##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633403.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513961
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.640301501475793	33.0	33.0	33.0	27.0	33.0
2	31.11119520741846	33.0	33.0	33.0	27.0	33.0
3	30.8903418741889	33.0	33.0	33.0	27.0	33.0
4	31.221987660542336	33.0	33.0	33.0	27.0	33.0
5	31.349324170510993	33.0	33.0	33.0	27.0	33.0
6	33.637332404598794	37.0	33.0	37.0	27.0	37.0
7	33.8314774856458	37.0	33.0	37.0	27.0	37.0
8	33.95946385036997	37.0	33.0	37.0	27.0	37.0
9	34.0307241210909	37.0	33.0	37.0	27.0	37.0
10	34.00931393627143	37.0	33.0	37.0	27.0	37.0
11	33.98470895651616	37.0	33.0	37.0	27.0	37.0
12	34.164516373810464	37.0	33.0	37.0	27.0	37.0
13	34.14487675134884	37.0	33.0	37.0	27.0	37.0
14	34.157939999338474	37.0	33.0	37.0	27.0	37.0
15	34.20993421679855	37.0	33.0	37.0	27.0	37.0
16	34.145481855627175	37.0	33.0	37.0	27.0	37.0
17	34.196001253013364	37.0	33.0	37.0	27.0	37.0
18	34.156214187457806	37.0	33.0	37.0	27.0	37.0
19	34.1267489167466	37.0	33.0	37.0	27.0	37.0
20	34.11453009080456	37.0	33.0	37.0	27.0	37.0
21	34.127641980617206	37.0	33.0	37.0	27.0	37.0
22	33.996237068571354	37.0	33.0	37.0	27.0	37.0
23	34.02976295866807	37.0	33.0	37.0	27.0	37.0
24	34.024285889396275	37.0	33.0	37.0	27.0	37.0
25	34.037444475359024	37.0	33.0	37.0	27.0	37.0
26	33.91407130112985	37.0	33.0	37.0	27.0	37.0
27	33.91437482610548	37.0	33.0	37.0	27.0	37.0
28	33.9088335496273	37.0	33.0	37.0	27.0	37.0
29	33.91920204062176	37.0	33.0	37.0	27.0	37.0
30	33.92728242026146	37.0	33.0	37.0	27.0	37.0
31	33.93117571177579	37.0	33.0	37.0	27.0	37.0
32	33.902377806876395	37.0	33.0	37.0	27.0	37.0
33	33.87053297818317	37.0	33.0	37.0	27.0	37.0
34	33.851161858584604	37.0	33.0	37.0	27.0	37.0
35	33.77825554857275	37.0	33.0	37.0	27.0	37.0
36	33.80330997877271	37.0	33.0	37.0	27.0	37.0
37	33.80930459704141	37.0	33.0	37.0	27.0	37.0
38	33.78885946599061	37.0	33.0	37.0	27.0	37.0
39	33.676387897136166	37.0	33.0	37.0	27.0	37.0
40	33.47048900597516	37.0	33.0	37.0	22.0	37.0
41	33.67067734711389	37.0	33.0	37.0	27.0	37.0
42	33.699391588077695	37.0	33.0	37.0	27.0	37.0
43	33.196756174106596	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	21.0
16	35.0
17	35.0
18	44.0
19	78.0
20	221.0
21	554.0
22	1276.0
23	2474.0
24	4173.0
25	6625.0
26	9151.0
27	12432.0
28	16093.0
29	20165.0
30	24909.0
31	30100.0
32	36937.0
33	47027.0
34	64403.0
35	103720.0
36	133483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79752938452528	21.356484246859196	13.55608694044879	23.28989942816673
2	16.886300711532588	23.737209632637494	37.33726878109429	22.039220874735634
3	19.287066528394178	27.73906969594969	30.302882903566612	22.670980872089515
4	13.643642221880647	19.615301550117618	38.18383106889433	28.557225159107404
5	15.152317004597627	36.989187895579626	34.06347952471102	13.79501557511173
6	28.56928833121579	40.354618346528234	17.2933354865447	13.782757835711271
7	25.921227486132214	32.926817404433415	22.773128700426685	18.378826409007686
8	24.56061841268112	35.67780434702244	20.618879642618797	19.142697597677643
9	26.224168759886453	15.361087708989594	20.11008617385366	38.3046573572703
10	15.27353242755773	28.459552378487864	33.07760705578828	23.189308138166126
11	33.68154393037604	24.29639603004897	23.78215467710585	18.239905362469138
12	22.87683306710042	26.381184564587585	30.60854811940984	20.133434248902155
13	29.207079914623872	22.799200717564172	25.922394889884643	22.071324477927316
14	22.157712355606748	21.961004823323172	28.480565646031508	27.400717175038576
15	24.31546362467191	28.67143615955296	24.907726461735425	22.105373754039704
16	22.24157085848926	28.83370528114001	25.264368308101197	23.66035555226953
17	22.32387282303521	27.959514437865906	26.359003893291515	23.357608845807366
18	23.213045347798765	26.47710623957849	28.048431690342262	22.261416722280483
19	25.059683516842718	26.188562945437493	28.075281976648032	20.676471561071754
20	25.094705629415458	25.65428116141108	28.090847360013697	21.16016584915976
21	23.65335112975498	27.060808115790884	27.436712124071672	21.84912863038246
22	22.500734491527567	27.28533877084059	27.80911392109518	22.404812816536662
23	22.74258163557157	26.707668480682383	28.34942729117579	22.200322592570252
24	22.897068065475786	26.841725344919166	27.767087386007887	22.494119203597158
25	23.10467136611533	26.864878852675595	27.976052657691923	22.054397123517152
26	23.111675788629878	26.971112594146245	27.599759514827	22.317452102396874
27	23.180163475438796	26.791916118149047	27.7849875768784	22.24293282953376
28	22.40403454736838	27.42445438467121	27.765141713087182	22.406369354873227
29	23.17296448563218	27.082210517918675	27.296623673780694	22.448201322668453
30	22.89843003652028	27.114508688402427	28.12470206883402	21.862359206243276
31	23.710748480915868	26.59948906629102	27.48282457229245	22.20693788050066
32	21.89563021318738	26.817015298826174	27.97741462873642	23.30993985925002
33	22.147400289126995	26.865267987259735	28.72377476112001	22.263556962493265
34	22.217833648856626	26.609801132770773	27.79646704711058	23.375898171262023
35	22.79161259317341	27.272302762271845	27.763974309334756	22.172110335219987
36	23.042604399944743	26.919941396331627	27.545475240339247	22.491978963384383
37	23.656464206428115	26.61680555528532	27.106920564011666	22.619809674274897
38	23.18327655211193	27.077540902908975	27.284949636256446	22.454232908722645
39	23.058169783310404	26.603380412132438	27.741988205330752	22.5964615992264
40	22.89843003652028	27.257904782658603	27.56785047892739	22.275814701893722
41	23.066341609577382	26.358614758707372	27.990645204597236	22.58439842711801
42	22.663392747698754	26.896009619406918	27.807751950050687	22.632845682843637
43	22.670591737505376	26.803006453797078	27.674473354982187	22.85192845371536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	28.0
2	33.0
3	101.0
4	169.0
5	169.0
6	256.0
7	343.0
8	351.5
9	360.0
10	483.5
11	607.0
12	607.0
13	1001.5
14	1396.0
15	2834.0
16	4272.0
17	4706.5
18	5141.0
19	5141.0
20	4963.5
21	4786.0
22	5227.0
23	5668.0
24	7251.5
25	8835.0
26	8835.0
27	10640.5
28	12446.0
29	15488.5
30	18531.0
31	20099.5
32	21668.0
33	21668.0
34	23743.0
35	25818.0
36	27399.0
37	28980.0
38	30427.5
39	31875.0
40	31875.0
41	33270.5
42	34666.0
43	34976.5
44	35287.0
45	35722.0
46	36157.0
47	36157.0
48	37094.5
49	38032.0
50	38026.5
51	38021.0
52	38863.5
53	39706.0
54	39706.0
55	36804.0
56	33902.0
57	30556.0
58	27210.0
59	23703.0
60	20196.0
61	20196.0
62	17446.5
63	14697.0
64	11878.0
65	9059.0
66	7438.5
67	5818.0
68	5818.0
69	4787.5
70	3757.0
71	3267.0
72	2777.0
73	2150.5
74	1524.0
75	1524.0
76	1168.0
77	812.0
78	638.5
79	465.0
80	373.0
81	281.0
82	281.0
83	257.5
84	234.0
85	214.5
86	195.0
87	155.5
88	116.0
89	116.0
90	91.5
91	67.0
92	46.5
93	26.0
94	15.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	513961.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.047384330166715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.50499447815386	56.43055786333616
2	5.992919714738389	7.556758250298885
3	1.4689406507732945	2.778385973025273
4	0.6708423594758534	1.6917942425131993
5	0.42296591539694917	1.3333447313296116
6	0.2810761355979642	1.063266908824974
7	0.20416570876473708	0.9010479735271885
8	0.1610170179939468	0.8121361453729387
9	0.11813950174345648	0.6703547913894692
>10	0.9888782112464917	12.560803816185913
>50	0.1262328342505104	5.513389349176968
>100	0.05669514349471128	6.521550806805601
>500	0.0018793970219241304	0.7391867689147978
>1k	0.0012529313479494202	1.4274223792990004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2750	0.5350600531946976	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2048	0.39847381416099664	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1294	0.2517700759396141	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1136	0.22102844379242784	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	737	0.14339609425617897	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	713	0.1387264792464798	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	677	0.13172205673193102	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	557	0.10837398168343512	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	530	0.10312066479752355	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	529	0.10292609750545276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	3.891345841415983E-4	0.0	0.0
5	0.0	0.0	7.782691682831966E-4	0.0	0.0
6	0.0	0.0	0.0017511056286371924	0.0	0.0
7	0.0	0.0	0.0021402402127787905	0.0	0.0
8	0.0	0.0	0.002723942088991188	0.0	0.0
9	0.0	0.0	0.0033076439652035853	1.9456729207079914E-4	0.0
10	3.891345841415983E-4	0.0	0.003502211257274385	1.9456729207079914E-4	0.0
11	3.891345841415983E-4	0.0	0.0040859131334867824	3.891345841415983E-4	0.0
12	3.891345841415983E-4	0.0	0.004864182301769979	5.837018762123974E-4	0.0
13	3.891345841415983E-4	0.0	0.005058749593840778	5.837018762123974E-4	0.0
14	3.891345841415983E-4	0.0	0.005253316885911577	5.837018762123974E-4	0.0
15	3.891345841415983E-4	0.0	0.005642451470053175	5.837018762123974E-4	0.0
16	3.891345841415983E-4	0.0	0.0062261533462655725	5.837018762123974E-4	0.0
17	3.891345841415983E-4	0.0	0.006615287930407171	7.782691682831966E-4	0.0
18	3.891345841415983E-4	0.0	0.00680985522247797	7.782691682831966E-4	0.0
19	3.891345841415983E-4	0.0	0.00680985522247797	7.782691682831966E-4	0.0
20	3.891345841415983E-4	0.0	0.00700442251454877	7.782691682831966E-4	0.0
21	3.891345841415983E-4	0.0	0.007782691682831966	0.0011674037524247948	0.0
22	3.891345841415983E-4	0.0	0.007782691682831966	0.0015565383365663931	0.0
23	3.891345841415983E-4	0.0	0.007782691682831966	0.0017511056286371924	0.0
24	3.891345841415983E-4	0.0	0.007782691682831966	0.002723942088991188	0.0
25	3.891345841415983E-4	0.0	0.007782691682831966	0.002918509381061987	0.0
26	3.891345841415983E-4	0.0	0.007782691682831966	0.0033076439652035853	0.0
27	3.891345841415983E-4	0.0	0.007782691682831966	0.004280480425557581	0.0
28	3.891345841415983E-4	0.0	0.007782691682831966	0.010506633771823154	0.0
29	3.891345841415983E-4	0.0	0.007782691682831966	0.021791536711929505	0.0
30	3.891345841415983E-4	0.0	0.007782691682831966	0.04085913133486782	0.0
31	3.891345841415983E-4	0.0	0.007782691682831966	0.08463677205079763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGG	40	1.5977894E-6	32.375	31
CGCCGTT	35	2.3857581E-5	31.714287	25
TACCGGG	50	2.715842E-7	29.599998	32
GGTATCA	725	0.0	26.537931	1
TTTACCG	45	1.3218218E-4	24.666668	30
TACGGCC	55	1.9000066E-5	23.545454	19
CCGTTTA	50	2.699287E-4	22.199999	27
GACCGTT	60	3.7211288E-5	21.583332	7
GTTACGG	60	3.7211288E-5	21.583332	17
GCCGCTC	45	0.003822973	20.555557	27
GTATCAA	1615	0.0	20.504644	1
TAAGACT	100	1.2856617E-8	20.349998	4
ACGGTGT	55	5.137779E-4	20.181818	35
GCGAACT	65	6.892642E-5	19.923079	23
AACCCCG	65	6.892642E-5	19.923079	30
TTATACC	70	1.21753365E-4	18.5	4
TATTCCG	60	9.2273473E-4	18.5	5
TTTATAC	80	1.613838E-5	18.5	3
ACGTATA	60	9.2273473E-4	18.5	29
TATACGG	60	9.2273473E-4	18.5	32
>>END_MODULE