FastQCFastQC Report
Fri 10 Feb 2017
ERR1633413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389025
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18080.4647516226463595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.3534477218687745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8240.21181158023263286No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5670.14574898785425103No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4760.12235717498875394No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4710.12107191054559477No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4630.11901548743654007No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4310.11078979500032131No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4170.10719105455947561No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4170.10719105455947561No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT3950.10153589100957523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCGT303.5951397E-430.83333424
CGCCGTA250.005491930529.59999825
TGCGGTA358.858806E-426.42857236
GTATCAA8950.025.0111731
ACTGCGG400.001928903623.12533
CGTTTAG400.001928903623.12526
ACGGTAC400.001928903623.1253
GGTATCA2900.022.3275851
TTGCGGT502.697652E-422.19999935
CGGTACT450.003821326720.5555574
AATACTG1800.020.5555575
AGGTCGC951.6689955E-719.47368435
TCACGGT500.00702672418.4999981
GTCGCCC1002.8627983E-718.49999837
CACGGTA500.00702672418.4999982
ATGCGTT500.00702672418.49999836
CATCGGC500.00702672418.4999989
CCGAATT500.00702672418.49999814
CGCCGTC500.00702672418.49999826
ATCGGAA500.00702672418.49999825
Produced by FastQC (version 0.11.5)