FastQCFastQC Report
Fri 10 Feb 2017
ERR1633404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049030
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27210.2593824771455535No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20520.19560927714174048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.17082447594444392No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16330.15566761675071256No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16000.15252185352182493No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15790.150520004194351No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15520.1479461979161702No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14890.14194064993374833No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14690.14003412676472551No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12410.11829976263786546No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA11970.11410541166601527No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11660.11115030075402992No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG11620.11076899612022534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11120.10600268819766832No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA10790.10285692496878068No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA10700.10199898954272042No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10580.10085507564130673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8450.021.017751
GTATCAA20950.020.3985671
GTCGGTA759.264508E-619.73333420
TCGGTAA801.6164513E-518.521
GATAGCG609.2360814E-418.525
ACGGTAC609.2360814E-418.53
CGAGTCG500.00703448418.49999821
GTACTAT1950.018.0256421
ATATAGC3100.017.9032276
TTAACGG3900.017.55128335
CGCCGAT852.722749E-517.41176437
GCGTCGG852.722749E-517.41176418
GTACATG4900.017.3673461
CGTCCGC752.0670496E-417.26666629
AGTCGCC650.001579809617.07692323
GTCGCCC4350.017.01149437
CATGCTA4850.016.7835064
AACGGCC4150.016.49397737
TATCGCC904.445362E-516.44444533
CATAATA2600.016.3653852
Produced by FastQC (version 0.11.5)