##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706359
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80686166665959	33.0	33.0	33.0	27.0	33.0
2	31.92770248556329	33.0	33.0	33.0	33.0	33.0
3	31.646202568382368	33.0	33.0	33.0	27.0	33.0
4	31.929578302251404	33.0	33.0	33.0	33.0	33.0
5	32.025763103464385	33.0	33.0	33.0	33.0	33.0
6	35.176421621300214	37.0	37.0	37.0	33.0	37.0
7	35.32839250296237	37.0	37.0	37.0	33.0	37.0
8	35.465968438145474	37.0	37.0	37.0	33.0	37.0
9	35.58521375108125	37.0	37.0	37.0	33.0	37.0
10	35.60348491347884	37.0	37.0	37.0	33.0	37.0
11	35.61597856047704	37.0	37.0	37.0	33.0	37.0
12	35.61523672806604	37.0	37.0	37.0	33.0	37.0
13	35.59675037764083	37.0	37.0	37.0	33.0	37.0
14	35.58620899570898	37.0	37.0	37.0	33.0	37.0
15	35.613096173475526	37.0	37.0	37.0	33.0	37.0
16	35.59328613353833	37.0	37.0	37.0	33.0	37.0
17	35.58697914233414	37.0	37.0	37.0	33.0	37.0
18	35.56993115398827	37.0	37.0	37.0	33.0	37.0
19	35.56116648899497	37.0	37.0	37.0	33.0	37.0
20	35.56062144037239	37.0	37.0	37.0	33.0	37.0
21	35.55447725589962	37.0	37.0	37.0	33.0	37.0
22	35.45228559415255	37.0	37.0	37.0	33.0	37.0
23	35.498082419846	37.0	37.0	37.0	33.0	37.0
24	35.498198508124055	37.0	37.0	37.0	33.0	37.0
25	35.51550981866161	37.0	37.0	37.0	33.0	37.0
26	35.41010874073948	37.0	37.0	37.0	33.0	37.0
27	35.42429699345517	37.0	37.0	37.0	33.0	37.0
28	35.435617016276424	37.0	37.0	37.0	33.0	37.0
29	35.44407305633538	37.0	37.0	37.0	33.0	37.0
30	35.44988030165964	37.0	37.0	37.0	33.0	37.0
31	35.438039297297834	37.0	37.0	37.0	33.0	37.0
32	35.42887257046346	37.0	37.0	37.0	33.0	37.0
33	35.41630530650845	37.0	37.0	37.0	33.0	37.0
34	35.390826760896374	37.0	37.0	37.0	33.0	37.0
35	35.33052739471005	37.0	37.0	37.0	33.0	37.0
36	35.34874051296862	37.0	37.0	37.0	33.0	37.0
37	35.34230327637929	37.0	37.0	37.0	33.0	37.0
38	35.31312123155506	37.0	37.0	37.0	33.0	37.0
39	35.21574864905806	37.0	37.0	37.0	33.0	37.0
40	34.99953281546636	37.0	37.0	37.0	27.0	37.0
41	35.160605301270316	37.0	37.0	37.0	33.0	37.0
42	35.19945948165168	37.0	37.0	37.0	33.0	37.0
43	34.8515740579507	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	32.0
16	36.0
17	32.0
18	31.0
19	30.0
20	44.0
21	119.0
22	351.0
23	783.0
24	1685.0
25	2908.0
26	5018.0
27	7277.0
28	10213.0
29	13639.0
30	18042.0
31	22858.0
32	29136.0
33	37873.0
34	55004.0
35	109772.0
36	391469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84246254383394	20.699247832900834	14.236670021901043	23.22161960136418
2	15.602406141919337	22.5985653187685	39.916671267726464	21.882357271585697
3	17.86655227724146	28.04423812820393	30.924784705794078	23.164424888760532
4	12.146373161522682	18.157339256666933	40.45917161103632	29.23711597077407
5	13.405506265227737	37.48915211669986	35.135108351419035	13.97023326665336
6	28.053440247805998	40.72957235626643	17.647400259641344	13.569587136286223
7	25.231928806739916	33.28434974283615	23.583758400473414	17.89996304995052
8	24.096245676773425	35.92861420325925	20.999520074069984	18.97562004589734
9	25.960170394940818	15.648558311000496	20.694717558635197	37.696553735423485
10	14.680353757791718	28.499389120829495	33.90697931221943	22.913277809159364
11	32.83316274019302	24.74081876213087	24.335076073214896	18.090942424461215
12	22.830741874882317	26.234535130153365	30.963008894910377	19.971714100053937
13	28.27584840003454	23.084437233757903	26.606159191006274	22.03355517520128
14	22.052100985476226	21.78198338238771	28.85133480284105	27.31458082929502
15	23.42703922509659	28.974784776579614	25.719641145649735	21.878534852674065
16	21.816243581521576	29.341736992096084	25.767492167580507	23.074527258801826
17	21.679061213915304	27.788136061124725	27.047577789764127	23.48522493519584
18	22.77057416979185	26.75537509963064	28.825427296884442	21.648623433693064
19	24.544742829071335	26.205937773851538	28.96728150982716	20.282037887249967
20	24.424407418890397	25.94035044502866	28.726893831606876	20.90834830447407
21	22.633816515397978	27.227373049681535	28.29326164174308	21.845548793177407
22	21.965317919075144	27.298441727223693	28.59579902004505	22.140441333656117
23	22.073761359308794	26.9541408830354	29.107436869920257	21.864660887735557
24	22.346993525954932	27.075750432853553	28.589003608646596	21.988252432544925
25	22.298576219740955	27.128698013333164	28.88757699696613	21.68514876995975
26	22.452322402630955	27.0822627021104	28.52175735001607	21.943657545242576
27	22.374458313690347	27.089199684579658	28.491461140864633	22.04488086086537
28	21.883489840152105	27.192404995193662	28.77247971640483	22.151625448249405
29	22.627021103999525	27.00949517171863	28.412322912286815	21.95116081199503
30	22.39243783968209	27.204296965140955	28.852892084619857	21.550373110557096
31	22.595026041998473	26.987268513602857	28.409916204083192	22.007789240315475
32	21.339573786134245	26.956830733380617	28.9352864478261	22.768309032659033
33	21.70142944310188	26.934887217406445	29.421299933886306	21.942383405605366
34	21.879384279098872	26.791758864826527	28.783380688856514	22.545476167218087
35	21.96517634800434	27.489137959592785	28.624396376346873	21.921289316056
36	22.378988587955984	27.225391054690316	28.617742536019218	21.777877821334478
37	22.67642940770911	26.946496045212136	28.207044859625203	22.170029687453546
38	22.457277390108995	27.176549035263935	28.21822897421849	22.14794460040857
39	22.150492879682997	26.945788189858135	28.624821089559276	22.2788978408996
40	21.954558517694263	27.473423570733864	28.762711312519556	21.809306599052324
41	22.123594376230784	26.666610038238346	28.837885551114944	22.371910034415926
42	21.950028243428623	27.132803574386394	28.6538431590735	22.263325023111477
43	22.04275729480335	26.928374948149596	28.47857817342173	22.550289583625325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	64.0
2	79.0
3	305.5
4	532.0
5	532.0
6	617.5
7	703.0
8	696.0
9	689.0
10	908.0
11	1127.0
12	1127.0
13	1794.0
14	2461.0
15	4640.0
16	6819.0
17	7851.0
18	8883.0
19	8883.0
20	8432.5
21	7982.0
22	8651.0
23	9320.0
24	11692.5
25	14065.0
26	14065.0
27	17034.5
28	20004.0
29	23503.0
30	27002.0
31	29618.5
32	32235.0
33	32235.0
34	35203.0
35	38171.0
36	40147.5
37	42124.0
38	44154.0
39	46184.0
40	46184.0
41	47812.0
42	49440.0
43	49066.5
44	48693.0
45	48831.0
46	48969.0
47	48969.0
48	48837.0
49	48705.0
50	49279.0
51	49853.0
52	50053.0
53	50253.0
54	50253.0
55	46116.5
56	41980.0
57	37557.5
58	33135.0
59	29318.0
60	25501.0
61	25501.0
62	22188.5
63	18876.0
64	15212.5
65	11549.0
66	9703.0
67	7857.0
68	7857.0
69	6425.5
70	4994.0
71	4245.5
72	3497.0
73	2786.5
74	2076.0
75	2076.0
76	1551.0
77	1026.0
78	776.5
79	527.0
80	437.5
81	348.0
82	348.0
83	288.5
84	229.0
85	214.5
86	200.0
87	168.5
88	137.0
89	137.0
90	100.0
91	63.0
92	41.5
93	20.0
94	11.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	706359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.43190380011402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.29216148460205	56.0053989359541
2	7.331711851228928	9.301288816746185
3	1.617928794095301	3.078849108674629
4	0.6687461013770122	1.6967935347699172
5	0.38721080076140935	1.2280759132131418
6	0.2507060660707011	0.9541657839061045
7	0.17348577731882434	0.7703173196302967
8	0.13278859047094427	0.6738426477204538
9	0.11342848322057522	0.6475486172256337
>10	0.8551426076304415	11.393436170192418
>50	0.11102339209362623	4.832216704415729
>100	0.06340948579932809	7.249235911026841
>500	0.0015795957316166013	0.6620143368153704
>1k	4.513130661761718E-4	0.781821039499579
>5k	2.256565330880859E-4	0.7249951602096976
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5065	0.7170574736076132	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3468	0.4909684735382433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1994	0.2822927151774098	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	856	0.12118483660574864	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.11948598375613534	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	758	0.10731087166724004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.4157107080110823E-4	0.0	0.0
3	0.0	0.0	1.4157107080110823E-4	0.0	0.0
4	0.0	0.0	1.4157107080110823E-4	1.4157107080110823E-4	0.0
5	1.4157107080110823E-4	0.0	1.4157107080110823E-4	1.4157107080110823E-4	0.0
6	1.4157107080110823E-4	0.0	0.001981994991215515	1.4157107080110823E-4	0.0
7	1.4157107080110823E-4	0.0	0.0049549874780387875	1.4157107080110823E-4	0.0
8	1.4157107080110823E-4	0.0	0.005945984973646545	1.4157107080110823E-4	0.0
9	1.4157107080110823E-4	0.0	0.0069369824692543025	5.662842832044329E-4	0.0
10	1.4157107080110823E-4	0.0	0.00792797996486206	7.078553540055411E-4	0.0
11	1.4157107080110823E-4	0.0	0.010900972451685333	8.494264248066493E-4	0.0
12	1.4157107080110823E-4	0.0	0.011608827805690874	9.909974956077575E-4	0.0
13	1.4157107080110823E-4	0.0	0.012458254230497524	9.909974956077575E-4	0.0
14	1.4157107080110823E-4	0.0	0.012882967442900848	9.909974956077575E-4	0.0
15	1.4157107080110823E-4	0.0	0.014157107080110821	0.001274139637209974	0.0
16	1.4157107080110823E-4	0.0	0.014723391363315255	0.0014157107080110822	0.0
17	1.4157107080110823E-4	0.0	0.01514810457571858	0.0014157107080110822	0.0
18	2.8314214160221645E-4	0.0	0.015431246717320796	0.0014157107080110822	0.0
19	2.8314214160221645E-4	0.0	0.015572817788121904	0.0014157107080110822	0.0
20	2.8314214160221645E-4	0.0	0.016139102071326336	0.0014157107080110822	0.0
21	2.8314214160221645E-4	0.0	0.016422244212928552	0.0016988528496132986	0.0
22	2.8314214160221645E-4	0.0	0.016846957425331877	0.002123566062016623	0.0
23	2.8314214160221645E-4	0.0	0.016846957425331877	0.0028314214160221644	0.0
24	2.8314214160221645E-4	0.0	0.017130099566934093	0.0035392767700277053	0.0
25	2.8314214160221645E-4	0.0	0.017130099566934093	0.0035392767700277053	0.0
26	2.8314214160221645E-4	0.0	0.017130099566934093	0.004247132124033246	0.0
27	2.8314214160221645E-4	0.0	0.017271670637735204	0.005238129619641004	0.0
28	2.8314214160221645E-4	0.0	0.017271670637735204	0.012458254230497524	0.0
29	2.8314214160221645E-4	0.0	0.017271670637735204	0.030437780222238267	0.0
30	2.8314214160221645E-4	0.0	0.017271670637735204	0.05492957547082999	0.0
31	2.8314214160221645E-4	0.0	0.017271670637735204	0.1084434402336489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTT	25	1.2331443E-4	36.999996	16
TATACGG	35	2.3870141E-5	31.714287	32
GACGTAT	45	4.005382E-6	28.777777	28
CACGAAA	45	4.005382E-6	28.777777	18
ACGTATA	45	4.005382E-6	28.777777	29
GGTATCA	1035	0.0	24.130436	1
TGCTCCG	85	1.9736035E-9	23.941177	28
ATACGGT	40	0.0019304538	23.125002	33
GGACCGT	90	3.8216967E-9	22.61111	6
GTATCAA	2485	0.0	22.334005	1
CCGTTTA	75	3.7365498E-7	22.2	27
GACCGTT	80	6.952305E-7	20.8125	7
TCGCCAT	90	9.465293E-8	20.555555	13
ACGGTGT	45	0.00382437	20.555555	35
TTACCGG	65	6.897318E-5	19.923077	31
ACACGAA	75	9.257481E-6	19.733334	17
GTCGCCA	95	1.673834E-7	19.473684	12
TTCGCCG	105	2.2557288E-8	19.380953	24
CGTATAC	70	1.218353E-4	18.5	30
TTGCGGT	70	1.218353E-4	18.5	35
>>END_MODULE