FastQCFastQC Report
Fri 10 Feb 2017
ERR1633414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736557
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36520.49582041851479247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.35489446166420247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13960.18953047761408826No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7880.10698425240680627No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7790.10576235104683004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCG200.001841506637.010
CGCTCGT250.005495078429.59999811
TTACCGG358.8663585E-426.4285731
TGCGGTA601.3363169E-624.66666836
CCGATCT451.3225822E-424.6666649
GGTATCA7500.022.4466691
TAATACG603.7239464E-521.5833344
CGATCTG603.7239464E-521.58333410
GTATCAA19100.021.405761
TAGTACC1450.020.4137944
TACCGCA1500.019.7333347
TATTCCG801.6153856E-518.55
ATAGTAC2200.018.53
TTTACCG500.007032542518.49999830
CGGGATT500.007032542518.49999817
TTTTCGG902.1500819E-618.49999826
CATTCGG1258.563802E-917.7637
CCTACGT1054.791473E-717.6190534
ACGTGAT953.6028287E-617.52631637
TTCGGTC953.6028287E-617.52631628
Produced by FastQC (version 0.11.5)