FastQCFastQC Report
Fri 10 Feb 2017
ERR1633412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650444
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36620.5630000430475183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.41079631759229085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14200.21831241428931625No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8010.12314665059559317No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7980.12268542718512278No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA7370.11330721783889158No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7290.1120772887443039No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6940.10669634895548272No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6590.10131540916666153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.10100792689301463No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6540.10054670348254424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATG451.3223494E-424.66666611
GTATCAA17550.024.6666661
CCTTGCG451.3223494E-424.66666633
GGTATCA5850.023.7179491
CGTAGGC400.001930291323.12518
GTATACG400.001930291323.1251
ATGCCCG1155.456968E-1222.5217429
CCGGCAG502.7003555E-422.19999916
CTTGCGG502.7003555E-422.19999934
GCCGGCA450.00382404920.55555515
AAACGCA450.00382404920.5555555
TTCGCCG450.00382404920.55555524
TGCGGTA701.2181648E-418.536
AACGCAC609.2309405E-418.532
TGCACCG609.2309405E-418.55
ATTCCGG500.0070316818.4999981
TATCGGA500.0070316818.49999826
CGAACTA2450.018.12244829
AACGGCC1950.018.02564237
TTAACGG1900.017.52631635