FastQCFastQC Report
Fri 10 Feb 2017
ERR1633407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076282
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61630.5726194436030706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.40983682715124853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23590.21918047500562118No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC14330.13314354416407595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.10322573451939177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG303.599328E-430.8333329
GGTATCA9650.025.497411
CGTATGG451.3231357E-424.66666610
CCGTATG451.3231357E-424.6666669
GTATCAA27600.024.1974621
ACCGTAT753.7403152E-722.28
CGCCTAT450.00382565220.55555536
CGGTCGT450.00382565220.55555510
TCTATCG450.00382565220.55555531
TTAACGG1700.019.58823435
CGAGTCG1052.259003E-819.3809521
CATAATA2050.018.9512182
GTACCGG500.007034598418.56
GCCGCTC1750.017.9714327
CCGTTAA1552.0008883E-1117.90322516
CGCCGTC1054.796566E-717.61904726
GCCGTCC1054.796566E-717.61904727
CCGCTCT1950.017.07692328
CGCTCTC2100.016.73809429
CCGTCCG1005.877091E-616.65000228
Produced by FastQC (version 0.11.5)