FastQCFastQC Report
Fri 10 Feb 2017
ERR1633415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428612
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28320.6607374501880489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.49835282259946057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12060.28137336332160556No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5400.12598807312907712No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5000.11665562326766399No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4990.11642231202112867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.11245602083002808No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4460.10405681595475628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT803.274181E-1127.7513
CATAATA905.456968E-1226.7222232
TGCGGTA358.8602846E-426.4285736
GTCGCCA951.0913936E-1125.3157912
GGTATCA5400.024.3240741
ATAGCGG701.9170693E-723.7857137
GCCGGCA701.9170693E-723.78571315
GTATATC753.7294558E-722.21
GTATCAA13400.021.951491
CGCTCTC603.719271E-521.58333429
ACAACGC450.003821952320.5555573
TTGCGGT450.003821952320.55555735
CCGCTCT759.243555E-619.73333428
CCGGCAG851.241584E-619.58823616
TGCTCGC851.241584E-619.58823610
AATAGCG851.241584E-619.5882366
ATCTCGC902.1458145E-618.511
ATAATAC902.1458145E-618.53
ACTCTGT701.2169349E-418.56
GCTTCGC701.2169349E-418.522