##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633414.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736557
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.822833806480695	33.0	33.0	33.0	27.0	33.0
2	31.940948222608704	33.0	33.0	33.0	33.0	33.0
3	31.654948632624496	33.0	33.0	33.0	27.0	33.0
4	31.938877778637636	33.0	33.0	33.0	33.0	33.0
5	32.036128907878144	33.0	33.0	33.0	33.0	33.0
6	35.21920638864338	37.0	37.0	37.0	33.0	37.0
7	35.361514451698916	37.0	37.0	37.0	33.0	37.0
8	35.50062656386403	37.0	37.0	37.0	33.0	37.0
9	35.612756378664514	37.0	37.0	37.0	33.0	37.0
10	35.62567051837129	37.0	37.0	37.0	33.0	37.0
11	35.65199841967424	37.0	37.0	37.0	33.0	37.0
12	35.642612859561446	37.0	37.0	37.0	33.0	37.0
13	35.64263458225229	37.0	37.0	37.0	33.0	37.0
14	35.63150441853109	37.0	37.0	37.0	33.0	37.0
15	35.64491546479091	37.0	37.0	37.0	33.0	37.0
16	35.61731950140994	37.0	37.0	37.0	33.0	37.0
17	35.617399603832425	37.0	37.0	37.0	33.0	37.0
18	35.60284675863511	37.0	37.0	37.0	33.0	37.0
19	35.59680513524412	37.0	37.0	37.0	33.0	37.0
20	35.59395403207084	37.0	37.0	37.0	33.0	37.0
21	35.58841474590561	37.0	37.0	37.0	33.0	37.0
22	35.50098906126749	37.0	37.0	37.0	33.0	37.0
23	35.551825588515214	37.0	37.0	37.0	33.0	37.0
24	35.540093977791265	37.0	37.0	37.0	33.0	37.0
25	35.550826344736386	37.0	37.0	37.0	33.0	37.0
26	35.43493850441989	37.0	37.0	37.0	33.0	37.0
27	35.4588524717028	37.0	37.0	37.0	33.0	37.0
28	35.479795861012796	37.0	37.0	37.0	33.0	37.0
29	35.4818703779884	37.0	37.0	37.0	33.0	37.0
30	35.485525220722906	37.0	37.0	37.0	33.0	37.0
31	35.47859025167095	37.0	37.0	37.0	33.0	37.0
32	35.47166342862806	37.0	37.0	37.0	33.0	37.0
33	35.46511267967041	37.0	37.0	37.0	33.0	37.0
34	35.447742673004264	37.0	37.0	37.0	33.0	37.0
35	35.3847713075838	37.0	37.0	37.0	33.0	37.0
36	35.40929351021035	37.0	37.0	37.0	33.0	37.0
37	35.409574547523135	37.0	37.0	37.0	33.0	37.0
38	35.40328854386015	37.0	37.0	37.0	33.0	37.0
39	35.300120696701	37.0	37.0	37.0	33.0	37.0
40	35.09372662265106	37.0	37.0	37.0	33.0	37.0
41	35.25280053003365	37.0	37.0	37.0	33.0	37.0
42	35.299538257052745	37.0	37.0	37.0	33.0	37.0
43	34.95791092882153	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	27.0
16	41.0
17	20.0
18	23.0
19	24.0
20	34.0
21	114.0
22	308.0
23	746.0
24	1460.0
25	2740.0
26	4791.0
27	7131.0
28	10300.0
29	13921.0
30	18149.0
31	23349.0
32	29814.0
33	39483.0
34	56732.0
35	114857.0
36	412490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.878845764822	21.10142188588256	14.103728564116558	23.91600378517888
2	15.77610422547067	21.840536441850393	39.72985118599104	22.6535081466879
3	17.46355000359782	27.737296638277826	30.801010648191518	23.998142709932836
4	12.27304879323664	18.149172433362253	40.02758781737191	29.550190956029198
5	13.27908091295039	37.746705278749644	35.71644828574028	13.257765522559694
6	28.5946640925278	40.33795076280586	17.79088380125367	13.276501343412662
7	25.686946156237738	32.75143675234911	23.981171857710944	17.58044523370221
8	23.86726349759761	36.32359749483068	21.010322351155445	18.79881665641627
9	25.633454030034336	16.080357664104746	20.587001413332572	37.699186892528346
10	14.671505396052172	28.50234265644071	34.17196496673034	22.654186980776778
11	32.65273427582658	24.380190535152067	24.67779139971516	18.28928378930619
12	22.345860537609443	26.42673954629445	30.527033209921296	20.700366706174812
13	27.228035304803296	22.786695394925307	27.05846254940215	22.926806750869247
14	21.85004011909465	21.642045354263146	28.17799572877591	28.329918797866288
15	23.624783961051214	29.02504490487498	25.274622330654655	22.07554880341915
16	22.47918355266463	29.161219023103442	25.469583480979747	22.890013943252185
17	22.00332085636278	28.087303494502123	26.792902653833988	23.11647299530111
18	22.108947440591834	27.850118863848962	28.388026995874043	21.652906699685158
19	24.208038210213196	26.794939156100618	28.460526476565967	20.536496157120222
20	23.950895857347092	26.31703995753214	28.592899123896725	21.13916506122405
21	22.182804589461508	27.658959184421573	27.864781680168676	22.293454545948244
22	21.788130450189193	27.638458394937526	27.96429875759785	22.60911239727543
23	21.95729590513701	27.387832849324628	28.472881256983506	22.181989988554857
24	22.6267620835862	27.903339456416816	27.23428057842095	22.235617881576037
25	22.6807972770607	27.183911089026374	28.19252277827785	21.94276885563507
26	22.600966388208924	27.392177387493433	28.04670921598736	21.960147008310287
27	22.515569059828362	27.303521655486268	27.53513984661065	22.645769438074716
28	21.80713780467771	27.440103074168054	28.487408306485445	22.265350814668793
29	23.26635956212486	27.321850175885913	27.496582070362514	21.915208191626718
30	22.44320534595422	27.49318789991813	28.127354705745788	21.936252048381863
31	22.660432254394433	27.120236451489838	28.072776445000187	22.146554849115546
32	21.45332947755571	27.138157671436154	28.431472377562088	22.97704047344605
33	21.67910969551576	27.061992486664305	28.88235397939331	22.376543838426628
34	22.351970044409324	27.009586495003102	28.177724195140364	22.46071926544721
35	22.42786369554563	27.721004620144807	27.698874628847463	22.1522570554621
36	22.916624239536112	27.37520653527154	27.704848368829566	22.00332085636278
37	22.853356902452898	26.795210689736166	27.581300564654192	22.77013184315674
38	22.780314354489878	27.200338873977163	27.85134076520894	22.168006006324017
39	22.230730276136132	27.147661348680412	28.09694293856416	22.524665436619298
40	22.157416194537557	27.671178198021334	28.254025146729987	21.91738046071112
41	22.0982218619876	26.90613217985845	28.33548523739507	22.66016072075888
42	22.267658850570967	27.363937889396205	27.8373567829781	22.53104647705473
43	22.508916485757382	26.758010581665776	27.947735205829282	22.785337726747557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	43.0
2	55.0
3	201.0
4	347.0
5	347.0
6	440.5
7	534.0
8	510.0
9	486.0
10	662.0
11	838.0
12	838.0
13	1321.0
14	1804.0
15	3579.0
16	5354.0
17	6079.0
18	6804.0
19	6804.0
20	6592.0
21	6380.0
22	7252.5
23	8125.0
24	10191.5
25	12258.0
26	12258.0
27	15088.0
28	17918.0
29	22032.5
30	26147.0
31	29165.5
32	32184.0
33	32184.0
34	36731.0
35	41278.0
36	44086.5
37	46895.0
38	50354.5
39	53814.0
40	53814.0
41	55950.5
42	58087.0
43	57121.0
44	56155.0
45	55429.5
46	54704.0
47	54704.0
48	53366.5
49	52029.0
50	52860.5
51	53692.0
52	52983.5
53	52275.0
54	52275.0
55	47310.0
56	42345.0
57	36516.0
58	30687.0
59	27186.0
60	23685.0
61	23685.0
62	20956.5
63	18228.0
64	14998.0
65	11768.0
66	9789.5
67	7811.0
68	7811.0
69	6469.0
70	5127.0
71	4430.0
72	3733.0
73	2976.5
74	2220.0
75	2220.0
76	1689.0
77	1158.0
78	908.0
79	658.0
80	509.5
81	361.0
82	361.0
83	295.5
84	230.0
85	206.5
86	183.0
87	148.5
88	114.0
89	114.0
90	76.5
91	39.0
92	26.5
93	14.0
94	8.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	736557.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.81326843707661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.61207873914967	46.60879043083077
2	7.435428591271144	8.002494294535769
3	1.954401729491984	3.155182347091167
4	0.8743370384694107	1.882037350225319
5	0.5343900764184111	1.4378638316206915
6	0.37834134488007287	1.2215870611725566
7	0.2751938977133381	1.0366358162925284
8	0.1960062796265775	0.8438190832718172
9	0.1662070947132942	0.8049732303557806
>10	1.2585554893595	13.88249870323856
>50	0.1927364219634588	7.2357652485895985
>100	0.11795460776658931	11.598819044825506
>500	0.003597744027732888	1.2299535796332073
>1k	7.709451487999046E-4	1.0595799783168016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3652	0.49582041851479247	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2614	0.35489446166420247	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1396	0.18953047761408826	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	788	0.10698425240680627	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	779	0.10576235104683004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.715336355502697E-4	0.0	0.0	0.0	0.0
3	2.715336355502697E-4	0.0	0.0	0.0	0.0
4	2.715336355502697E-4	0.0	0.0	0.0	0.0
5	2.715336355502697E-4	0.0	2.715336355502697E-4	0.0	0.0
6	2.715336355502697E-4	0.0	5.430672711005394E-4	0.0	0.0
7	2.715336355502697E-4	0.0	0.0024438027199524272	0.0	0.0
8	2.715336355502697E-4	0.0	0.002579569537727562	0.0	0.0
9	2.715336355502697E-4	0.0	0.002579569537727562	2.715336355502697E-4	0.0
10	4.0730045332540454E-4	0.0	0.003394170444378371	2.715336355502697E-4	0.0
11	4.0730045332540454E-4	0.0	0.005023372257679989	2.715336355502697E-4	0.0
12	5.430672711005394E-4	0.0	0.005566439528780529	6.788340888756743E-4	0.0
13	5.430672711005394E-4	0.0	0.006109506799881068	6.788340888756743E-4	0.0
14	5.430672711005394E-4	0.0	0.006245273617656203	6.788340888756743E-4	0.0
15	5.430672711005394E-4	0.0	0.006788340888756742	6.788340888756743E-4	0.0
16	5.430672711005394E-4	0.0	0.007602941795407552	6.788340888756743E-4	0.0
17	5.430672711005394E-4	0.0	0.0077387086131826864	6.788340888756743E-4	0.0
18	5.430672711005394E-4	0.0	0.007874475430957822	6.788340888756743E-4	0.0
19	5.430672711005394E-4	0.0	0.008553309519833496	6.788340888756743E-4	0.0
20	5.430672711005394E-4	0.0	0.008824843155383766	6.788340888756743E-4	0.0
21	5.430672711005394E-4	0.0	0.009096376790934035	8.146009066508091E-4	1.3576681777513486E-4
22	5.430672711005394E-4	0.0	0.009639444062034574	0.0010861345422010788	1.3576681777513486E-4
23	5.430672711005394E-4	0.0	0.009775210879809709	0.0013576681777513486	1.3576681777513486E-4
24	5.430672711005394E-4	0.0	0.009775210879809709	0.002036502266627023	1.3576681777513486E-4
25	5.430672711005394E-4	0.0	0.009775210879809709	0.0023080359021772925	1.3576681777513486E-4
26	5.430672711005394E-4	0.0	0.010046744515359978	0.0024438027199524272	1.3576681777513486E-4
27	5.430672711005394E-4	0.0	0.010046744515359978	0.003394170444378371	1.3576681777513486E-4
28	5.430672711005394E-4	0.0	0.010046744515359978	0.010861345422010789	1.3576681777513486E-4
29	5.430672711005394E-4	0.0	0.010046744515359978	0.024981094470624812	1.3576681777513486E-4
30	5.430672711005394E-4	0.0	0.010046744515359978	0.0443957494124691	1.3576681777513486E-4
31	5.430672711005394E-4	0.0	0.010046744515359978	0.08974186654936414	1.3576681777513486E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTCG	20	0.0018415066	37.0	10
CGCTCGT	25	0.0054950784	29.599998	11
TTACCGG	35	8.8663585E-4	26.42857	31
TGCGGTA	60	1.3363169E-6	24.666668	36
CCGATCT	45	1.3225822E-4	24.666664	9
GGTATCA	750	0.0	22.446669	1
TAATACG	60	3.7239464E-5	21.583334	4
CGATCTG	60	3.7239464E-5	21.583334	10
GTATCAA	1910	0.0	21.40576	1
TAGTACC	145	0.0	20.413794	4
TACCGCA	150	0.0	19.733334	7
TATTCCG	80	1.6153856E-5	18.5	5
ATAGTAC	220	0.0	18.5	3
TTTACCG	50	0.0070325425	18.499998	30
CGGGATT	50	0.0070325425	18.499998	17
TTTTCGG	90	2.1500819E-6	18.499998	26
CATTCGG	125	8.563802E-9	17.76	37
CCTACGT	105	4.791473E-7	17.61905	34
ACGTGAT	95	3.6028287E-6	17.526316	37
TTCGGTC	95	3.6028287E-6	17.526316	28
>>END_MODULE