FastQCFastQC Report
Fri 10 Feb 2017
ERR1633417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706359
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50650.7170574736076132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34680.4909684735382433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19940.2822927151774098No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8560.12118483660574864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.11948598375613534No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7580.10731087166724004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTT251.2331443E-436.99999616
TATACGG352.3870141E-531.71428732
GACGTAT454.005382E-628.77777728
CACGAAA454.005382E-628.77777718
ACGTATA454.005382E-628.77777729
GGTATCA10350.024.1304361
TGCTCCG851.9736035E-923.94117728
ATACGGT400.001930453823.12500233
GGACCGT903.8216967E-922.611116
GTATCAA24850.022.3340051
CCGTTTA753.7365498E-722.227
GACCGTT806.952305E-720.81257
TCGCCAT909.465293E-820.55555513
ACGGTGT450.0038243720.55555535
TTACCGG656.897318E-519.92307731
ACACGAA759.257481E-619.73333417
GTCGCCA951.673834E-719.47368412
TTCGCCG1052.2557288E-819.38095324
CGTATAC701.218353E-418.530
TTGCGGT701.218353E-418.535
Produced by FastQC (version 0.11.5)