##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633404.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1049030
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67922271050399	33.0	33.0	33.0	27.0	33.0
2	31.826638894979173	33.0	33.0	33.0	33.0	33.0
3	31.55372772942623	33.0	33.0	33.0	27.0	33.0
4	31.853521824923977	33.0	33.0	33.0	27.0	33.0
5	31.95877143646988	33.0	33.0	33.0	33.0	33.0
6	35.088202434630084	37.0	37.0	37.0	33.0	37.0
7	35.25015109196114	37.0	37.0	37.0	33.0	37.0
8	35.368243043573585	37.0	37.0	37.0	33.0	37.0
9	35.49430426203254	37.0	37.0	37.0	33.0	37.0
10	35.48988589458833	37.0	37.0	37.0	33.0	37.0
11	35.518578115020546	37.0	37.0	37.0	33.0	37.0
12	35.504040875856745	37.0	37.0	37.0	33.0	37.0
13	35.51618161539708	37.0	37.0	37.0	33.0	37.0
14	35.49958914425707	37.0	37.0	37.0	33.0	37.0
15	35.50366910383878	37.0	37.0	37.0	33.0	37.0
16	35.50055956455011	37.0	37.0	37.0	33.0	37.0
17	35.475621288237704	37.0	37.0	37.0	33.0	37.0
18	35.46396385232072	37.0	37.0	37.0	33.0	37.0
19	35.45738920717234	37.0	37.0	37.0	33.0	37.0
20	35.45637874989276	37.0	37.0	37.0	33.0	37.0
21	35.461018274024575	37.0	37.0	37.0	33.0	37.0
22	35.35134552872654	37.0	37.0	37.0	33.0	37.0
23	35.41465830338503	37.0	37.0	37.0	33.0	37.0
24	35.40482159709446	37.0	37.0	37.0	33.0	37.0
25	35.41745326635082	37.0	37.0	37.0	33.0	37.0
26	35.30915703078082	37.0	37.0	37.0	33.0	37.0
27	35.323465487164334	37.0	37.0	37.0	33.0	37.0
28	35.34452208230461	37.0	37.0	37.0	33.0	37.0
29	35.347750779291346	37.0	37.0	37.0	33.0	37.0
30	35.35215866085812	37.0	37.0	37.0	33.0	37.0
31	35.33979485810701	37.0	37.0	37.0	33.0	37.0
32	35.33500185886009	37.0	37.0	37.0	33.0	37.0
33	35.344293299524324	37.0	37.0	37.0	33.0	37.0
34	35.3173560336692	37.0	37.0	37.0	33.0	37.0
35	35.26078472493637	37.0	37.0	37.0	33.0	37.0
36	35.300856982164476	37.0	37.0	37.0	33.0	37.0
37	35.295476773781495	37.0	37.0	37.0	33.0	37.0
38	35.2870137174342	37.0	37.0	37.0	33.0	37.0
39	35.18343707996912	37.0	37.0	37.0	33.0	37.0
40	34.98357530289887	37.0	37.0	37.0	27.0	37.0
41	35.167734955149044	37.0	37.0	37.0	33.0	37.0
42	35.21009408691839	37.0	37.0	37.0	33.0	37.0
43	34.874609877696535	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	32.0
16	60.0
17	39.0
18	28.0
19	36.0
20	85.0
21	231.0
22	598.0
23	1363.0
24	2794.0
25	4916.0
26	7858.0
27	11639.0
28	16342.0
29	21632.0
30	27783.0
31	35110.0
32	45013.0
33	58812.0
34	84420.0
35	168732.0
36	561504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27794248019599	20.327921985071924	13.418777346691705	24.97535818804038
2	16.353297808451618	20.82495257523617	38.817383678255155	24.004365938057063
3	17.731523407338205	27.041076041676597	29.641287665748354	25.58611288523684
4	12.612604024670409	17.736099062943865	39.415364670219155	30.235932242166573
5	13.920002287827804	37.13649752628619	35.47896628313776	13.464533902748252
6	30.00505228639791	39.552253033754994	17.218859327187975	13.223835352659124
7	27.356128995357615	31.677073105630914	23.485410331449053	17.481387567562415
8	24.76726118414154	35.88896409063611	20.154809681324654	19.188965043897696
9	26.084001410827145	15.655796307064621	20.181119701057167	38.07908258105107
10	15.634252595254663	27.643728015404704	33.515819375994965	23.20620001334566
11	34.08262871414544	23.457861071656673	23.964328951507582	18.495181262690295
12	23.649943280935723	25.711180805124734	29.39067519518031	21.248200718759236
13	27.41065555799167	21.985071923586553	25.98238372591823	24.62188879250355
14	21.95818994690333	20.760702744440103	27.414849908963518	29.86625739969305
15	24.41255254854485	28.09690857268143	24.375089368273546	23.115449510500177
16	23.395327111712724	28.11225608419206	24.73980725050761	23.7526095535876
17	22.66169699627275	27.435821663822768	26.039484094830463	23.862997245074023
18	23.002964643527832	27.307322002230634	27.4755726718969	22.214140682344645
19	25.18917476144629	26.077042601260214	26.71448862282299	22.01929401447051
20	24.099120139557495	26.37446021562777	27.1294433905608	22.396976254253932
21	23.48455239602299	26.89589430235551	27.04317321716252	22.57638008445898
22	23.24509308599373	26.79866162073535	26.806954996520595	23.14929029675033
23	23.018407481196913	26.676930116393237	27.374240965463333	22.930421436946514
24	23.564912347597307	27.40417337921699	25.594120282546733	23.43679399063897
25	23.364060131740754	26.83917523807708	27.012478194141252	22.784286436040915
26	23.412676472550835	27.102847392352935	27.227438681448575	22.257037453647655
27	23.366252633385127	26.94698912328532	25.98581546762247	23.70094277570708
28	22.694298542463038	26.510204665262194	27.6931069654824	23.10238982679237
29	24.587190070827337	26.35501367930374	26.41268600516668	22.64511024470225
30	23.777966311735604	26.212787050894637	27.12296121178613	22.886285425583637
31	23.2761694136488	26.542424906818677	27.068434649152074	23.112971030380447
32	22.227105039893996	26.349580088272024	27.44735612899536	23.97595874283862
33	22.55073734783562	26.64537715794591	27.58939210508756	23.21449338913091
34	23.653661001115317	26.36626216600097	27.35145801359351	22.628618819290203
35	23.616769777794726	26.918105297274625	26.7113428595941	22.75378206533655
36	24.43295234645339	26.75385832626331	26.01317407509795	22.80001525218535
37	23.274262890479775	26.187430292746633	26.671591851519977	23.866714965253617
38	23.835352659123192	26.475219965110625	26.604482235970373	23.084945139795813
39	22.818603853083324	26.69094306168556	27.053563768433698	23.436889316797423
40	23.276741370599506	27.12000610087414	27.261851424649436	22.341401103876915
41	22.830138318255912	25.986292098414726	27.402171529889518	23.781398053439844
42	22.539012230346128	27.296550146325654	26.940411618352194	23.224026004976025
43	23.69932223101341	25.529298494799956	27.05070398368016	23.720675290506467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	38.5
2	52.0
3	158.5
4	265.0
5	265.0
6	300.0
7	335.0
8	351.5
9	368.0
10	479.0
11	590.0
12	590.0
13	963.0
14	1336.0
15	2415.0
16	3494.0
17	4138.0
18	4782.0
19	4782.0
20	4686.0
21	4590.0
22	5254.0
23	5918.0
24	8388.0
25	10858.0
26	10858.0
27	14640.0
28	18422.0
29	22449.5
30	26477.0
31	32984.0
32	39491.0
33	39491.0
34	48771.0
35	58051.0
36	64195.0
37	70339.0
38	74989.0
39	79639.0
40	79639.0
41	83200.5
42	86762.0
43	87988.0
44	89214.0
45	85863.0
46	82512.0
47	82512.0
48	78130.5
49	73749.0
50	74216.0
51	74683.0
52	70376.0
53	66069.0
54	66069.0
55	66138.5
56	66208.0
57	56695.5
58	47183.0
59	43259.5
60	39336.0
61	39336.0
62	35611.0
63	31886.0
64	26061.5
65	20237.0
66	16946.0
67	13655.0
68	13655.0
69	11746.0
70	9837.0
71	8417.0
72	6997.0
73	6849.5
74	6702.0
75	6702.0
76	5724.0
77	4746.0
78	3522.0
79	2298.0
80	1557.5
81	817.0
82	817.0
83	638.5
84	460.0
85	398.5
86	337.0
87	268.5
88	200.0
89	200.0
90	141.5
91	83.0
92	53.5
93	24.0
94	12.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1049030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.711426115860775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78167650153662	46.07456179506955
2	8.537890495702387	9.171645490904574
3	2.0842859668278497	3.358499151347985
4	0.9109263169315538	1.9570860627544933
5	0.5376008426203238	1.4437653969113011
6	0.32137285941888943	1.0356836756592354
7	0.22105065593686218	0.8311062181950734
8	0.17884442055425098	0.7684791104666867
9	0.14498810474297882	0.7008766088023071
>10	1.0112751778838194	10.912955798457542
>50	0.13738698098328356	5.21272117199101
>100	0.11993498450146536	12.423071951210554
>500	0.00881081585392049	3.206742248659184
>1k	0.0039558765058418524	2.9028053195705343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2721	0.2593824771455535	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2052	0.19560927714174048	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1792	0.17082447594444392	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1633	0.15566761675071256	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1600	0.15252185352182493	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1579	0.150520004194351	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1552	0.1479461979161702	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1489	0.14194064993374833	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1469	0.14003412676472551	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1241	0.11829976263786546	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1197	0.11410541166601527	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1166	0.11115030075402992	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1162	0.11076899612022534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1112	0.10600268819766832	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1079	0.10285692496878068	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	1070	0.10199898954272042	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1058	0.10085507564130673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.532615845114058E-5	0.0	0.0
2	0.0	0.0	9.532615845114058E-5	0.0	0.0
3	0.0	0.0	9.532615845114058E-5	1.9065231690228117E-4	0.0
4	0.0	0.0	2.859784753534217E-4	1.9065231690228117E-4	0.0
5	1.9065231690228117E-4	0.0	4.766307922557029E-4	1.9065231690228117E-4	0.0
6	1.9065231690228117E-4	0.0	7.626092676091247E-4	1.9065231690228117E-4	0.0
7	1.9065231690228117E-4	0.0	0.0016205446936693897	2.859784753534217E-4	0.0
8	2.859784753534217E-4	0.0	0.0019065231690228116	2.859784753534217E-4	0.0
9	2.859784753534217E-4	0.0	0.0023831539612785145	7.626092676091247E-4	0.0
10	2.859784753534217E-4	0.0	0.002669132436631936	9.532615845114058E-4	0.0
11	2.859784753534217E-4	0.0	0.004099024813399045	0.0010485877429625464	0.0
12	2.859784753534217E-4	0.0	0.004480329447203607	0.0011439139014136868	0.0
13	2.859784753534217E-4	0.0	0.00495696023945931	0.0011439139014136868	0.0
14	2.859784753534217E-4	0.0	0.005433591031715013	0.001334566218315968	0.0
15	2.859784753534217E-4	0.0	0.005910221823970716	0.0014298923767671087	0.0
16	2.859784753534217E-4	0.0	0.0060055479824218565	0.0015252185352182493	0.0
17	2.859784753534217E-4	0.0	0.006100874140872997	0.0016205446936693897	0.0
18	2.859784753534217E-4	0.0	0.006100874140872997	0.0016205446936693897	0.0
19	2.859784753534217E-4	0.0	0.006196200299324137	0.0016205446936693897	0.0
20	2.859784753534217E-4	0.0	0.0065775049331287	0.0016205446936693897	0.0
21	2.859784753534217E-4	0.0	0.006768157250030981	0.002097175485925093	0.0
22	2.859784753534217E-4	0.0	0.0068634834084821215	0.002097175485925093	0.0
23	2.859784753534217E-4	0.0	0.006958809566933262	0.0023831539612785145	0.0
24	2.859784753534217E-4	0.0	0.006958809566933262	0.0027644585950830766	0.0
25	2.859784753534217E-4	0.0	0.006958809566933262	0.002955110911985358	0.0
26	2.859784753534217E-4	0.0	0.007054135725384403	0.0033364155457899203	0.0
27	2.859784753534217E-4	0.0	0.007054135725384403	0.004099024813399045	0.0
28	3.8130463380456233E-4	0.0	0.007054135725384403	0.010581203588076604	0.0
29	3.8130463380456233E-4	0.0	0.007054135725384403	0.022306321077566894	0.0
30	3.8130463380456233E-4	0.0	0.007054135725384403	0.04127622660934387	0.0
31	3.8130463380456233E-4	0.0	0.007054135725384403	0.10152235875046471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	845	0.0	21.01775	1
GTATCAA	2095	0.0	20.398567	1
GTCGGTA	75	9.264508E-6	19.733334	20
TCGGTAA	80	1.6164513E-5	18.5	21
GATAGCG	60	9.2360814E-4	18.5	25
ACGGTAC	60	9.2360814E-4	18.5	3
CGAGTCG	50	0.007034484	18.499998	21
GTACTAT	195	0.0	18.025642	1
ATATAGC	310	0.0	17.903227	6
TTAACGG	390	0.0	17.551283	35
CGCCGAT	85	2.722749E-5	17.411764	37
GCGTCGG	85	2.722749E-5	17.411764	18
GTACATG	490	0.0	17.367346	1
CGTCCGC	75	2.0670496E-4	17.266666	29
AGTCGCC	65	0.0015798096	17.076923	23
GTCGCCC	435	0.0	17.011494	37
CATGCTA	485	0.0	16.783506	4
AACGGCC	415	0.0	16.493977	37
TATCGCC	90	4.445362E-5	16.444445	33
CATAATA	260	0.0	16.365385	2
>>END_MODULE