FastQCFastQC Report
Fri 10 Feb 2017
ERR1633408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681229
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123241.8090832891729507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91461.3425734958435416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49640.7286830126139668No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25570.3753510199947448No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22720.3335148679812515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21680.3182483423342224No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG18730.274944255162361No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16700.2451451714474868No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15790.23178696150633632No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14340.21050190170999764No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13350.19596934364215263No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12880.18907004839782218No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12850.1886296678503117No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.18334510128018625No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12330.18099640502679715No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12220.17938167635259214No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11220.1647023247689103No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11090.16279400906303165No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10670.1566286813978853No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10640.15618830085037483No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10490.15398639811282255No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10270.15075694076441257No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10110.14840824451102347No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9930.14576596122596072No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9900.14532558067845028No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9890.14517878716261345No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9880.14503199364677666No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9690.1422429168458771No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9660.14180253629836662No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9630.14136215575085617No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9570.14048139465583528No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9340.13710514379158845No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9160.1344628605065257No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9110.1337288929273416No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8920.13093981612644207No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA8910.13079302261060524No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8880.1303526420630948No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8620.12653601065133752No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8580.12594883658799022No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8580.12594883658799022No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8380.12301296627125387No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8330.12227899869206976No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8300.12183861814455933No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8260.12125144408121204No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8240.1209578570495384No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8230.1208110635337016No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8220.12066427001786477No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8200.12037068298619114No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8070.1184623672803125No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8030.11787519321696523No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7980.11714122563778112No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7850.1152329099319025No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7770.11405856180520793No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7660.11244383313100292No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7620.11185665906765566No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7460.10950796281426656No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7430.10906758226675611No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7380.10833361468757202No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7380.10833361468757202No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7290.10701247304504065No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7280.10686567952920385No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7250.10642529898169338No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7190.10554453788667248No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7160.10510415733916202No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7150.10495736382332518No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7140.10481057030748839No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.10334263514912019No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7020.10304904811744656No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6950.10202149350658882No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6950.10202149350658882No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6940.10187469999075201No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6940.10187469999075201No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6900.10128752592740474No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6890.10114073241156792No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6880.1009939388957311No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6860.10070035186405746No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6840.10040676483238381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTT200.001841409437.019
GCTCTAG303.597953E-430.8333341
AAGTATT250.005494790629.632
AAGAGTC250.005494790629.620
AGAGTCA250.005494790629.621
ACGCTGA250.005494790629.634
GAATTAG250.005494790629.618
CGTCAAA250.005494790629.627
GAGATCA250.005494790629.69
CTAGTGC405.93663E-527.7500029
GGTATAC405.93663E-527.7500023
TAGTGCA405.93663E-527.75000210
GCTCTCT2750.026.90909230
ACAACGC358.8656717E-426.428573
CGCTCTC2800.026.4285729
GCCGTCA358.8656717E-426.4285720
CCGCTCT2850.025.96491228
GCCGCTC2950.025.71186427
GTATACT451.3224389E-424.6666684
GGTTATG451.3224389E-424.6666681
Produced by FastQC (version 0.11.5)