##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633410.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	755681
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910994189347093	33.0	33.0	33.0	33.0	33.0
2	32.015625640978136	33.0	33.0	33.0	33.0	33.0
3	31.73564374385488	33.0	33.0	33.0	27.0	33.0
4	32.00871664101651	33.0	33.0	33.0	33.0	33.0
5	32.10574700171104	33.0	33.0	33.0	33.0	33.0
6	35.287774867966775	37.0	37.0	37.0	33.0	37.0
7	35.432642874440404	37.0	37.0	37.0	33.0	37.0
8	35.563826535270834	37.0	37.0	37.0	33.0	37.0
9	35.67136000508151	37.0	37.0	37.0	33.0	37.0
10	35.6770621465936	37.0	37.0	37.0	33.0	37.0
11	35.702387647697904	37.0	37.0	37.0	33.0	37.0
12	35.69352544261401	37.0	37.0	37.0	33.0	37.0
13	35.6799932775867	37.0	37.0	37.0	33.0	37.0
14	35.673759165573834	37.0	37.0	37.0	33.0	37.0
15	35.69682445370467	37.0	37.0	37.0	33.0	37.0
16	35.67720374073187	37.0	37.0	37.0	33.0	37.0
17	35.67622052162222	37.0	37.0	37.0	33.0	37.0
18	35.66760312883346	37.0	37.0	37.0	33.0	37.0
19	35.65924113481747	37.0	37.0	37.0	33.0	37.0
20	35.65885340507436	37.0	37.0	37.0	33.0	37.0
21	35.65785562955797	37.0	37.0	37.0	33.0	37.0
22	35.56974172964518	37.0	37.0	37.0	33.0	37.0
23	35.60372564613905	37.0	37.0	37.0	33.0	37.0
24	35.6045937373045	37.0	37.0	37.0	33.0	37.0
25	35.623105516745824	37.0	37.0	37.0	33.0	37.0
26	35.50725372213937	37.0	37.0	37.0	33.0	37.0
27	35.52072633823002	37.0	37.0	37.0	33.0	37.0
28	35.54139511248794	37.0	37.0	37.0	33.0	37.0
29	35.54548149285214	37.0	37.0	37.0	33.0	37.0
30	35.5489723838498	37.0	37.0	37.0	33.0	37.0
31	35.53846265818513	37.0	37.0	37.0	33.0	37.0
32	35.53271552414312	37.0	37.0	37.0	33.0	37.0
33	35.52502047821766	37.0	37.0	37.0	33.0	37.0
34	35.5037178386118	37.0	37.0	37.0	33.0	37.0
35	35.43795728620939	37.0	37.0	37.0	33.0	37.0
36	35.46610143698201	37.0	37.0	37.0	33.0	37.0
37	35.453688791963806	37.0	37.0	37.0	33.0	37.0
38	35.44681949129328	37.0	37.0	37.0	33.0	37.0
39	35.33005328968176	37.0	37.0	37.0	33.0	37.0
40	35.13563527467278	37.0	37.0	37.0	33.0	37.0
41	35.288611199699346	37.0	37.0	37.0	33.0	37.0
42	35.322200769901585	37.0	37.0	37.0	33.0	37.0
43	34.99074477193419	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	23.0
16	39.0
17	33.0
18	34.0
19	21.0
20	49.0
21	126.0
22	285.0
23	733.0
24	1395.0
25	2776.0
26	4509.0
27	6982.0
28	9990.0
29	13330.0
30	17898.0
31	22990.0
32	29654.0
33	39137.0
34	56654.0
35	115015.0
36	434004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64958494391151	20.74870216400836	14.385302793109792	23.216410098970332
2	15.573767237763025	22.486340135586314	39.54168491731299	22.39820770933767
3	17.682328919213266	27.87670988155055	31.074620110866885	23.3663410883693
4	11.915080569711293	18.307460423115042	40.95934660260083	28.818112404572826
5	13.247521110098043	37.45733980343557	35.32747283576006	13.967666250706317
6	27.919849777882465	41.145933270784894	17.656524379996323	13.277692571336319
7	25.21315211048048	33.36010829966613	23.73250088330923	17.694238706544162
8	24.41387304960691	35.39747591907167	21.195186857946673	18.993464173374743
9	26.138013262209846	15.551535634745347	20.880636141440633	37.429814961604166
10	14.61899928673607	28.72534839436217	33.90544422845089	22.750208090450865
11	32.770838488727385	24.721145562744066	24.804514074060354	17.703501874468195
12	22.83092998236028	26.35781500395008	31.07276747728208	19.738487536407558
13	28.305329894492516	23.074815960702995	26.436551931304347	22.18330221350014
14	21.865972546616895	21.831037170446262	29.18162558010589	27.12136470283096
15	23.179622089214895	29.45409504804276	25.534451706474027	21.83183115626832
16	21.570212827899603	29.371520522548533	26.13655762153607	22.92170902801579
17	21.594297064502086	27.856198581147336	27.42413796297644	23.12536639137414
18	22.813726956215653	26.94867278653294	28.952560670441628	21.285039586809777
19	24.312772188264624	26.534344518388046	28.99517124289217	20.15771205045515
20	24.175147979107585	26.439727874592585	28.599104648654656	20.78601949764517
21	22.785275797591844	27.154182783476095	28.665667126737336	21.39487429219472
22	21.956354599361372	27.291013006811077	29.133457106900927	21.619175286926627
23	22.04329604687692	26.724504122771382	29.33645281540756	21.895747014944135
24	21.924198173567948	26.932528408151057	29.038840463105466	22.10443295517553
25	22.323970034975073	27.129569222992238	29.136236057278136	21.410224684754546
26	22.355067813005753	27.034423255315403	28.85992899120131	21.75057994047753
27	22.283476758050025	27.111572211025553	28.87223577144324	21.732715259481182
28	22.006905030032513	27.08510601695689	28.933637341682534	21.97435161132806
29	22.34580464508172	27.18792718091364	28.743345406328864	21.72292276767578
30	22.44756716127572	27.09661881137676	28.867339525540537	21.588474501806978
31	22.274081259155647	27.205791861909983	28.81612744001768	21.703999438916686
32	21.46712700200217	27.09992708563534	29.102756321781282	22.330189590581213
33	21.48485935202817	26.996576597797215	29.583514737038513	21.935049313136098
34	22.00875766361732	26.746206401907685	29.05670514410181	22.188330790373186
35	22.00836067070629	27.221406916410494	28.83902069788707	21.93121171499614
36	22.246556417324243	27.14584593234447	28.904392197236668	21.703205453094625
37	22.52259882146038	26.79040494600235	28.815333454195617	21.871662778341655
38	22.41223479219406	27.055596210570332	28.635892658410096	21.89627633882551
39	22.051368236067866	26.92564719769321	28.933108017801164	22.08987654843777
40	21.896144007855167	27.182104618218535	29.05710213701284	21.864649236913458
41	21.939019242246395	26.64523787153574	29.04704498326675	22.368697902951112
42	21.656227958622754	27.140685024501078	29.178978960699027	22.024108056177145
43	22.060763734962237	26.766717702310892	29.061601390004512	22.110917172722353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	108.5
2	118.0
3	386.5
4	655.0
5	655.0
6	731.5
7	808.0
8	751.0
9	694.0
10	952.0
11	1210.0
12	1210.0
13	1909.5
14	2609.0
15	4971.5
16	7334.0
17	8657.0
18	9980.0
19	9980.0
20	9498.0
21	9016.0
22	10041.0
23	11066.0
24	13820.5
25	16575.0
26	16575.0
27	19773.0
28	22971.0
29	26419.5
30	29868.0
31	33043.5
32	36219.0
33	36219.0
34	39712.0
35	43205.0
36	45080.0
37	46955.0
38	48057.5
39	49160.0
40	49160.0
41	50248.0
42	51336.0
43	51437.0
44	51538.0
45	51287.0
46	51036.0
47	51036.0
48	51055.5
49	51075.0
50	50428.5
51	49782.0
52	49118.0
53	48454.0
54	48454.0
55	45319.0
56	42184.0
57	37913.5
58	33643.0
59	30821.5
60	28000.0
61	28000.0
62	24433.0
63	20866.0
64	17566.0
65	14266.0
66	11943.0
67	9620.0
68	9620.0
69	7871.0
70	6122.0
71	5208.5
72	4295.0
73	3229.5
74	2164.0
75	2164.0
76	1624.5
77	1085.0
78	833.0
79	581.0
80	477.5
81	374.0
82	374.0
83	311.5
84	249.0
85	230.5
86	212.0
87	177.0
88	142.0
89	142.0
90	112.5
91	83.0
92	55.5
93	28.0
94	16.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	755681.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.56596813003033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7575125291882	58.416637821886795
2	7.569877145340515	10.077924016099633
3	1.765027904754673	3.5247237376954135
4	0.7610990771715787	2.0265318765919518
5	0.43350278130911457	1.4428266162451013
6	0.30000283964311303	1.1981987677561237
7	0.207223681135429	0.9655831467973972
8	0.15228436686169028	0.8109565048973697
9	0.11615955416708021	0.6959045862615952
>10	0.8157867973964209	10.649169758593336
>50	0.08061492761988844	3.692261108283945
>100	0.03930414461302506	4.3848696088849
>500	8.021253996297818E-4	0.34292459495371197
>1k	6.015940497223364E-4	0.9807189564518963
>5k	2.0053134990744546E-4	0.7907688986009301
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5924	0.7839286683137461	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4074	0.5391163731786296	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2254	0.29827400715381225	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.13484525877982906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3233097034330622E-4	0.0
3	0.0	0.0	0.0	1.3233097034330622E-4	0.0
4	0.0	0.0	0.0	2.6466194068661245E-4	0.0
5	0.0	0.0	2.6466194068661245E-4	3.969929110299187E-4	0.0
6	0.0	0.0	0.0010586477627464498	3.969929110299187E-4	0.0
7	0.0	0.0	0.002646619406866125	3.969929110299187E-4	0.0
8	0.0	0.0	0.0030436123178960435	3.969929110299187E-4	0.0
9	0.0	0.0	0.003440605228925962	6.616548517165312E-4	0.0
10	2.6466194068661245E-4	0.0	0.0041022600806424935	6.616548517165312E-4	0.0
11	2.6466194068661245E-4	0.0	0.006351886576478699	9.263167924031437E-4	0.0
12	2.6466194068661245E-4	0.0	0.006351886576478699	0.001190978733089756	0.0
13	2.6466194068661245E-4	0.0	0.007013541428195231	0.0013233097034330624	0.0
14	2.6466194068661245E-4	0.0	0.0071458723985385365	0.0013233097034330624	0.0
15	2.6466194068661245E-4	0.0	0.00807218919094168	0.0014556406737763687	0.0
16	2.6466194068661245E-4	0.0	0.008469182101971598	0.0015879716441196748	0.0
17	3.969929110299187E-4	0.0	0.00913083695368813	0.0018526335848062874	0.0
18	3.969929110299187E-4	0.0	0.009395498894374743	0.0021172955254928996	0.0
19	3.969929110299187E-4	0.0	0.009527829864718049	0.0021172955254928996	0.0
20	3.969929110299187E-4	0.0	0.010057153746091275	0.002249626495836206	0.0
21	3.969929110299187E-4	0.0	0.010057153746091275	0.002646619406866125	0.0
22	3.969929110299187E-4	0.0	0.010057153746091275	0.0029112813475527374	0.0
23	3.969929110299187E-4	0.0	0.01018948471643458	0.0029112813475527374	0.0
24	3.969929110299187E-4	0.0	0.01018948471643458	0.003440605228925962	0.0
25	3.969929110299187E-4	0.0	0.01018948471643458	0.003705267169612575	0.0
26	3.969929110299187E-4	0.0	0.01018948471643458	0.004234591050985799	0.0
27	3.969929110299187E-4	0.0	0.01018948471643458	0.005822562695105475	0.0
28	3.969929110299187E-4	0.0	0.01018948471643458	0.01230678024192748	0.0
29	3.969929110299187E-4	0.0	0.01018948471643458	0.029245144445870678	0.0
30	3.969929110299187E-4	0.0	0.01018948471643458	0.056769986277278375	0.0
31	3.969929110299187E-4	0.0	0.01018948471643458	0.11499561322833311	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	40	5.937479E-5	27.75	7
GGTATCA	985	0.0	27.421318	1
TTAACGG	60	1.3363933E-6	24.666666	35
TTCGCCG	75	1.3722456E-8	24.666666	24
AACGGCC	55	1.9015404E-5	23.545454	37
GCCGCTC	90	3.8235157E-9	22.61111	27
GCCGGCA	100	5.329639E-10	22.199999	15
GTATCAA	2420	0.0	22.092976	1
GGACCGT	70	5.0981052E-6	21.142859	6
ACGGTAC	45	0.0038246135	20.555555	3
AACCACG	55	5.1408523E-4	20.181818	35
CGAGTCG	70	1.2184958E-4	18.5	21
TAACGGC	70	1.2184958E-4	18.5	36
CCGTCCG	60	9.2328247E-4	18.5	28
GACGTAT	50	0.0070327083	18.499998	28
GGACGTA	50	0.0070327083	18.499998	27
AAACACG	50	0.0070327083	18.499998	15
CCGTTTA	50	0.0070327083	18.499998	27
TTGCGGT	85	2.7211167E-5	17.411764	35
CACGTAG	85	2.7211167E-5	17.411764	26
>>END_MODULE