FastQCFastQC Report
Fri 10 Feb 2017
ERR1633410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755681
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59240.7839286683137461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.5391163731786296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22540.29827400715381225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.13484525877982906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT405.937479E-527.757
GGTATCA9850.027.4213181
TTAACGG601.3363933E-624.66666635
TTCGCCG751.3722456E-824.66666624
AACGGCC551.9015404E-523.54545437
GCCGCTC903.8235157E-922.6111127
GCCGGCA1005.329639E-1022.19999915
GTATCAA24200.022.0929761
GGACCGT705.0981052E-621.1428596
ACGGTAC450.003824613520.5555553
AACCACG555.1408523E-420.18181835
CGAGTCG701.2184958E-418.521
TAACGGC701.2184958E-418.536
CCGTCCG609.2328247E-418.528
GACGTAT500.007032708318.49999828
GGACGTA500.007032708318.49999827
AAACACG500.007032708318.49999815
CCGTTTA500.007032708318.49999827
TTGCGGT852.7211167E-517.41176435
CACGTAG852.7211167E-517.41176426
Produced by FastQC (version 0.11.5)