##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633401.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768056
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362059537325404	33.0	33.0	33.0	27.0	33.0
2	31.59662837084796	33.0	33.0	33.0	27.0	33.0
3	31.343266636807734	33.0	33.0	33.0	27.0	33.0
4	31.654327288635205	33.0	33.0	33.0	27.0	33.0
5	31.75950842126095	33.0	33.0	33.0	27.0	33.0
6	34.72679205682919	37.0	37.0	37.0	27.0	37.0
7	34.8955870405283	37.0	37.0	37.0	27.0	37.0
8	35.00932744487381	37.0	37.0	37.0	33.0	37.0
9	35.15568916849813	37.0	37.0	37.0	33.0	37.0
10	35.16197907444249	37.0	37.0	37.0	33.0	37.0
11	35.152034487068654	37.0	37.0	37.0	33.0	37.0
12	35.17581921109919	37.0	37.0	37.0	33.0	37.0
13	35.1884406866166	37.0	37.0	37.0	33.0	37.0
14	35.17223353505474	37.0	37.0	37.0	33.0	37.0
15	35.185702605018385	37.0	37.0	37.0	33.0	37.0
16	35.15741560511213	37.0	37.0	37.0	33.0	37.0
17	35.15138088889352	37.0	37.0	37.0	33.0	37.0
18	35.125626256418805	37.0	37.0	37.0	33.0	37.0
19	35.11223270178216	37.0	37.0	37.0	33.0	37.0
20	35.120121449477644	37.0	37.0	37.0	33.0	37.0
21	35.11807993167165	37.0	37.0	37.0	33.0	37.0
22	35.0151746747633	37.0	37.0	37.0	27.0	37.0
23	35.069548314185425	37.0	37.0	37.0	27.0	37.0
24	35.047613715666564	37.0	37.0	37.0	27.0	37.0
25	35.0662660014374	37.0	37.0	37.0	33.0	37.0
26	34.940314247919424	37.0	37.0	37.0	27.0	37.0
27	34.9509905527722	37.0	37.0	37.0	27.0	37.0
28	34.97662540231441	37.0	37.0	37.0	27.0	37.0
29	34.9694540502255	37.0	37.0	37.0	27.0	37.0
30	34.96773802951868	37.0	37.0	37.0	27.0	37.0
31	34.97084717780995	37.0	37.0	37.0	27.0	37.0
32	34.958454852250355	37.0	37.0	37.0	27.0	37.0
33	34.95147098649057	37.0	37.0	37.0	27.0	37.0
34	34.93865421271366	37.0	37.0	37.0	27.0	37.0
35	34.86861765287948	37.0	37.0	37.0	27.0	37.0
36	34.89489438270126	37.0	37.0	37.0	27.0	37.0
37	34.89425510639849	37.0	37.0	37.0	27.0	37.0
38	34.883130917537265	37.0	37.0	37.0	27.0	37.0
39	34.78561589259117	37.0	37.0	37.0	27.0	37.0
40	34.55789942400033	37.0	37.0	37.0	27.0	37.0
41	34.74250705677711	37.0	37.0	37.0	27.0	37.0
42	34.78960388305019	37.0	37.0	37.0	27.0	37.0
43	34.41944337392065	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	30.0
16	40.0
17	26.0
18	40.0
19	45.0
20	106.0
21	290.0
22	738.0
23	1534.0
24	2969.0
25	5049.0
26	7642.0
27	11148.0
28	14920.0
29	19435.0
30	24654.0
31	31283.0
32	38667.0
33	50308.0
34	71791.0
35	137852.0
36	349483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.012243898882375	20.790280917016467	13.536903559115482	24.66057162498568
2	16.22824377389149	21.552334725592925	38.44784755278261	23.77157394773298
3	18.117038340954306	27.13265699376087	29.722702511275216	25.027602154009603
4	12.76248086077057	18.20088639370046	39.144801941525095	29.891830804003877
5	14.245966439947086	36.94899851052527	35.37854010645057	13.426494943077067
6	29.77634443321841	39.91935483870967	17.122449404730904	13.181851323341007
7	26.813930234253753	32.04714239586697	23.34478215130147	17.79414521857781
8	24.466705552720114	36.23368608538961	20.339011738727383	18.960596623162896
9	25.88834668305436	15.514363535992167	20.20646931994542	38.390820461008055
10	15.014920787025945	28.17502890414241	33.835163061026805	22.974887247804848
11	34.09334736008833	23.45584176153822	24.077150624433635	18.373660253939818
12	23.015769683460583	25.692788025873114	30.19128813524014	21.100154155426168
13	27.850833793369233	22.39680439967919	25.997453310696095	23.75490849625548
14	21.923141020967222	21.03935129730124	27.668685616673784	29.368822065057753
15	24.021425521055757	28.683064776526713	24.254221046382035	23.041288656035498
16	22.984001166581603	28.415245763329754	25.086842626058512	23.513910444030124
17	22.57843178101597	27.676497547053863	26.31852364931724	23.426547022612933
18	23.041158457195827	27.45711250221338	27.597076254856418	21.904652785734374
19	24.7888174820586	26.46057058339496	27.17080525378358	21.57980668076286
20	24.08639474205006	26.536346308081704	27.365712916766487	22.01154603310175
21	23.152869061630923	26.899340673075923	27.442139635651568	22.50565062964159
22	22.78635932796567	26.980454550189048	27.250226545981022	22.98295957586426
23	22.836355682398157	26.784375097649132	27.66399845844574	22.71527076150697
24	23.156254231462288	27.416230066557645	26.278422406699516	23.14909329528055
25	23.178518233045505	26.858588436259858	27.33785036507755	22.62504296561709
26	23.326033518389284	27.064302602935204	27.30087389461185	22.30878998406366
27	23.22122345245659	26.963268303352883	26.73242576062162	23.083082483568905
28	22.55512618871541	27.014436447342383	27.642385451060857	22.78805191288135
29	23.939400252064953	26.727738602393575	26.707036986886372	22.625824158655096
30	23.5213317778912	26.48257418729884	27.40985032341392	22.586243711396044
31	23.27382378368244	26.716150905663127	27.167680481631546	22.842344829022885
32	22.33782432530961	26.522935827595905	27.66998760507046	23.46925224202402
33	22.424797150207798	26.778125553345067	27.867499244846726	22.929578051600405
34	23.056001124917973	26.60626308498339	27.593040090826708	22.744695699271926
35	23.033216327976085	27.244367598195968	26.902465445227953	22.819950628599997
36	23.794098346995533	26.80390492359932	26.85025571052111	22.551741018884037
37	23.2390606934911	26.454060641411566	27.01521764038039	23.291661024716948
38	23.28905704792359	26.86952513879196	26.83137687876926	23.010040934515192
39	22.829585342735427	26.711463747435083	27.269626173091545	23.189324736737944
40	23.01720187069693	27.36206734925578	27.23317049798452	22.387560282062765
41	22.99064130740467	26.204339266928457	27.453466934702682	23.3515524909642
42	22.45799785432312	27.251268136698364	27.407767141979228	22.882966866999283
43	23.353245075879883	26.261756955222015	27.18070036559834	23.204297603299757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	22.5
2	27.0
3	127.0
4	227.0
5	227.0
6	285.5
7	344.0
8	334.5
9	325.0
10	483.0
11	641.0
12	641.0
13	1006.0
14	1371.0
15	2396.0
16	3421.0
17	4054.0
18	4687.0
19	4687.0
20	4660.0
21	4633.0
22	5230.5
23	5828.0
24	7699.0
25	9570.0
26	9570.0
27	12522.0
28	15474.0
29	18831.5
30	22189.0
31	26137.5
32	30086.0
33	30086.0
34	35869.5
35	41653.0
36	45256.0
37	48859.0
38	52182.5
39	55506.0
40	55506.0
41	57625.0
42	59744.0
43	61175.5
44	62607.0
45	61240.0
46	59873.0
47	59873.0
48	58437.0
49	57001.0
50	55988.0
51	54975.0
52	52865.0
53	50755.0
54	50755.0
55	48548.0
56	46341.0
57	40864.5
58	35388.0
59	32606.0
60	29824.0
61	29824.0
62	26304.0
63	22784.0
64	19175.5
65	15567.0
66	13120.5
67	10674.0
68	10674.0
69	8813.0
70	6952.0
71	5732.0
72	4512.0
73	3580.5
74	2649.0
75	2649.0
76	2020.5
77	1392.0
78	1102.0
79	812.0
80	652.5
81	493.0
82	493.0
83	425.5
84	358.0
85	308.5
86	259.0
87	202.5
88	146.0
89	146.0
90	108.0
91	70.0
92	44.0
93	18.0
94	9.5
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	768056.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.911347912318526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.71169413047429	51.351031272050754
2	8.104191774775977	9.710661059335074
3	2.278754090816799	4.095696874246331
4	1.0334300811480663	2.4765675653886947
5	0.597657983995241	1.790324770585689
6	0.38448875287910245	1.382114366526802
7	0.29813590438207943	1.2503206724811944
8	0.215491601225127	1.032831383454495
9	0.15758507100524713	0.8497020613304502
>10	1.039607373156487	12.031806979990932
>50	0.10742227215268056	4.542780105311247
>100	0.06735856917163252	7.479997035505634
>500	0.003522016688237678	1.3139252170218751
>1k	6.603781290445646E-4	0.6922406367708344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2480	0.32289312237649337	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1731	0.22537419146520565	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1038	0.13514639557532263	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	901	0.11730915454081473	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3019883966794088E-4
2	0.0	0.0	0.0	0.0	1.3019883966794088E-4
3	0.0	0.0	0.0	0.0	1.3019883966794088E-4
4	0.0	0.0	0.0	0.0	1.3019883966794088E-4
5	0.0	0.0	0.0	0.0	1.3019883966794088E-4
6	0.0	0.0	5.207953586717635E-4	0.0	1.3019883966794088E-4
7	0.0	0.0	0.001041590717343527	0.0	1.3019883966794088E-4
8	0.0	0.0	0.0011717895570114679	0.0	1.3019883966794088E-4
9	0.0	0.0	0.0013019883966794087	2.6039767933588176E-4	1.3019883966794088E-4
10	1.3019883966794088E-4	0.0	0.0015623860760152907	2.6039767933588176E-4	1.3019883966794088E-4
11	1.3019883966794088E-4	0.0	0.002864374472694699	2.6039767933588176E-4	1.3019883966794088E-4
12	1.3019883966794088E-4	0.0	0.00299457331236264	2.6039767933588176E-4	1.3019883966794088E-4
13	1.3019883966794088E-4	0.0	0.00299457331236264	2.6039767933588176E-4	1.3019883966794088E-4
14	1.3019883966794088E-4	0.0	0.003254970991698522	2.6039767933588176E-4	2.6039767933588176E-4
15	1.3019883966794088E-4	0.0	0.0036455675107023447	2.6039767933588176E-4	2.6039767933588176E-4
16	1.3019883966794088E-4	0.0	0.004166362869374108	2.6039767933588176E-4	2.6039767933588176E-4
17	1.3019883966794088E-4	0.0	0.00442676054870999	2.6039767933588176E-4	2.6039767933588176E-4
18	1.3019883966794088E-4	0.0	0.00442676054870999	3.9059651900382266E-4	2.6039767933588176E-4
19	1.3019883966794088E-4	0.0	0.004556959388377931	3.9059651900382266E-4	2.6039767933588176E-4
20	1.3019883966794088E-4	0.0	0.004817357067713812	6.509941983397044E-4	2.6039767933588176E-4
21	1.3019883966794088E-4	0.0	0.005207953586717635	9.113918776755862E-4	3.9059651900382266E-4
22	1.3019883966794088E-4	0.0	0.005468351266053517	0.001041590717343527	3.9059651900382266E-4
23	2.6039767933588176E-4	0.0	0.0055985501057214575	0.0013019883966794087	3.9059651900382266E-4
24	2.6039767933588176E-4	0.0	0.0055985501057214575	0.0016925849156832315	3.9059651900382266E-4
25	2.6039767933588176E-4	0.0	0.0055985501057214575	0.0016925849156832315	3.9059651900382266E-4
26	2.6039767933588176E-4	0.0	0.0055985501057214575	0.0023435791140229358	3.9059651900382266E-4
27	2.6039767933588176E-4	0.0	0.0055985501057214575	0.004036164029706167	3.9059651900382266E-4
28	2.6039767933588176E-4	0.0	0.0055985501057214575	0.00885352109741998	3.9059651900382266E-4
29	2.6039767933588176E-4	0.0	0.0055985501057214575	0.018878831751851427	3.9059651900382266E-4
30	2.6039767933588176E-4	0.0	0.0055985501057214575	0.03697647046569521	3.9059651900382266E-4
31	2.6039767933588176E-4	0.0	0.0055985501057214575	0.08684262605851657	3.9059651900382266E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAGCG	35	8.866705E-4	26.42857	24
GTATCAA	1465	0.0	21.215017	1
GGTATCA	600	0.0	20.658333	1
TAGACTG	130	3.274181E-11	19.923077	5
TAGCGTT	50	0.007032809	18.5	26
TGCGGTA	50	0.007032809	18.5	36
CGTTAAG	50	0.007032809	18.5	29
GCGATAA	95	3.603236E-6	17.526316	11
TGGTCGA	75	2.0661413E-4	17.266666	10
GGTTACA	65	0.0015792887	17.076923	1
TAACGGC	185	1.8189894E-12	17.0	36
TTAACGG	190	1.8189894E-12	16.552631	35
GTTACTA	90	4.442867E-5	16.444445	9
CTGGTCG	80	3.3807376E-4	16.1875	9
TATAACT	160	6.2755134E-10	16.1875	4
AACGGCC	195	1.8189894E-12	16.128206	37
GTATAAT	150	4.66207E-9	16.033333	1
CGAACCG	255	0.0	15.960784	31
GGTCGAT	70	0.0025914437	15.857142	11
AACTGCG	70	0.0025914437	15.857142	10
>>END_MODULE