FastQCFastQC Report
Fri 10 Feb 2017
ERR1633400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105094
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40900.37010426262381296No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC35780.3237733622660154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28840.2609732746716569No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20650.18686193210713298No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16480.14912758552666108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16440.14876562536761578No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG16270.14722729469167328No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15560.1408025018686193No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15040.1360970198010305No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13480.12198057359826403No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12510.11320303974141567No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA11820.10695922699788434No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11810.10686873695812302No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11470.10379207560623802No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG11110.10053443417483038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.025.7474231
GACGTAT451.3231671E-424.66666628
TAGCTAG400.001931139923.1250025
GTATCAA24800.022.6028211
TGCGGTA1155.456968E-1222.5217436
GTCTATA806.959617E-720.81251
GGACGTA656.901825E-519.92307527
GTATTAG6200.019.0967731
TTAACGG4650.018.69892535
AACGGCC4750.018.69473637
GTGACAC5150.018.67961324
TATTAGA5950.018.6554622
TCGCAAT500.007034713418.513
CGAGTTC1409.458745E-1118.514
GCGGTAA801.616578E-518.523
ATCAAGC9400.018.530
TTACCGG609.23651E-418.531
GCACCGC5250.018.32380910
TGCATCA9500.018.11052714
GTACATG5150.017.9611661
Produced by FastQC (version 0.11.5)