FastQCFastQC Report
Fri 10 Feb 2017
ERR1633406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954809
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255182.6725764000967733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188141.9704464453100046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105631.1062945573407876No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG50230.5260738011476641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44850.469727453344072No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA44370.4647002698969113No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA41410.4336993053060874No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG38810.40646872830063396No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT33430.35012238049704186No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA32530.34069641153361563No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG31730.3323177724550146No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30510.31954034786014796No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA28600.2995363470599879No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.2827790689027858No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA24500.25659582178215745No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT23010.24099060649826304No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA22960.2404669415558504No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT22540.2360681560395849No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT22090.23135517155787177No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC21940.2297841767306341No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21770.22800371592643134No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC21510.225280658225886No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC21450.22465226029499097No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19250.201611002828838No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG19070.19972580903615278No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18510.19386076168113203No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC18360.19228976685389434No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC18150.19009037409576157No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC17980.18830991329155883No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC17810.1865294524873561No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17800.1864247194988736No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA17630.18464425869467088No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA17320.18139753605171297No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17210.1802454731784053No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA16790.17584668766213976No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA16510.1729141639846294No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA16310.17081950421497913No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG16230.16998164030711901No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG15660.16401185996361575No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC15320.16045093835521032No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC15290.16013673938976278No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15240.1596130744473502No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC15190.15908940950493763No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT15090.15804207962011252No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15090.15804207962011252No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT15060.15772788065466498No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT14790.15490008996563712No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG14780.15479535697715457No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14390.15071077042633657No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG14210.14882557663365134No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT13900.14557885399069342No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT13820.1447409900828333No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG13820.1447409900828333No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA13440.14076113652049782No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13310.13939960767022516No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA13310.13939960767022516No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG13230.13856174376236505No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.13772387985450493No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA12870.13479135617699456No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG12800.13405822525761696No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT12640.13238249744189676No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG12450.130392570660729No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12420.13007837169528147No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12310.12892630882197384No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC12220.12798371192563118No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC12210.12787897893714867No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12180.12756477997170115No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT12070.1264127170983935No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG12060.12630798410991098No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12000.1256795861790159No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC11980.1254701202020509No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA11920.12484172227115581No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG11750.12306126146695308No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11650.12201393158212794No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG11570.12117606767426785No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA11570.12117606767426785No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG11480.1202334707779252No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA11480.1202334707779252No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.11761514606586239No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11170.1169867481349673No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA11120.11646308319255474No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA11080.11604415123862469No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC11050.11572995227317716No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA10980.11499682135379956No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT10840.11353055951504437No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG10810.11321636054959683No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG10670.11175009871084166No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC10580.11080750181449903No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA10490.10986490491815641No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC10420.10913177399877881No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.10756077917154112No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9930.10399985756313566No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG9880.1034761926207231No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC9860.10326672664375806No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9830.10295252767831052No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG9640.10096260089714278No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9600.10054366894321272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC251.2334692E-437.01
CTGGTTG352.3878874E-531.71428733
TGTCAAC303.599026E-430.8333342
CTATAAG250.005495883529.61
GTTTCAA405.9390943E-527.751
GAATCAA659.38835E-825.6153852
TAAATGA951.0913936E-1125.315794
GTCTCAA451.3229832E-424.6666661
AAGCCAA1002.0008883E-1124.05000122
GCTTCTA1002.0008883E-1124.05000116
AAATGAT1002.0008883E-1124.0500015
AGCAACG551.9022222E-523.5454542
TAGCAAC551.9022222E-523.5454541
TGATCTA551.9022222E-523.54545420
TTAGATC400.001930949423.1253
TCTCAAC400.001930949423.1252
TGGGCAG400.001930949423.12531
CCAGTGC400.001930949423.1258
GGTATCA49000.023.0683671
CTTTAAA1054.0017767E-1122.904761
Produced by FastQC (version 0.11.5)