FastQCFastQC Report
Fri 10 Feb 2017
ERR1633409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555957
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35580.6399775522207652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.44607766427979145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14520.2611712776347811No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.10828175560340099No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5920.10648305534420827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTC250.00549393329.625
GGTATCA5950.025.8067231
GAGTCGC400.001929940123.12522
CGAGTCG502.699671E-422.221
GTATCAA15600.021.8205131
CCGTCCG450.00382336120.55555528
TCCGGCG555.138504E-420.18181816
TCGTCTT1101.7498678E-920.18181814
ATGCCCG1202.382876E-1020.04166829
CTCGTCT1403.6379788E-1219.82142813
TCTCGTC1153.0395313E-919.30434812
CCCTACA701.21776124E-418.52
TTGTCCG609.2286395E-418.513
GTCCGGC609.2286395E-418.515
CGGGAAT500.007030425618.56
CGCCGTC500.007030425618.526
CGTCCGC500.007030425618.529
TGTCCGA609.2286395E-418.512
GCGAGTC500.007030425618.520
GTTAGCC1156.3871994E-817.69565228
Produced by FastQC (version 0.11.5)