FastQCFastQC Report
Fri 10 Feb 2017
ERR1633411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695474
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48510.6975098997230667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.4838426742049307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19000.27319497206221943No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9520.13688505968591205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.11675490384974851No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7750.1114347912359053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.025.5988371
CGCCGTC601.3361387E-624.66666826
GCCGTCC601.3361387E-624.66666827
GTATCAA23950.023.7912331
GGTTGCC400.001930424123.12500237
TCCGCCG652.6803173E-622.7692331
CCGTCCG652.6803173E-622.7692328
CGCATAC502.700616E-422.19999924
CGTCACG502.700616E-422.19999918
CGTCCGC705.097143E-621.14285729
GTCTAAT555.140287E-420.1818181
CGTAATT555.140287E-420.18181816
ACGTTAG656.897123E-519.9230771
GCGCATA656.897123E-519.92307723
CGAGTCG759.2571445E-619.73333421
GTCCGGC851.2438613E-619.58823615
CAAACGT701.21831894E-418.514
ACGTAAT609.231815E-418.515
CGTTTAG609.231815E-418.526
TGTCCGG902.149729E-618.514
Produced by FastQC (version 0.11.5)