FastQCFastQC Report
Fri 10 Feb 2017
ERR1633403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513961
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27500.5350600531946976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20480.39847381416099664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12940.2517700759396141No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11360.22102844379242784No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7370.14339609425617897No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7130.1387264792464798No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6770.13172205673193102No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5570.10837398168343512No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5300.10312066479752355No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5290.10292609750545276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGG401.5977894E-632.37531
CGCCGTT352.3857581E-531.71428725
TACCGGG502.715842E-729.59999832
GGTATCA7250.026.5379311
TTTACCG451.3218218E-424.66666830
TACGGCC551.9000066E-523.54545419
CCGTTTA502.699287E-422.19999927
GACCGTT603.7211288E-521.5833327
GTTACGG603.7211288E-521.58333217
GCCGCTC450.00382297320.55555727
GTATCAA16150.020.5046441
TAAGACT1001.2856617E-820.3499984
ACGGTGT555.137779E-420.18181835
GCGAACT656.892642E-519.92307923
AACCCCG656.892642E-519.92307930
TTATACC701.21753365E-418.54
TATTCCG609.2273473E-418.55
TTTATAC801.613838E-518.53
ACGTATA609.2273473E-418.529
TATACGG609.2273473E-418.532