##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633413.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389025
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.856675020885547	33.0	33.0	33.0	27.0	33.0
2	31.240802005012533	33.0	33.0	33.0	27.0	33.0
3	31.011479982006296	33.0	33.0	33.0	27.0	33.0
4	31.339913887282307	33.0	33.0	33.0	27.0	33.0
5	31.47531649636913	33.0	33.0	33.0	27.0	33.0
6	34.096690444058865	37.0	33.0	37.0	27.0	37.0
7	34.288233404022876	37.0	33.0	37.0	27.0	37.0
8	34.41808110018636	37.0	37.0	37.0	27.0	37.0
9	34.53675727780991	37.0	37.0	37.0	27.0	37.0
10	34.47154295996401	37.0	37.0	37.0	27.0	37.0
11	34.521478054109636	37.0	37.0	37.0	27.0	37.0
12	34.58010153589101	37.0	37.0	37.0	27.0	37.0
13	34.63438853544117	37.0	37.0	37.0	27.0	37.0
14	34.59604909710173	37.0	37.0	37.0	27.0	37.0
15	34.63866846603689	37.0	37.0	37.0	27.0	37.0
16	34.59362508836193	37.0	37.0	37.0	27.0	37.0
17	34.6027427543217	37.0	37.0	37.0	27.0	37.0
18	34.59007775849881	37.0	37.0	37.0	27.0	37.0
19	34.56775785617891	37.0	37.0	37.0	27.0	37.0
20	34.565629458261036	37.0	37.0	37.0	27.0	37.0
21	34.58676691729323	37.0	37.0	37.0	27.0	37.0
22	34.463561467771996	37.0	37.0	37.0	27.0	37.0
23	34.50825268298953	37.0	37.0	37.0	27.0	37.0
24	34.50056166056166	37.0	37.0	37.0	27.0	37.0
25	34.51921341816079	37.0	37.0	37.0	27.0	37.0
26	34.38847888953152	37.0	37.0	37.0	27.0	37.0
27	34.39809780862412	37.0	37.0	37.0	27.0	37.0
28	34.42912923333976	37.0	37.0	37.0	27.0	37.0
29	34.43373562110404	37.0	37.0	37.0	27.0	37.0
30	34.4117987275882	37.0	37.0	37.0	27.0	37.0
31	34.38981299402352	37.0	37.0	37.0	27.0	37.0
32	34.41239251976094	37.0	37.0	37.0	27.0	37.0
33	34.38227363279995	37.0	37.0	37.0	27.0	37.0
34	34.34932202300623	37.0	37.0	37.0	27.0	37.0
35	34.291567379988436	37.0	37.0	37.0	27.0	37.0
36	34.3223674571043	37.0	37.0	37.0	27.0	37.0
37	34.34071589229484	37.0	37.0	37.0	27.0	37.0
38	34.30140479403637	37.0	37.0	37.0	27.0	37.0
39	34.22102949681897	37.0	37.0	37.0	27.0	37.0
40	33.995514427093376	37.0	33.0	37.0	27.0	37.0
41	34.18904954694428	37.0	37.0	37.0	27.0	37.0
42	34.20613842298053	37.0	37.0	37.0	27.0	37.0
43	33.76923848081743	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	20.0
16	26.0
17	23.0
18	29.0
19	55.0
20	94.0
21	326.0
22	669.0
23	1348.0
24	2371.0
25	3843.0
26	5511.0
27	7599.0
28	9954.0
29	12693.0
30	15528.0
31	19603.0
32	24367.0
33	31220.0
34	44612.0
35	78413.0
36	130719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.135209819420346	22.324529271897692	13.66313218944798	23.877128719233983
2	16.35241951031425	22.75946275946276	37.46211683053788	23.42600089968511
3	17.773150825782405	28.05475226527858	30.323501060343165	23.84859584859585
4	12.773215089004564	19.290791080264764	39.66351776878093	28.272476061949746
5	13.982905982905983	37.61711972238288	35.48203842940685	12.917935865304287
6	28.297410192147034	41.346957136430824	17.31302615513142	13.042606516290729
7	25.92378381852066	32.970631707473814	23.737806053595527	17.367778420409998
8	24.133667502088553	36.33288349077823	20.552406657669817	18.981042349463404
9	25.323565323565322	15.830345093502988	20.984769616348565	37.861319966583125
10	14.751494119915174	28.706638390848916	33.861319966583125	22.680547522652788
11	33.17935865304286	24.1146455883298	24.780155516997624	17.925840241629714
12	22.558704453441294	26.83040935672515	30.24021592442645	20.370670265407107
13	27.370220422852004	23.0044341623289	26.422466422466425	23.202878992352677
14	21.49681897050318	21.762868710237132	28.367071524966263	28.373240794293427
15	23.439624702782595	29.447978921663132	24.936957779063043	22.17543859649123
16	22.33095559411349	29.398624767045824	25.453634085213032	22.816785553627657
17	21.765182186234817	28.52515905147484	26.901355953987533	22.80830280830281
18	22.3972752393805	28.006426322215795	28.347792558318872	21.248505880084828
19	24.253197095302358	26.96664738770002	27.994344836450097	20.785810680547524
20	23.37998843262001	26.92166313218945	28.5490649701176	21.149283465072937
21	22.312447786131997	27.65709144656513	28.40074545337703	21.62971531392584
22	22.036887089518668	27.552727973780605	28.08688387635756	22.323501060343165
23	22.015037593984964	27.311612364243942	28.697898592635436	21.97545144913566
24	22.28108733371891	28.105391684339054	27.07255317781633	22.5409678041257
25	22.513977250819355	27.60259623417518	28.253968253968253	21.629458261037207
26	22.437889595784334	27.85425101214575	28.150375939849624	21.557483452220293
27	22.28931302615513	27.581774950196003	27.656834393676498	22.472077629972368
28	21.78394704710494	27.493091703618017	28.715892294839662	22.007068954437376
29	23.43165606323501	27.220358588779643	27.45401966454598	21.89396568343937
30	22.589036694299853	27.050703682282627	28.44213096844676	21.91812865497076
31	22.44046012467065	27.261744103849367	28.250883619304673	22.04691215217531
32	21.464173253646937	27.167919799498748	28.54212454212454	22.82578240472977
33	21.4590321958743	27.38538654328128	28.985540775014456	22.170040485829958
34	22.48235974551764	27.072039072039072	28.376839534734273	22.068761647709014
35	22.26951995373048	27.893837156995048	27.855793329477542	21.980849559796926
36	23.40055266371056	27.36430820641347	27.4049225628173	21.83021656705867
37	22.504209241051345	26.887218045112782	27.898978214767688	22.709594499068185
38	22.482616798406273	27.344258081100186	27.839856050382366	22.333269070111175
39	21.82610372084056	27.407493091703618	28.19613135402609	22.57027183342973
40	22.2317331791016	27.862990810359236	28.262708052181733	21.642567958357432
41	22.006554848660112	27.014459224985544	28.23520339309813	22.74378253325622
42	21.87340145234882	27.715442452284556	28.168369642053854	22.242786453312767
43	22.618854829381146	26.67155067155067	27.894351262772314	22.815243236295867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	18.0
2	20.0
3	90.5
4	161.0
5	161.0
6	201.0
7	241.0
8	227.0
9	213.0
10	316.5
11	420.0
12	420.0
13	691.5
14	963.0
15	1813.5
16	2664.0
17	3046.5
18	3429.0
19	3429.0
20	3487.0
21	3545.0
22	3817.5
23	4090.0
24	5217.5
25	6345.0
26	6345.0
27	8131.5
28	9918.0
29	11305.0
30	12692.0
31	14754.0
32	16816.0
33	16816.0
34	20048.0
35	23280.0
36	24912.0
37	26544.0
38	27898.5
39	29253.0
40	29253.0
41	30138.5
42	31024.0
43	31404.5
44	31785.0
45	30799.0
46	29813.0
47	29813.0
48	28454.5
49	27096.0
50	26917.0
51	26738.0
52	25239.5
53	23741.0
54	23741.0
55	22884.0
56	22027.0
57	18723.5
58	15420.0
59	14375.5
60	13331.0
61	13331.0
62	11618.0
63	9905.0
64	8107.5
65	6310.0
66	5305.0
67	4300.0
68	4300.0
69	3545.0
70	2790.0
71	2286.5
72	1783.0
73	1399.0
74	1015.0
75	1015.0
76	761.5
77	508.0
78	415.0
79	322.0
80	253.5
81	185.0
82	185.0
83	150.0
84	115.0
85	108.0
86	101.0
87	82.5
88	64.0
89	64.0
90	49.5
91	35.0
92	21.0
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	389025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.05531282499263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93743704941781	52.21054978205891
2	6.969883387259541	8.371570543511822
3	2.0273189646380723	3.6525382385213883
4	1.0045985063965035	2.4132591026064953
5	0.6291604359244543	1.8892213398275917
6	0.4062671767560693	1.4639101434367392
7	0.3066306665038641	1.2890360419037885
8	0.2230322201219285	1.0715415799580026
9	0.1712339557873074	0.9255157902960933
>10	1.1414236671724916	13.404157735690625
>50	0.11709424622262095	4.95114153581697
>100	0.06418499422567583	7.16626298568712
>500	8.673647868334571E-4	0.3622850013843643
>1k	8.673647868334571E-4	0.8290101793000946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1808	0.4647516226463595	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.3534477218687745	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	824	0.21181158023263286	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	567	0.14574898785425103	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	476	0.12235717498875394	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	471	0.12107191054559477	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	463	0.11901548743654007	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	431	0.11078979500032131	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	417	0.10719105455947561	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	417	0.10719105455947561	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	395	0.10153589100957523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.57052888631836E-4	0.0	0.0
2	0.0	0.0	2.57052888631836E-4	0.0	0.0
3	0.0	0.0	5.14105777263672E-4	0.0	0.0
4	0.0	0.0	5.14105777263672E-4	2.57052888631836E-4	0.0
5	0.0	0.0	0.001028211554527344	2.57052888631836E-4	0.0
6	0.0	0.0	0.001028211554527344	2.57052888631836E-4	0.0
7	0.0	0.0	0.001542317331791016	2.57052888631836E-4	0.0
8	0.0	0.0	0.001542317331791016	2.57052888631836E-4	0.0
9	0.0	0.0	0.002313475997686524	7.71158665895508E-4	0.0
10	2.57052888631836E-4	0.0	0.002313475997686524	7.71158665895508E-4	0.0
11	2.57052888631836E-4	0.0	0.003341687552213868	0.00128526444315918	0.0
12	2.57052888631836E-4	0.0	0.003341687552213868	0.00128526444315918	0.0
13	2.57052888631836E-4	0.0	0.00385579332947754	0.00128526444315918	0.0
14	2.57052888631836E-4	0.0	0.00385579332947754	0.001542317331791016	0.0
15	2.57052888631836E-4	0.0	0.00385579332947754	0.001542317331791016	0.0
16	2.57052888631836E-4	0.0	0.004112846218109376	0.001542317331791016	0.0
17	2.57052888631836E-4	0.0	0.004112846218109376	0.001542317331791016	0.0
18	2.57052888631836E-4	0.0	0.004369899106741212	0.001542317331791016	0.0
19	2.57052888631836E-4	0.0	0.004626951995373048	0.001542317331791016	0.0
20	2.57052888631836E-4	0.0	0.004884004884004884	0.001799370220422852	0.0
21	2.57052888631836E-4	0.0	0.004884004884004884	0.002056423109054688	0.0
22	2.57052888631836E-4	0.0	0.004884004884004884	0.00257052888631836	0.0
23	2.57052888631836E-4	0.0	0.004884004884004884	0.003084634663582032	0.0
24	2.57052888631836E-4	0.0	0.004884004884004884	0.004112846218109376	0.0
25	2.57052888631836E-4	0.0	0.004884004884004884	0.004626951995373048	0.0
26	2.57052888631836E-4	0.0	0.004884004884004884	0.004626951995373048	0.0
27	2.57052888631836E-4	0.0	0.004884004884004884	0.0064263222157959	0.0
28	2.57052888631836E-4	0.0	0.004884004884004884	0.010539168433905276	0.0
29	2.57052888631836E-4	0.0	0.004884004884004884	0.02056423109054688	0.0
30	2.57052888631836E-4	0.0	0.004884004884004884	0.03727266885161622	0.0
31	2.57052888631836E-4	0.0	0.004884004884004884	0.07531649636912795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCCGT	30	3.5951397E-4	30.833334	24
CGCCGTA	25	0.0054919305	29.599998	25
TGCGGTA	35	8.858806E-4	26.428572	36
GTATCAA	895	0.0	25.011173	1
ACTGCGG	40	0.0019289036	23.125	33
CGTTTAG	40	0.0019289036	23.125	26
ACGGTAC	40	0.0019289036	23.125	3
GGTATCA	290	0.0	22.327585	1
TTGCGGT	50	2.697652E-4	22.199999	35
CGGTACT	45	0.0038213267	20.555557	4
AATACTG	180	0.0	20.555557	5
AGGTCGC	95	1.6689955E-7	19.473684	35
TCACGGT	50	0.007026724	18.499998	1
GTCGCCC	100	2.8627983E-7	18.499998	37
CACGGTA	50	0.007026724	18.499998	2
ATGCGTT	50	0.007026724	18.499998	36
CATCGGC	50	0.007026724	18.499998	9
CCGAATT	50	0.007026724	18.499998	14
CGCCGTC	50	0.007026724	18.499998	26
ATCGGAA	50	0.007026724	18.499998	25
>>END_MODULE