FastQCFastQC Report
Fri 10 Feb 2017
ERR1633401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768056
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24800.32289312237649337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.22537419146520565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10380.13514639557532263No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9010.11730915454081473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCG358.866705E-426.4285724
GTATCAA14650.021.2150171
GGTATCA6000.020.6583331
TAGACTG1303.274181E-1119.9230775
TAGCGTT500.00703280918.526
TGCGGTA500.00703280918.536
CGTTAAG500.00703280918.529
GCGATAA953.603236E-617.52631611
TGGTCGA752.0661413E-417.26666610
GGTTACA650.001579288717.0769231
TAACGGC1851.8189894E-1217.036
TTAACGG1901.8189894E-1216.55263135
GTTACTA904.442867E-516.4444459
CTGGTCG803.3807376E-416.18759
TATAACT1606.2755134E-1016.18754
AACGGCC1951.8189894E-1216.12820637
GTATAAT1504.66207E-916.0333331
CGAACCG2550.015.96078431
GGTCGAT700.002591443715.85714211
AACTGCG700.002591443715.85714210
Produced by FastQC (version 0.11.5)