FastQCFastQC Report
Fri 10 Feb 2017
ERR1633405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583351
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104141.7852030767068199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83331.4284710234490041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46470.7966044456939304No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA36080.6184955541346462No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA26800.45941465772750884No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG24560.421015820663717No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23520.4031877891698137No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT20480.3510750817260963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.3221045305485034No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16230.27822014533274136No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC16000.2742774075985127No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15510.26587766199080826No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC15220.26090638397808524No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14180.24307835248418191No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13950.2391356147499533No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC13670.23433576011697932No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13380.22936448210425625No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA13110.22473605085103135No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12830.21993619621805738No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12730.21822196242056668No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12660.2170219987623232No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12330.21136502723060388No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12030.20622232583813174No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC12000.20570805569888453No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11770.2017653179646559No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11650.1997082374076671No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11530.19765115685067822No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11210.19216560869870797No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT11190.19182276193920983No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11080.18993710476197007No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11020.18890856448347565No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10270.17605181100229536No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10070.17262334340731397No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG9950.1705662628503251No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9610.1647378679388567No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9440.16182367048312252No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9410.1613094003438753No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9380.16079513020462807No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9330.15993801330588273No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9290.15925231978688645No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9220.15805235612864296No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT9210.1578809327488939No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8930.15308107811591992No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8920.15290965473617085No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8860.15188111445767644No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG8720.14948118714118944No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT8720.14948118714118944No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8520.14605271954620802No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA8460.1450241792677136No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG8460.1450241792677136No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8390.14382421560947012No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8340.14296709871072477No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8290.1421099818119794No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8190.1403957480144887No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA8160.1398814778752415No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7840.13439592972327125No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7800.13371023620427494No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7740.13268169592578052No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7630.13079603874854076No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7620.1306246153687917No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT7480.1282246880523047No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC7410.1270247243940612No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7380.126510454254814No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7290.12496764383707236No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7240.12411052693832703No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7160.12273913990033444No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6990.11982494244460024No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6930.11879640216610583No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6910.1184535554066077No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC6850.11742501512811325No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6800.11656789822936792No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6760.11588220471037163No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6650.11399654753313185No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6570.1126251604951393No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6570.1126251604951393No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6560.11245373711539022No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6310.10816815262166346No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6300.10799672924191439No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA6260.10731103572291811No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6210.10645391882417275No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA6110.10473968502668204No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT6060.10388256812793671No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG6010.10302545122919135No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6000.10285402784944227No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5900.10113979405195156No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5840.10011125377345714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAGTC200.001841193437.018
AAAATAC200.001841193437.037
CTAGTGG303.597324E-430.8333349
TTATATG250.00549415229.62
GTTATAT250.00549415229.61
CGCTCTC3450.025.7391329
GGTATCA23050.025.0412161
CCGCTCT3550.025.01408428
CAGCTTC3600.024.15277720
GCCGCTC3700.024.027
CCGGCAG3750.023.18666816
GGCAGCT3600.023.12518
GCTCTCT3850.023.06493430
GCAGCTT3700.023.019
TTCGCCG3950.022.94936824
GCCGGCA4000.022.215
GGCGAGT3350.022.08955219
GTATCAA53000.021.083021
GAGTCGC3600.021.06944522
CTCTCTT4350.020.8390831
Produced by FastQC (version 0.11.5)