FastQCFastQC Report
Fri 10 Feb 2017
ERR1633416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452822
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129402.857635008899744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99642.200423124318165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54261.1982633352619794No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23470.5183052060191422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22050.4869463056123598No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19670.43438702183197814No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT19310.4264368780668784No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19040.42047427024305356No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18740.41384915043880377No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17090.3774109915154299No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16110.3557689334882139No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.299676252478899No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12240.2703048880133916No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA12030.2656673041504167No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11600.2561712990976587No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11520.2544046004831921No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11330.2502086912738339No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11330.2502086912738339No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10980.24247938483554243No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10800.23850431295299257No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC10280.2270207719589596No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9680.21377053235046No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9670.21354969502365168No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9440.20847043650706015No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9440.20847043650706015No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9350.20648290056578525No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9350.20648290056578525No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9330.20604122591216856No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9140.2018453167028104No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9100.2009619673955771No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8900.19654522085941054No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8580.18947842640154408No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8550.1888159144211191No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8500.18771172778707748No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8290.18307414392410262No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7960.17578651213942784No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7950.17556567481261953No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7800.17225311491049464No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7730.17070725362283634No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7530.16629050708666981No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7470.16496548312581985No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7400.16341962183816158No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7390.16319878451135325No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7370.1627571098577366No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7350.16231543520411995No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7140.15767785134114506No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7140.15767785134114506No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.1570153393607201No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7060.15591115272667846No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6990.1543652914390202No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6850.1512735688637036No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6690.1477401716347704No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6680.14751933430796207No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6660.1470776596543454No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6630.14641514767392044No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6480.14310258777179555No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6470.14288175044498722No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6370.14067337717690395No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6350.1402317025232873No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6240.1378024919283957No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6190.1366983052943541No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6140.13559411866031246No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6140.13559411866031246No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6130.1353732813335041No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6110.13493160667988746No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.13493160667988746No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6090.13448993202627083No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6080.13426909469946247No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6070.13404825737265416No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6040.1333857453922292No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG6010.1327232334118042No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5960.13161904677776257No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5880.12985234816329594No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5670.12521476430032108No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5670.12521476430032108No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG5650.12477308964670446No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5600.12366890301266283No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5520.12190220439819621No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5470.12079801776415458No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5330.11770629518883799No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5320.11748545786202967No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5220.11527708459394641No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5210.11505624726713808No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5200.11483540994032976No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5170.11417289795990478No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5160.11395206063309646No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5160.11395206063309646No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5130.11328954865267146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.11306871132586314No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC5110.11284787399905483No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5100.11262703667224648No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5060.1117436873650132No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA5020.11086033805777988No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC4830.10666442884842169No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.10666442884842169No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4810.10622275419480502No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4800.1060019168679967No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4760.10511856756076339No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4720.10423521825353008No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4610.1018060076586385No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4600.10158517033183018No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4590.10136433300502183No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC4550.10048098369778855No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC4540.1002601463709802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG200.001840758337.02
TAGCAAC200.001840758337.01
GTATGAT200.001840758337.01
GTTATCG200.001840758337.011
AGGTCGC200.001840758337.027
ACAACGC451.07751475E-732.888893
AACAACG401.5972328E-632.3752
ACCGTAG303.596064E-430.8333348
GACCGTT1350.030.148157
ACCGGGT250.005492869829.627
GGTCGCT250.005492869829.628
GGGTCTA250.005492869829.637
ACCTGCC250.005492869829.68
GCTCTCG250.005492869829.618
GAGGTCG250.005492869829.626
CTGCCCA250.005492869829.610
TCTCGCG250.005492869829.620
GACTCGG250.005492869829.633
GCGAGGT250.005492869829.624
TAACAAC454.0012073E-628.7777771
Produced by FastQC (version 0.11.5)