##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633409.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	555957
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53334880215556	33.0	33.0	33.0	27.0	33.0
2	31.728750604812962	33.0	33.0	33.0	27.0	33.0
3	31.445937365659574	33.0	33.0	33.0	27.0	33.0
4	31.7654746680049	33.0	33.0	33.0	27.0	33.0
5	31.86207746282536	33.0	33.0	33.0	33.0	33.0
6	34.92928230060958	37.0	37.0	37.0	27.0	37.0
7	35.08979471433942	37.0	37.0	37.0	33.0	37.0
8	35.23067071733965	37.0	37.0	37.0	33.0	37.0
9	35.35295895186138	37.0	37.0	37.0	33.0	37.0
10	35.346701273659654	37.0	37.0	37.0	33.0	37.0
11	35.364503010124885	37.0	37.0	37.0	33.0	37.0
12	35.37105027906834	37.0	37.0	37.0	33.0	37.0
13	35.37411346560975	37.0	37.0	37.0	33.0	37.0
14	35.35319098419482	37.0	37.0	37.0	33.0	37.0
15	35.36304786161519	37.0	37.0	37.0	33.0	37.0
16	35.34309667834023	37.0	37.0	37.0	33.0	37.0
17	35.347929785936685	37.0	37.0	37.0	33.0	37.0
18	35.32581836365043	37.0	37.0	37.0	33.0	37.0
19	35.31622409646789	37.0	37.0	37.0	33.0	37.0
20	35.31815949794678	37.0	37.0	37.0	33.0	37.0
21	35.30380407117816	37.0	37.0	37.0	33.0	37.0
22	35.20228183114881	37.0	37.0	37.0	33.0	37.0
23	35.23704531105823	37.0	37.0	37.0	33.0	37.0
24	35.23804358970208	37.0	37.0	37.0	33.0	37.0
25	35.252132808832336	37.0	37.0	37.0	33.0	37.0
26	35.14542671465599	37.0	37.0	37.0	33.0	37.0
27	35.1459771169353	37.0	37.0	37.0	33.0	37.0
28	35.17140174509899	37.0	37.0	37.0	33.0	37.0
29	35.15975875832124	37.0	37.0	37.0	33.0	37.0
30	35.168615558397505	37.0	37.0	37.0	33.0	37.0
31	35.166464312887506	37.0	37.0	37.0	33.0	37.0
32	35.143126177024484	37.0	37.0	37.0	33.0	37.0
33	35.1380034786863	37.0	37.0	37.0	33.0	37.0
34	35.1158632771959	37.0	37.0	37.0	33.0	37.0
35	35.040289087105656	37.0	37.0	37.0	27.0	37.0
36	35.064353897873396	37.0	37.0	37.0	27.0	37.0
37	35.05868619335668	37.0	37.0	37.0	33.0	37.0
38	35.038641477668236	37.0	37.0	37.0	27.0	37.0
39	34.940328118901284	37.0	37.0	37.0	27.0	37.0
40	34.7200215124551	37.0	37.0	37.0	27.0	37.0
41	34.88635452022368	37.0	37.0	37.0	27.0	37.0
42	34.925823400011154	37.0	37.0	37.0	27.0	37.0
43	34.55885617053117	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	21.0
16	25.0
17	27.0
18	22.0
19	17.0
20	58.0
21	153.0
22	389.0
23	929.0
24	1796.0
25	3146.0
26	4869.0
27	6968.0
28	9745.0
29	12845.0
30	16439.0
31	20456.0
32	25882.0
33	34283.0
34	48382.0
35	93723.0
36	275778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93733867907051	21.23185785951072	14.115660024066608	23.715143437352168
2	15.684666260160407	22.663443395802194	39.36383569232873	22.288054651708674
3	17.58157555350504	28.0043240754231	31.057617765402718	23.356482605669143
4	12.143205319835888	18.807749520196708	40.57220252645438	28.476842633513023
5	13.335743591680652	37.393539428409035	35.70509949510484	13.565617484805479
6	27.63666974244411	41.37388323197657	17.858755263446632	13.130691762132681
7	25.045282279025177	33.46949494295422	23.918576436666864	17.566646341353735
8	24.02559910208883	36.151536899436465	21.111884552222566	18.71097944625214
9	25.812248069544946	15.682687689875296	20.893882080808407	37.61118215977135
10	14.504898760155912	28.901156024656583	34.1787224551539	22.415222760033597
11	32.75972782067678	24.490742989116065	24.814329165744834	17.935200024462322
12	22.361981232361497	26.453304841921227	31.32184683347813	19.86286709223915
13	27.665269076565274	23.134163253632924	26.79631698134928	22.404250688452525
14	21.469646033775994	21.95385614355067	29.15405328109908	27.422444541574258
15	22.98990749284567	29.515232293144972	25.598742348778774	21.896117865230586
16	21.66840241241679	29.55264525853618	26.124682304566722	22.65427002448031
17	21.480797975382988	28.304347278656444	27.39654325784188	22.818311488118688
18	22.3209708664519	27.64116649309209	29.02058972186698	21.01727291858903
19	23.967680953742825	26.827254625807388	28.860865138850667	20.344199281599114
20	23.70830837636724	26.70404365805269	28.752403513221346	20.835244452358726
21	22.433029892599606	27.46939061833919	28.636926956581178	21.46065253248003
22	21.79089390006781	27.586306135186717	28.80006907008995	21.822730894655525
23	21.933350960595874	27.202103759823153	29.224562331259428	21.639982948321542
24	21.918062008392734	27.50050813282322	28.47036731257993	22.111062546204113
25	22.182650816519985	27.381973785742424	28.982817016423933	21.452558381313665
26	22.350109810650824	27.55213083026205	28.616601643652295	21.481157715434826
27	22.07742685135721	27.734878776596034	28.357049196250788	21.830645175795972
28	21.801686101622966	27.602134697467612	28.779204147083316	21.816975053826106
29	22.741686857077077	27.418127660952194	28.346976474799312	21.49320900717142
30	22.196860548567603	27.366325093487447	29.004761159586085	21.432053198358865
31	22.224200792507336	27.27423164021678	28.722724958944667	21.77884260833122
32	21.388344782060482	27.142566781243872	29.071133199150296	22.39795523754535
33	21.437629169162363	27.44169063434762	29.58700043348676	21.533679763003256
34	21.96662691539094	27.302111494234264	28.697902895367804	22.03335869500699
35	22.016450912570576	27.753405389265716	28.55814388522853	21.671999812935173
36	22.47224155825001	27.368843273850317	28.41550695467455	21.743408213225123
37	22.365039022802126	27.061085659502442	28.48205886426468	22.091816453430752
38	22.323129306762933	27.194189478682706	28.580088028390684	21.902593186163678
39	21.915723698055785	27.19778687920109	28.779923627186992	22.106565795556133
40	21.791613380171487	27.754124869369395	28.83352489491094	21.62073685554818
41	21.871655541705564	26.74415467383269	29.01735206140043	22.366837723061316
42	21.644299828943605	27.529647077022148	28.89719888408636	21.928854209947893
43	22.124912538199897	26.91808898889662	28.6549139591731	22.302084513730378
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	53.0
2	52.0
3	199.0
4	346.0
5	346.0
6	423.0
7	500.0
8	469.0
9	438.0
10	597.0
11	756.0
12	756.0
13	1228.5
14	1701.0
15	3217.5
16	4734.0
17	5589.0
18	6444.0
19	6444.0
20	6359.5
21	6275.0
22	7200.5
23	8126.0
24	10130.0
25	12134.0
26	12134.0
27	14613.0
28	17092.0
29	19434.0
30	21776.0
31	24260.5
32	26745.0
33	26745.0
34	29594.5
35	32444.0
36	34068.5
37	35693.0
38	36744.0
39	37795.0
40	37795.0
41	38728.5
42	39662.0
43	40129.0
44	40596.0
45	39693.5
46	38791.0
47	38791.0
48	38111.5
49	37432.0
50	37377.5
51	37323.0
52	36802.0
53	36281.0
54	36281.0
55	34046.5
56	31812.0
57	27871.0
58	23930.0
59	21188.0
60	18446.0
61	18446.0
62	16147.5
63	13849.0
64	11404.5
65	8960.0
66	7527.0
67	6094.0
68	6094.0
69	4982.5
70	3871.0
71	3233.0
72	2595.0
73	2012.5
74	1430.0
75	1430.0
76	1063.0
77	696.0
78	547.5
79	399.0
80	324.5
81	250.0
82	250.0
83	212.5
84	175.0
85	152.5
86	130.0
87	106.5
88	83.0
89	83.0
90	59.5
91	36.0
92	23.5
93	11.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	555957.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.65164280590285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.27502660230518	56.188504619715005
2	6.772988369827426	8.622236728895793
3	1.7359460442585313	3.314874526183921
4	0.7803619906441936	1.9868529075115007
5	0.4469689384070828	1.4225153606410614
6	0.31054269129061435	1.1859931477208365
7	0.23361149645241444	1.040882886927907
8	0.16472445198994665	0.8387985583569741
9	0.1410197755187861	0.807852634190134
>10	0.9744023956999328	12.243035418432394
>50	0.10646511032551788	4.613047133188598
>100	0.05651498714046865	6.1576935483864625
>500	5.708584559643142E-4	0.21692638883466542
>1k	8.562876839464715E-4	1.3607861410147772
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3558	0.6399775522207652	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2480	0.44607766427979145	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1452	0.2611712776347811	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.10828175560340099	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	592	0.10648305534420827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.597400518385415E-4	0.0	0.0
2	0.0	0.0	3.597400518385415E-4	0.0	0.0
3	0.0	0.0	5.396100777578122E-4	0.0	0.0
4	0.0	0.0	5.396100777578122E-4	1.7987002591927074E-4	0.0
5	0.0	0.0	5.396100777578122E-4	1.7987002591927074E-4	0.0
6	0.0	0.0	0.0012590901814348951	1.7987002591927074E-4	0.0
7	1.7987002591927074E-4	0.0	0.003237660466546873	1.7987002591927074E-4	0.0
8	1.7987002591927074E-4	0.0	0.004316880622062497	1.7987002591927074E-4	0.0
9	1.7987002591927074E-4	0.0	0.005755840829416664	8.993501295963537E-4	0.0
10	3.597400518385415E-4	0.0	0.006475320933093746	8.993501295963537E-4	0.0
11	3.597400518385415E-4	0.0	0.010252591477398432	0.0010792201555156243	0.0
12	3.597400518385415E-4	0.0	0.010612331529236973	0.0010792201555156243	0.0
13	3.597400518385415E-4	0.0	0.011151941606994786	0.0010792201555156243	0.0
14	3.597400518385415E-4	0.0	0.011511681658833327	0.0010792201555156243	0.0
15	3.597400518385415E-4	0.0	0.01205129173659114	0.0012590901814348951	0.0
16	3.597400518385415E-4	0.0	0.012770771840268222	0.0012590901814348951	0.0
17	3.597400518385415E-4	0.0	0.012950641866187492	0.0017987002591927073	0.0
18	3.597400518385415E-4	0.0	0.012950641866187492	0.001978570285111978	0.0
19	3.597400518385415E-4	0.0	0.013310381918026035	0.001978570285111978	0.0
20	3.597400518385415E-4	0.0	0.013490251943945306	0.001978570285111978	0.0
21	3.597400518385415E-4	0.0	0.014029862021703117	0.0021584403110312487	1.7987002591927074E-4
22	3.597400518385415E-4	0.0	0.014389602073541658	0.0025181803628697903	1.7987002591927074E-4
23	3.597400518385415E-4	0.0	0.014389602073541658	0.003237660466546873	1.7987002591927074E-4
24	3.597400518385415E-4	0.0	0.014389602073541658	0.003957140570223956	1.7987002591927074E-4
25	3.597400518385415E-4	0.0	0.014569472099460929	0.003957140570223956	1.7987002591927074E-4
26	3.597400518385415E-4	0.0	0.014569472099460929	0.004676620673901039	1.7987002591927074E-4
27	3.597400518385415E-4	0.0	0.014569472099460929	0.005216230751658851	1.7987002591927074E-4
28	3.597400518385415E-4	0.0	0.014569472099460929	0.011151941606994786	1.7987002591927074E-4
29	3.597400518385415E-4	0.0	0.014569472099460929	0.02626102378421353	1.7987002591927074E-4
30	3.597400518385415E-4	0.0	0.014569472099460929	0.04946425712779945	1.7987002591927074E-4
31	3.597400518385415E-4	0.0	0.014569472099460929	0.09515124371129421	1.7987002591927074E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTC	25	0.005493933	29.6	25
GGTATCA	595	0.0	25.806723	1
GAGTCGC	40	0.0019299401	23.125	22
CGAGTCG	50	2.699671E-4	22.2	21
GTATCAA	1560	0.0	21.820513	1
CCGTCCG	45	0.003823361	20.555555	28
TCCGGCG	55	5.138504E-4	20.181818	16
TCGTCTT	110	1.7498678E-9	20.181818	14
ATGCCCG	120	2.382876E-10	20.041668	29
CTCGTCT	140	3.6379788E-12	19.821428	13
TCTCGTC	115	3.0395313E-9	19.304348	12
CCCTACA	70	1.21776124E-4	18.5	2
TTGTCCG	60	9.2286395E-4	18.5	13
GTCCGGC	60	9.2286395E-4	18.5	15
CGGGAAT	50	0.0070304256	18.5	6
CGCCGTC	50	0.0070304256	18.5	26
CGTCCGC	50	0.0070304256	18.5	29
TGTCCGA	60	9.2286395E-4	18.5	12
GCGAGTC	50	0.0070304256	18.5	20
GTTAGCC	115	6.3871994E-8	17.695652	28
>>END_MODULE