##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1076282
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.881303413046023	33.0	33.0	33.0	33.0	33.0
2	31.986621535991496	33.0	33.0	33.0	33.0	33.0
3	31.70513118309142	33.0	33.0	33.0	27.0	33.0
4	31.98460161927822	33.0	33.0	33.0	33.0	33.0
5	32.07754844919826	33.0	33.0	33.0	33.0	33.0
6	35.24345849879492	37.0	37.0	37.0	33.0	37.0
7	35.40178503403383	37.0	37.0	37.0	33.0	37.0
8	35.53959092505496	37.0	37.0	37.0	33.0	37.0
9	35.648880126212276	37.0	37.0	37.0	33.0	37.0
10	35.65166099590999	37.0	37.0	37.0	33.0	37.0
11	35.68238435651623	37.0	37.0	37.0	33.0	37.0
12	35.66468267610162	37.0	37.0	37.0	33.0	37.0
13	35.669345022958666	37.0	37.0	37.0	33.0	37.0
14	35.6564218299665	37.0	37.0	37.0	33.0	37.0
15	35.673004844455264	37.0	37.0	37.0	33.0	37.0
16	35.656767464289096	37.0	37.0	37.0	33.0	37.0
17	35.65071793451902	37.0	37.0	37.0	33.0	37.0
18	35.63389892240138	37.0	37.0	37.0	33.0	37.0
19	35.62365718278295	37.0	37.0	37.0	33.0	37.0
20	35.62465041689817	37.0	37.0	37.0	33.0	37.0
21	35.623426759901214	37.0	37.0	37.0	33.0	37.0
22	35.5308023361907	37.0	37.0	37.0	33.0	37.0
23	35.57678656708929	37.0	37.0	37.0	33.0	37.0
24	35.577149854777836	37.0	37.0	37.0	33.0	37.0
25	35.600928938698225	37.0	37.0	37.0	33.0	37.0
26	35.4830982957998	37.0	37.0	37.0	33.0	37.0
27	35.489289981621916	37.0	37.0	37.0	33.0	37.0
28	35.51610172798579	37.0	37.0	37.0	33.0	37.0
29	35.51907399733527	37.0	37.0	37.0	33.0	37.0
30	35.52556671950288	37.0	37.0	37.0	33.0	37.0
31	35.52194685036078	37.0	37.0	37.0	33.0	37.0
32	35.50068197739998	37.0	37.0	37.0	33.0	37.0
33	35.49476717068575	37.0	37.0	37.0	33.0	37.0
34	35.480363882328234	37.0	37.0	37.0	33.0	37.0
35	35.41116733346836	37.0	37.0	37.0	33.0	37.0
36	35.44816042635666	37.0	37.0	37.0	33.0	37.0
37	35.441388966832115	37.0	37.0	37.0	33.0	37.0
38	35.437535887434706	37.0	37.0	37.0	33.0	37.0
39	35.333140385140695	37.0	37.0	37.0	33.0	37.0
40	35.11396455575769	37.0	37.0	37.0	33.0	37.0
41	35.28448120474002	37.0	37.0	37.0	33.0	37.0
42	35.32755820500575	37.0	37.0	37.0	33.0	37.0
43	35.00772102478719	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	31.0
16	60.0
17	35.0
18	30.0
19	51.0
20	63.0
21	143.0
22	453.0
23	1051.0
24	2169.0
25	3997.0
26	6543.0
27	10172.0
28	14331.0
29	19588.0
30	25748.0
31	33300.0
32	42779.0
33	56389.0
34	81934.0
35	166675.0
36	610735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.508545158239194	20.221187383975575	13.870063793689758	24.400203664095468
2	15.953997186610943	21.674152313241322	39.37090836788128	23.00094213226645
3	17.825253976188396	27.386595706329754	30.16848744102382	24.61966287645803
4	12.479257295021192	17.86966612839386	39.77544918525071	29.87562739133424
5	13.67281065742993	37.22955507943085	35.14673663593742	13.950897627201792
6	28.941392683330204	40.10956236376712	17.53285848876038	13.416186464142296
7	26.18235741190506	32.45190386906034	23.615929654124105	17.749809064910497
8	24.51894577815108	35.679961199759916	20.75441194779807	19.046681074290937
9	26.17380946629229	15.421887572216203	20.363900910727857	38.04040205076365
10	15.297199061212583	28.064763695760032	33.65604925103272	22.98198799199466
11	33.781481061654844	24.05958661391717	24.11635612228022	18.042576202147764
12	23.120985020654437	25.81303041396214	30.123424901652168	20.942559663731252
13	28.165387881614667	22.27120773180263	26.22741995127671	23.33598443530599
14	22.221220832458407	21.064182063808556	28.111405746821	28.603191356912035
15	23.853692619592263	28.58470177890181	24.81050505350828	22.751100547997645
16	22.67416903748274	28.10109246461429	25.45578203481987	23.768956463083097
17	22.229118390904986	27.309664195814854	26.6067814940694	23.854435919210765
18	23.274662216779618	26.63865046521265	27.833690426858386	22.252996891149344
19	24.95479809194988	25.886338338836843	27.659758316129047	21.499105253084227
20	24.11905058339729	26.218314530950064	27.72860644329274	21.934028442359903
21	23.43335668532968	26.67804534499323	27.642476599998883	22.246121369678207
22	22.89567232379618	26.496866062983493	27.794295547077812	22.813166066142518
23	22.847264936141272	26.514519428922906	28.07303290401586	22.565182730919965
24	22.99926970812482	26.78275767874962	27.23171064832451	22.986261964801045
25	23.096734870600827	26.54248607706902	28.194748216545477	22.16603083578467
26	23.22114464424751	26.5943312254595	27.911922711705667	22.27260141858732
27	23.128232191934828	26.653609370034992	27.36160225665764	22.85655618137254
28	22.674261949935055	26.472430088025256	28.118374180744453	22.734933781295236
29	23.601714048920265	26.35656825999134	27.471610600195863	22.570107090892535
30	23.39972237759249	26.49147714074936	27.716156174682844	22.39264430697531
31	23.172458519235665	26.278893449857936	27.721545096916977	22.82710293398942
32	22.217690159270525	26.37998219797414	28.126643389000282	23.27568425375506
33	22.47431435255816	26.530407458268375	28.18369163472027	22.8115865544532
34	22.87012139941019	26.35907689620378	27.950667204320055	22.820134500065965
35	23.059569889675753	26.685013778916677	27.67806206923464	22.577354262172925
36	23.54828938884047	26.5511269351341	27.18367491047885	22.716908765546577
37	23.284975498986324	26.108120362507226	27.54984288504314	23.057061253463313
38	23.331710462499604	26.496122763364994	27.25354507461799	22.918621699517413
39	22.54000345634323	26.533287744290064	27.809626101709405	23.117082697657303
40	22.9683298615047	26.568873213525823	28.062812534261468	22.39998439070801
41	22.732703882439733	26.017344896597734	27.92232890636469	23.32762231459785
42	22.199572231069553	27.070321718657375	27.723496258415548	23.006609791857525
43	23.146443032588113	25.88327222791053	27.751834556370913	23.218450183130443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	73.5
2	105.0
3	366.5
4	628.0
5	628.0
6	724.0
7	820.0
8	787.5
9	755.0
10	1000.5
11	1246.0
12	1246.0
13	2085.5
14	2925.0
15	5377.0
16	7829.0
17	8942.0
18	10055.0
19	10055.0
20	9156.0
21	8257.0
22	8926.0
23	9595.0
24	12289.0
25	14983.0
26	14983.0
27	18917.5
28	22852.0
29	27536.5
30	32221.0
31	36919.5
32	41618.0
33	41618.0
34	48945.5
35	56273.0
36	60995.0
37	65717.0
38	69646.5
39	73576.0
40	73576.0
41	77295.5
42	81015.0
43	82057.0
44	83099.0
45	82651.0
46	82203.0
47	82203.0
48	80416.5
49	78630.0
50	77481.5
51	76333.0
52	73370.0
53	70407.0
54	70407.0
55	67987.0
56	65567.0
57	58116.5
58	50666.0
59	46248.0
60	41830.0
61	41830.0
62	37647.5
63	33465.0
64	27000.5
65	20536.0
66	17261.0
67	13986.0
68	13986.0
69	11718.0
70	9450.0
71	7898.0
72	6346.0
73	6112.0
74	5878.0
75	5878.0
76	4952.5
77	4027.0
78	3002.5
79	1978.0
80	1321.5
81	665.0
82	665.0
83	496.5
84	328.0
85	265.5
86	203.0
87	161.0
88	119.0
89	119.0
90	80.0
91	41.0
92	26.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1076282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.77914229681529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14443146323632	47.49283358369294
2	8.973246748239442	10.010400144689658
3	2.206937341327078	3.6930321600611493
4	0.9117067355490888	2.0341687894063027
5	0.5359031113765741	1.4946107953389989
6	0.3137934546076948	1.0501877853823114
7	0.24484055966927445	0.9559897492477046
8	0.1838100238920987	0.8202212382606713
9	0.1388708848230072	0.6971488960884448
>10	1.0875814777281512	12.297283799452833
>50	0.15358197951285682	6.003438250997321
>100	0.1002339020399238	10.43373084253373
>500	0.004218598331987587	1.560198075728338
>1k	6.74975733118014E-4	0.8766701784105778
>5k	1.687439332795035E-4	0.5800857107090822
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6163	0.5726194436030706	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4411	0.40983682715124853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2359	0.21918047500562118	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1433	0.13314354416407595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1111	0.10322573451939177	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.7873735693805153E-4	0.0	0.0
2	1.858249046253677E-4	0.0	2.7873735693805153E-4	0.0	0.0
3	1.858249046253677E-4	0.0	5.574747138761031E-4	0.0	0.0
4	1.858249046253677E-4	0.0	8.362120708141547E-4	0.0	0.0
5	1.858249046253677E-4	0.0	0.0011149494277522061	0.0	0.0
6	2.7873735693805153E-4	0.0	0.0021369864031917287	0.0	0.0
7	2.7873735693805153E-4	0.0	0.004738535067946877	0.0	0.0
8	3.716498092507354E-4	0.0	0.005760572043386399	0.0	0.0
9	3.716498092507354E-4	0.0	0.007432996185014708	6.50387166188787E-4	0.0
10	8.362120708141547E-4	0.0	0.008919595422017649	6.50387166188787E-4	0.0
11	8.362120708141547E-4	0.0	0.013658130489964527	8.362120708141547E-4	0.0
12	8.362120708141547E-4	0.0	0.0149589048223421	9.291245231268385E-4	0.0
13	8.362120708141547E-4	0.0	0.016817153868595777	0.0010220369754395224	0.0
14	8.362120708141547E-4	0.0	0.017932103296347982	0.0010220369754395224	0.0
15	8.362120708141547E-4	0.0	0.020162002151852397	0.0011149494277522061	0.0
16	8.362120708141547E-4	0.0	0.021369864031917287	0.0011149494277522061	0.0
17	8.362120708141547E-4	0.0	0.02202025119810607	0.00120786188006489	0.0
18	9.291245231268385E-4	0.0	0.022577725911982177	0.001300774332377574	0.0
19	9.291245231268385E-4	0.0	0.02313520062585828	0.0013936867846902577	0.0
20	9.291245231268385E-4	0.0	0.023692675339734383	0.0016724241416283094	0.0
21	0.0010220369754395224	0.0	0.02564383683830074	0.001858249046253677	0.0
22	0.0010220369754395224	0.0	0.025922574195238794	0.002322811307817096	0.0
23	0.0010220369754395224	0.0	0.026294224004489528	0.002508636212442464	0.0
24	0.0010220369754395224	0.0	0.02657296136142758	0.0027873735693805155	0.0
25	0.0010220369754395224	0.0	0.02657296136142758	0.0028802860216931995	0.0
26	0.0010220369754395224	0.0	0.02657296136142758	0.0033448482832566188	0.0
27	0.0010220369754395224	0.0	0.026665873813740266	0.004738535067946877	0.0
28	0.0011149494277522061	0.0	0.026665873813740266	0.01570220444084357	0.0
29	0.0011149494277522061	0.0	0.026665873813740266	0.03642168130657207	0.0
30	0.0011149494277522061	0.0	0.026665873813740266	0.06104348116943329	0.0
31	0.0011149494277522061	0.0	0.026665873813740266	0.12301608686199342	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTCG	30	3.599328E-4	30.833332	9
GGTATCA	965	0.0	25.49741	1
CGTATGG	45	1.3231357E-4	24.666666	10
CCGTATG	45	1.3231357E-4	24.666666	9
GTATCAA	2760	0.0	24.197462	1
ACCGTAT	75	3.7403152E-7	22.2	8
CGCCTAT	45	0.003825652	20.555555	36
CGGTCGT	45	0.003825652	20.555555	10
TCTATCG	45	0.003825652	20.555555	31
TTAACGG	170	0.0	19.588234	35
CGAGTCG	105	2.259003E-8	19.38095	21
CATAATA	205	0.0	18.951218	2
GTACCGG	50	0.0070345984	18.5	6
GCCGCTC	175	0.0	17.97143	27
CCGTTAA	155	2.0008883E-11	17.903225	16
CGCCGTC	105	4.796566E-7	17.619047	26
GCCGTCC	105	4.796566E-7	17.619047	27
CCGCTCT	195	0.0	17.076923	28
CGCTCTC	210	0.0	16.738094	29
CCGTCCG	100	5.877091E-6	16.650002	28
>>END_MODULE