
>50	0.08055688826486226	3.639792244929702
>100	0.04236607197444645	4.665149779903411
>500	5.967052386767241E-4	0.24309943721137406
>1k	5.967052386767241E-4	1.1950842562146937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4235	0.5569503058295009	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3135	0.4122878887309292	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1646	0.2164675804947718	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	774	0.10178973712208587	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.630225765428576E-4	0.0	0.0
2	0.0	0.0	2.630225765428576E-4	0.0	0.0
3	0.0	0.0	5.260451530857152E-4	0.0	0.0
4	0.0	0.0	9.205790179000014E-4	0.0	0.0
5	0.0	0.0	0.001183601594442859	0.0	0.0
6	0.0	0.0	0.0021041806123428606	0.0	0.0
7	0.0	0.0	0.004734406377771436	0.0	0.0
8	1.315112882714288E-4	0.0	0.005786496683942866	0.0	0.0
9	1.315112882714288E-4	0.0	0.007627654719742869	3.9453386481428634E-4	0.0
10	3.9453386481428634E-4	0.0	0.008416722449371443	3.9453386481428634E-4	0.0
11	3.9453386481428634E-4	0.0	0.011572993367885732	5.260451530857152E-4	0.0
12	3.9453386481428634E-4	0.0	0.012099038520971448	5.260451530857152E-4	0.0
13	5.260451530857152E-4	0.0	0.013282640115414307	5.260451530857152E-4	0.0
14	5.260451530857152E-4	0.0	0.015255309439485738	5.260451530857152E-4	0.0
15	5.260451530857152E-4	0.0	0.01604437716911431	6.575564413571439E-4	0.0
16	5.260451530857152E-4	0.0	0.0173594900518286	6.575564413571439E-4	0.0
17	5.260451530857152E-4	0.0	0.017754023916642884	6.575564413571439E-4	0.0
18	5.260451530857152E-4	0.0	0.018148557781457172	6.575564413571439E-4	0.0
19	5.260451530857152E-4	0.0	0.018148557781457172	9.205790179000014E-4	0.0
20	5.260451530857152E-4	0.0	0.018674602934542886	0.001183601594442859	0.0
21	5.260451530857152E-4	0.0	0.01933215937590003	0.0013151128827142877	0.0
22	5.260451530857152E-4	0.0	0.019726693240714317	0.0021041806123428606	0.0
23	5.260451530857152E-4	0.0	0.020384249682071462	0.002235691900614289	0.0
24	5.260451530857152E-4	0.0	0.020384249682071462	0.0024987144771571467	0.0
25	5.260451530857152E-4	0.0	0.020384249682071462	0.0027617370537000043	0.0
26	5.260451530857152E-4	0.0	0.020384249682071462	0.0031562709185142908	0.0
27	5.260451530857152E-4	0.0	0.020384249682071462	0.004997428954314293	0.0
28	5.260451530857152E-4	0.0	0.020384249682071462	0.013808685268500022	0.0
29	5.260451530857152E-4	0.0	0.02051576097034289	0.02906399470798576	0.0
30	5.260451530857152E-4	0.0	0.02051576097034289	0.04787010893080008	0.0
31	5.260451530857152E-4	0.0	0.02051576097034289	0.10639263221158587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTACG	20	0.0018415444	37.0	35
GGTATCA	575	0.0	27.991304	1
GTATCAA	1885	0.0	24.437666	1
CTCGTAT	40	0.0019305894	23.125	36
TCGAACA	40	0.0019305894	23.125	13
CCGGTTC	50	2.700937E-4	22.199999	21
CGGTTCG	45	0.003824634	20.555557	22
GCTTCGC	110	1.7516868E-9	20.181818	22
AATACCG	55	5.1408925E-4	20.181818	5
AGCTTCG	105	2.2564564E-8	19.38095	21
ATCTCGT	80	1.6154981E-5	18.5	37
GGTTCGC	50	0.0070327464	18.499998	23
CCCGGTT	50	0.0070327464	18.499998	20
TTACTCG	65	0.0015792688	17.076923	4
TAACGGC	65	0.0015792688	17.076923	36
GTCGTGC	65	0.0015792688	17.076923	8
CGAGTCG	120	1.0398071E-7	16.958332	21
CCGTCCG	120	1.0398071E-7	16.958332	28
TTCGCCG	120	1.0398071E-7	16.958332	24
TGCTCGC	145	2.9740477E-9	16.586206	10
>>END_MODULE