##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633411.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695474
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757932000333586	33.0	33.0	33.0	27.0	33.0
2	31.89874675401237	33.0	33.0	33.0	33.0	33.0
3	31.63019322073866	33.0	33.0	33.0	27.0	33.0
4	31.90471678308607	33.0	33.0	33.0	33.0	33.0
5	32.0075631871213	33.0	33.0	33.0	33.0	33.0
6	35.15987082191426	37.0	37.0	37.0	33.0	37.0
7	35.31509445356692	37.0	37.0	37.0	33.0	37.0
8	35.45121169159451	37.0	37.0	37.0	33.0	37.0
9	35.556568613636166	37.0	37.0	37.0	33.0	37.0
10	35.571876159281295	37.0	37.0	37.0	33.0	37.0
11	35.58578034549099	37.0	37.0	37.0	33.0	37.0
12	35.58777323091877	37.0	37.0	37.0	33.0	37.0
13	35.56697590420347	37.0	37.0	37.0	33.0	37.0
14	35.56222950103095	37.0	37.0	37.0	33.0	37.0
15	35.57933581988687	37.0	37.0	37.0	33.0	37.0
16	35.561049011177985	37.0	37.0	37.0	33.0	37.0
17	35.55472958011371	37.0	37.0	37.0	33.0	37.0
18	35.52679179954966	37.0	37.0	37.0	33.0	37.0
19	35.52336679732096	37.0	37.0	37.0	33.0	37.0
20	35.52513825103455	37.0	37.0	37.0	33.0	37.0
21	35.519750558611825	37.0	37.0	37.0	33.0	37.0
22	35.421045215205744	37.0	37.0	37.0	33.0	37.0
23	35.46631074633991	37.0	37.0	37.0	33.0	37.0
24	35.46047443901569	37.0	37.0	37.0	33.0	37.0
25	35.48024225204681	37.0	37.0	37.0	33.0	37.0
26	35.37748930944938	37.0	37.0	37.0	33.0	37.0
27	35.38697924005786	37.0	37.0	37.0	33.0	37.0
28	35.39723411658811	37.0	37.0	37.0	33.0	37.0
29	35.408338773268305	37.0	37.0	37.0	33.0	37.0
30	35.39731895081628	37.0	37.0	37.0	33.0	37.0
31	35.40009403658512	37.0	37.0	37.0	33.0	37.0
32	35.39170407520626	37.0	37.0	37.0	33.0	37.0
33	35.383557113565715	37.0	37.0	37.0	33.0	37.0
34	35.35264006993791	37.0	37.0	37.0	33.0	37.0
35	35.29238332417891	37.0	37.0	37.0	33.0	37.0
36	35.321185263575636	37.0	37.0	37.0	33.0	37.0
37	35.322081055510345	37.0	37.0	37.0	33.0	37.0
38	35.30379568466974	37.0	37.0	37.0	33.0	37.0
39	35.19741212468043	37.0	37.0	37.0	33.0	37.0
40	34.97355472670438	37.0	37.0	37.0	27.0	37.0
41	35.14431883866255	37.0	37.0	37.0	33.0	37.0
42	35.17901316224618	37.0	37.0	37.0	33.0	37.0
43	34.82025640067062	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	17.0
16	33.0
17	39.0
18	23.0
19	31.0
20	33.0
21	135.0
22	353.0
23	862.0
24	1622.0
25	3009.0
26	4996.0
27	7534.0
28	10485.0
29	13871.0
30	17822.0
31	22780.0
32	28978.0
33	38233.0
34	54707.0
35	109460.0
36	380449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.11095454323239	21.023934755289197	14.569056499595959	23.29605420188246
2	15.435515921515396	22.745638226590785	40.00566520099961	21.81318065089421
3	17.569024866493933	28.213707485829808	31.347110028556063	22.870157619120196
4	12.052068085938512	18.585885309874993	40.828701001043896	28.533345603142607
5	13.199630755427233	37.60313110195349	35.51505879443372	13.682179348185555
6	27.350698947768	41.33094838915618	18.013613736818343	13.304738926257487
7	24.69855091635345	33.61247149426147	23.926847013691383	17.7621305756937
8	23.81915067996791	35.82980815961488	21.403675766455684	18.94736539396153
9	25.811317173611094	15.770970589842323	20.910774522124477	37.50693771442211
10	14.501045330235208	28.629251417019187	34.1699330240958	22.69977022864981
11	32.48043780213207	24.90028383519729	24.800927137463084	17.818351225207557
12	22.485240282167272	26.566198017467222	31.16033093976195	19.788230760603557
13	27.897232678719835	23.27031635977765	26.985624192996433	21.846826768506084
14	21.92015805048068	22.175667242772555	29.106048536681456	26.798126170065306
15	23.331281974595743	29.224241308805215	25.859054400308278	21.58542231629076
16	21.57463830423567	29.65660829880053	26.057767795776694	22.710985601187105
17	21.561697489769568	28.016575745462806	27.287720317366286	23.13400644740134
18	22.490416607953712	27.12725421798658	29.13983844112073	21.242490732938972
19	24.29134662115334	26.543479698737837	29.184843718097298	19.98032996201152
20	24.200185772581	26.156405559373898	28.986417896283683	20.656990771761418
21	22.4028504300664	27.543517083312963	28.57806330646437	21.475569180156267
22	21.747182497117073	27.477375142708425	28.810135245889857	21.965307114284645
23	21.76860673439985	27.15658673077642	29.295847148851	21.778959385972733
24	22.07659811869315	27.373561053324785	28.610990489939237	21.93885033804283
25	22.214345899343467	27.213526314427284	29.120714793076374	21.45141299315287
26	22.23763936538246	27.428200047737228	28.675982135924567	21.658178450955752
27	22.33239488463983	27.364214909543705	28.4718048410149	21.83158536480156
28	21.59131757621421	27.608508729298293	28.977790686639615	21.822383007847883
29	22.50493907752123	27.40763853141886	28.350017398206116	21.737404992853797
30	22.137563733511247	27.396998306191172	29.053566344680032	21.41187161561755
31	22.458639719098052	27.107411635805224	28.718974397317513	21.71497424777921
32	21.14859793464601	27.22057186896994	29.117551482873548	22.5132787135105
33	21.58901698697579	27.182036999226426	29.550493620178468	21.678452393619317
34	21.739993155747015	27.02128332619192	28.92401441319158	22.314709104869486
35	21.885217851422194	27.766674239439578	28.67166853110253	21.6764393780357
36	22.241809183377093	27.34480368784455	28.71451700566808	21.698870123110282
37	22.510834337444678	27.01279990337525	28.340671254425036	22.13569450475503
38	22.32764991933559	27.321941582287764	28.514653315580453	21.835755182796195
39	22.016639011666864	27.142064261208905	28.741261355564696	22.10003537155954
40	21.876015494468522	27.577019414097435	28.732346572265822	21.81461851916822
41	22.043814722045685	26.970239002464506	28.81358612974748	22.172360145742328
42	21.782122696175556	27.2775114526208	28.850826918044387	22.089538933159254
43	21.973502963446514	26.986055553478632	28.73852940584407	22.30191207723078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	60.5
2	75.0
3	266.5
4	458.0
5	458.0
6	576.0
7	694.0
8	627.5
9	561.0
10	807.0
11	1053.0
12	1053.0
13	1665.0
14	2277.0
15	4441.5
16	6606.0
17	7652.5
18	8699.0
19	8699.0
20	8456.0
21	8213.0
22	9111.0
23	10009.0
24	12569.0
25	15129.0
26	15129.0
27	18045.0
28	20961.0
29	24719.0
30	28477.0
31	30901.5
32	33326.0
33	33326.0
34	36363.0
35	39400.0
36	41076.5
37	42753.0
38	44369.5
39	45986.0
40	45986.0
41	47765.0
42	49544.0
43	48835.0
44	48126.0
45	48275.0
46	48424.0
47	48424.0
48	48123.0
49	47822.0
50	47900.5
51	47979.0
52	47963.0
53	47947.0
54	47947.0
55	43840.0
56	39733.0
57	35606.5
58	31480.0
59	27490.0
60	23500.0
61	23500.0
62	20432.0
63	17364.0
64	13806.5
65	10249.0
66	8576.0
67	6903.0
68	6903.0
69	5641.0
70	4379.0
71	3750.0
72	3121.0
73	2526.5
74	1932.0
75	1932.0
76	1400.0
77	868.0
78	669.5
79	471.0
80	376.5
81	282.0
82	282.0
83	245.0
84	208.0
85	206.5
86	205.0
87	166.5
88	128.0
89	128.0
90	92.5
91	57.0
92	41.0
93	25.0
94	14.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	695474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.25838698858072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.32498119610858	54.10645878870335
2	6.9584725131820955	8.525296041238214
3	1.6339271939243827	3.002752330697569
4	0.6934813945207772	1.6992620653973753
5	0.43437076575742645	1.3304426232647288
6	0.28700537455359815	1.0548891781324123
7	0.19359233431795717	0.8301407893570482
8	0.16244515100200385	0.7960902339599346
9	0.12569177046537083	0.6929707604802792
>10	0.9818536918720091	12.451198739495922
>50	0.13064585028995296	5.426398621051314
>100	0.07115306290544438	7.893805647975621
>500	0.0016657907703495139	0.7156175794229195
>1k	7.139103301497917E-4	1.474676600823437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4851	0.6975098997230667	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3365	0.4838426742049307	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1900	0.27319497206221943	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	952	0.13688505968591205	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	812	0.11675490384974851	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	775	0.1114347912359053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.4378682740116814E-4	0.0	0.0
2	0.0	0.0	1.4378682740116814E-4	0.0	0.0
3	0.0	0.0	4.313604822035044E-4	0.0	0.0
4	0.0	0.0	4.313604822035044E-4	0.0	0.0
5	0.0	0.0	5.751473096046725E-4	0.0	0.0
6	0.0	0.0	8.627209644070088E-4	0.0	0.0
7	0.0	0.0	0.0014378682740116813	0.0	0.0
8	0.0	0.0	0.0015816551014128494	0.0	0.0
9	0.0	0.0	0.002156802411017522	1.4378682740116814E-4	0.0
10	1.4378682740116814E-4	0.0	0.0025881628932210264	2.875736548023363E-4	0.0
11	1.4378682740116814E-4	0.0	0.004313604822035044	5.751473096046725E-4	0.0
12	1.4378682740116814E-4	0.0	0.004313604822035044	7.189341370058407E-4	0.0
13	1.4378682740116814E-4	0.0	0.005176325786442053	7.189341370058407E-4	0.0
14	1.4378682740116814E-4	0.0	0.005607686268645557	8.627209644070088E-4	0.0
15	1.4378682740116814E-4	0.0	0.006326620405651398	8.627209644070088E-4	0.0
16	1.4378682740116814E-4	0.0	0.0066141940604537335	0.0010065077918081768	0.0
17	1.4378682740116814E-4	0.0	0.0066141940604537335	0.0010065077918081768	0.0
18	1.4378682740116814E-4	0.0	0.00690176771525607	0.0010065077918081768	0.0
19	2.875736548023363E-4	0.0	0.0070455545426572385	0.0010065077918081768	0.0
20	2.875736548023363E-4	0.0	0.007189341370058406	0.001150294619209345	0.0
21	2.875736548023363E-4	0.0	0.007476915024860743	0.0012940814466105132	0.0
22	2.875736548023363E-4	0.0	0.007620701852261911	0.0015816551014128494	0.0
23	2.875736548023363E-4	0.0	0.007908275507064248	0.0020130155836163535	0.0
24	2.875736548023363E-4	0.0	0.008052062334465414	0.0027319497206221943	0.0
25	4.313604822035044E-4	1.4378682740116814E-4	0.008052062334465414	0.0028757365480233626	0.0
26	4.313604822035044E-4	1.4378682740116814E-4	0.008052062334465414	0.004169817994633875	0.0
27	7.189341370058407E-4	1.4378682740116814E-4	0.008052062334465414	0.005463899441244389	0.0
28	7.189341370058407E-4	1.4378682740116814E-4	0.008052062334465414	0.013228388120907467	0.0
29	7.189341370058407E-4	1.4378682740116814E-4	0.008052062334465414	0.031057954718652313	0.0
30	7.189341370058407E-4	1.4378682740116814E-4	0.008052062334465414	0.0555017153768509	0.0
31	7.189341370058407E-4	1.4378682740116814E-4	0.008052062334465414	0.11459810143873099	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	860	0.0	25.598837	1
CGCCGTC	60	1.3361387E-6	24.666668	26
GCCGTCC	60	1.3361387E-6	24.666668	27
GTATCAA	2395	0.0	23.791233	1
GGTTGCC	40	0.0019304241	23.125002	37
TCCGCCG	65	2.6803173E-6	22.76923	31
CCGTCCG	65	2.6803173E-6	22.76923	28
CGCATAC	50	2.700616E-4	22.199999	24
CGTCACG	50	2.700616E-4	22.199999	18
CGTCCGC	70	5.097143E-6	21.142857	29
GTCTAAT	55	5.140287E-4	20.181818	1
CGTAATT	55	5.140287E-4	20.181818	16
ACGTTAG	65	6.897123E-5	19.923077	1
GCGCATA	65	6.897123E-5	19.923077	23
CGAGTCG	75	9.2571445E-6	19.733334	21
GTCCGGC	85	1.2438613E-6	19.588236	15
CAAACGT	70	1.21831894E-4	18.5	14
ACGTAAT	60	9.231815E-4	18.5	15
CGTTTAG	60	9.231815E-4	18.5	26
TGTCCGG	90	2.149729E-6	18.5	14
>>END_MODULE