FastQCFastQC Report
Fri 10 Feb 2017
ERR1633402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914119
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163801.7918892398035704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT120591.3191936717210777No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA62510.6838278167284566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61030.6676373645006832No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA52490.5742140793485312No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG44300.4846196173583527No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA43020.4706170640802784No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT40990.44840988974083246No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC34600.3785065182979459No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT32020.3502826218468274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.3431719502603053No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT29870.326762708137562No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC28110.30750919738020976No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26850.29372543399710543No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG26070.2851926280932789No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT25690.2810356200888506No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC24890.27228402429005416No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC24710.27031491523532497No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC24480.26779883144317096No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC23990.26243847901640815No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA22720.2485453206858188No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA21890.23946554004456752No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC21840.2389185653071427No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA21390.23399579267031975No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA21090.23071394424577107No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA21070.23049515435080115No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG19610.21452349201799767No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG19550.21386712233308794No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG19530.213648332438118No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA19410.21233559306829855No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC19150.2094913244336897No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA18950.20730342548399058No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC18900.20675645074656582No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT18820.2058812911666862No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG18690.20445915684938176No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG18580.2032558124270472No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC18000.19691090547291984No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT17790.19461361157573576No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC17410.19045660357130745No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT17280.18903446925400302No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG17260.18881567935903312No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG17240.18859688946406322No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA17190.18804991472663843No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG17130.1873935450417287No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17090.18695596525178887No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG17040.1864089905143641No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG16890.18476806630208978No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT16780.18356472187975528No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.18137682293005614No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA16220.17743860482059776No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT15970.17470373113347387No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT15810.17295341197371458No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG15240.16671789996707212No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG15180.1660615302821624No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC14570.15938843848558013No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT14530.1589508586956403No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC14360.15709114458839604No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG14270.15610659006103145No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14110.15435627090127216No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC13960.1527153466889978No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG13920.152277766899058No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT13560.1483395487895996No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC13340.1459328599449306No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC13140.14374496099523149No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12990.14210403678295713No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA12920.14133827215056244No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG12840.1404631125706828No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA12600.1378376338310439No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG12470.13641549951373946No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA12380.13543094498637487No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG12330.13488397024895007No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC12330.13488397024895007No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG12130.13269607129925098No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG12130.13269607129925098No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA12090.13225849150931115No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT12040.13171151677188636No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG11910.13028938245458194No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG11900.13017998750709697No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA11890.13007059255961204No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG11830.1294142228747023No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG11830.1294142228747023No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG11590.12678874413506339No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC11300.12361629065799967No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT11210.12263173613063508No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11070.1211002068658457No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA11050.12088141697087577No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA10820.11836533317872182No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10730.11738077865135722No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG10530.11519287970165809No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10440.1142083251742935No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC10430.11409893022680853No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA10410.11388014033183863No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA10410.11388014033183863No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA10360.11333316559441386No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG10320.11289558580447404No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA10310.11278619085698907No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC10270.11234861106704927No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10020.10961373737992537No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC9850.10775402327268112No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9800.10720704853525635No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG9630.1053473344280121No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC9580.10480035969058733No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC9440.10326883042579796No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA9420.10305004053082804No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA9290.1016279062135236No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.10151851126603867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAT200.001841738637.02
AGTCCGC200.001841738637.06
TTACAAG200.001841738637.01
TACAAGT200.001841738637.02
TTGGCAA303.598908E-430.83333225
CAAGTCC250.005495761529.64
TAGACCG250.005495761529.65
CCGTCTA250.005495761529.627
ATCGTCA250.005495761529.610
CATACCT250.005495761529.635
TGTCAAC556.259397E-726.909092
GACCGTT2750.026.909097
CGTCTAA358.868003E-426.4285728
GCTTGAT358.868003E-426.4285723
GACCCCC358.868003E-426.4285737
TGGCAAT358.868003E-426.4285726
TCAATTG358.868003E-426.428573
CCATAAT358.868003E-426.428573
GTATTAT901.4188117E-1024.6666681
CGCTCGT451.3229225E-424.66666813
Produced by FastQC (version 0.11.5)