##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633400.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105094
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833433174010537	33.0	33.0	33.0	27.0	33.0
2	31.953797595498663	33.0	33.0	33.0	33.0	33.0
3	31.66543027109006	33.0	33.0	33.0	27.0	33.0
4	31.956575639719336	33.0	33.0	33.0	33.0	33.0
5	32.05470122903572	33.0	33.0	33.0	33.0	33.0
6	35.22874615191106	37.0	37.0	37.0	33.0	37.0
7	35.38451027695382	37.0	37.0	37.0	33.0	37.0
8	35.53109509236318	37.0	37.0	37.0	33.0	37.0
9	35.64913211002865	37.0	37.0	37.0	33.0	37.0
10	35.64377057517279	37.0	37.0	37.0	33.0	37.0
11	35.679277056974335	37.0	37.0	37.0	33.0	37.0
12	35.66622386873877	37.0	37.0	37.0	33.0	37.0
13	35.670983644830216	37.0	37.0	37.0	33.0	37.0
14	35.646180324931635	37.0	37.0	37.0	33.0	37.0
15	35.66837934148588	37.0	37.0	37.0	33.0	37.0
16	35.644749677403006	37.0	37.0	37.0	33.0	37.0
17	35.64317696051196	37.0	37.0	37.0	33.0	37.0
18	35.62250270112769	37.0	37.0	37.0	33.0	37.0
19	35.619086702126694	37.0	37.0	37.0	33.0	37.0
20	35.62004680144857	37.0	37.0	37.0	33.0	37.0
21	35.6248482029583	37.0	37.0	37.0	33.0	37.0
22	35.52154748826796	37.0	37.0	37.0	33.0	37.0
23	35.58366890056411	37.0	37.0	37.0	33.0	37.0
24	35.57202192754644	37.0	37.0	37.0	33.0	37.0
25	35.59636103354104	37.0	37.0	37.0	33.0	37.0
26	35.488738514551706	37.0	37.0	37.0	33.0	37.0
27	35.49869332382585	37.0	37.0	37.0	33.0	37.0
28	35.52461419571548	37.0	37.0	37.0	33.0	37.0
29	35.535871156661784	37.0	37.0	37.0	33.0	37.0
30	35.53976494307272	37.0	37.0	37.0	33.0	37.0
31	35.527826592127006	37.0	37.0	37.0	33.0	37.0
32	35.5231636403781	37.0	37.0	37.0	33.0	37.0
33	35.52044350978288	37.0	37.0	37.0	33.0	37.0
34	35.50848072652643	37.0	37.0	37.0	33.0	37.0
35	35.44636383873227	37.0	37.0	37.0	33.0	37.0
36	35.47796567531812	37.0	37.0	37.0	33.0	37.0
37	35.476496117072394	37.0	37.0	37.0	33.0	37.0
38	35.46345740724318	37.0	37.0	37.0	33.0	37.0
39	35.37129873114866	37.0	37.0	37.0	33.0	37.0
40	35.17073208251968	37.0	37.0	37.0	33.0	37.0
41	35.34472271137116	37.0	37.0	37.0	33.0	37.0
42	35.393170173758975	37.0	37.0	37.0	33.0	37.0
43	35.07295397495598	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	30.0
16	49.0
17	35.0
18	40.0
19	31.0
20	60.0
21	154.0
22	411.0
23	1031.0
24	2199.0
25	3942.0
26	6628.0
27	10408.0
28	14544.0
29	19965.0
30	26126.0
31	34313.0
32	43683.0
33	58320.0
34	84436.0
35	171952.0
36	626733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.036333560764966	19.537161544628784	13.264753948532885	25.161750946073365
2	16.575241563161143	20.457173778882158	39.1612840174682	23.8063006404885
3	17.896486633716226	26.82839649839742	29.407091161475858	25.868025706410496
4	12.642906395293071	17.188673542703153	39.17558144375049	30.992838618253288
5	14.011206286524041	36.959661350075194	34.9578406904752	14.071291672925563
6	30.385921921574095	39.019033674963396	16.869515172464965	13.725529230997543
7	27.59267537422156	31.445831757298475	23.086633354266695	17.87485951421327
8	24.91326529688877	35.472367056558085	20.062275245363743	19.552092401189398
9	26.647868869073584	15.12269544491238	19.592631939002477	38.63680374701157
10	15.948326567694693	27.241935980106668	33.047686441153424	23.762051011045216
11	34.333640396201595	23.453841935618147	23.48135090770559	18.731166760474675
12	24.186087337366775	25.391686137106888	28.931837472649384	21.49038905287695
13	27.784152298356517	21.70584583754866	25.70496265476059	24.80503920933423
14	22.37999663377052	20.417177181307654	27.303288227064847	29.89953795785698
15	24.846483647544915	27.591137043545615	24.387608655915244	23.174770652994226
16	23.46768691170163	27.54390124279021	24.393128548340684	24.59528329716748
17	22.85715061343198	27.017430191458825	25.590854714621564	24.534564480487635
18	23.417374449594334	26.425082391181203	27.33722199197534	22.820321167249123
19	25.77889301724559	25.273234675059314	26.460825956886925	22.487046350808164
20	24.664779647704176	25.815179523189887	26.839617263327824	22.680423565778117
21	24.252959476750394	26.22328960251345	26.70723033515701	22.816520585579145
22	23.667941369693438	26.40236939120111	26.454672634183158	23.475016604922295
23	23.5381786526757	26.293147913209193	27.01507745042503	23.153595983690074
24	24.011079600468378	26.746412522373664	25.526968746550065	23.715539130607894
25	23.7459437839677	26.200395622453836	26.96304567756227	23.090614916016193
26	24.010084210031003	26.29106664229468	27.159590043923863	22.53925910375045
27	24.04546581557768	26.12347908865671	25.88331852313016	23.94773657263545
28	22.972344434048146	25.881237252215648	27.586974501716593	23.559443812019612
29	24.938964468180988	25.651211571142362	26.287447040704233	23.122376919972417
30	24.109985213927505	25.61329624448237	27.096337506130702	23.180381035459426
31	23.628035262158694	25.80350630806067	27.133709892552126	23.434748537228508
32	22.535277542000955	25.61402016480046	27.35269578877453	24.49800650442406
33	22.93307175679173	26.157955793805776	27.317314183227854	23.59165826617464
34	23.84457792730754	26.004575176410334	27.083397430444833	23.067449465837296
35	23.957419006889914	26.426349251737864	26.59864228744342	23.017589453928807
36	24.739886380706075	26.27305912438218	25.779707427603444	23.2073470673083
37	23.62640644144299	25.914899547006858	26.38961029559476	24.069083715955387
38	24.34299706631291	26.05217293732479	26.2664533514796	23.338376644882697
39	23.002477617288665	26.29170007257301	26.823781506369592	23.882040803768728
40	23.604236381701465	26.388524415117626	27.237501968158366	22.769737235022543
41	23.166717039455467	25.385623304442877	27.29595853384418	24.151701122257474
42	22.679880625539546	26.900336080007676	26.711030916826985	23.708752377625792
43	23.928914644365097	24.989457910367808	27.015982350822643	24.065645094444456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	52.5
2	59.0
3	238.0
4	417.0
5	417.0
6	504.0
7	591.0
8	566.5
9	542.0
10	694.5
11	847.0
12	847.0
13	1411.5
14	1976.0
15	3623.0
16	5270.0
17	5941.5
18	6613.0
19	6613.0
20	6030.0
21	5447.0
22	5776.0
23	6105.0
24	8245.5
25	10386.0
26	10386.0
27	13745.5
28	17105.0
29	21048.5
30	24992.0
31	30757.0
32	36522.0
33	36522.0
34	46070.0
35	55618.0
36	61123.5
37	66629.0
38	71987.0
39	77345.0
40	77345.0
41	81398.5
42	85452.0
43	87590.5
44	89729.0
45	87302.0
46	84875.0
47	84875.0
48	81260.5
49	77646.0
50	79309.5
51	80973.0
52	78125.5
53	75278.0
54	75278.0
55	75744.5
56	76211.0
57	66135.0
58	56059.0
59	50503.5
60	44948.0
61	44948.0
62	41508.5
63	38069.0
64	30382.0
65	22695.0
66	18880.0
67	15065.0
68	15065.0
69	12941.0
70	10817.0
71	9590.5
72	8364.0
73	9195.0
74	10026.0
75	10026.0
76	8719.5
77	7413.0
78	5321.5
79	3230.0
80	1982.5
81	735.0
82	735.0
83	587.0
84	439.0
85	375.0
86	311.0
87	232.0
88	153.0
89	153.0
90	114.0
91	75.0
92	46.5
93	18.0
94	10.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1105094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.12582537712571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.63004523647237	39.882607334722465
2	8.800076609694232	8.294217472275577
3	2.2942446814981325	3.2435452269804115
4	1.032761326174008	1.9467891965410027
5	0.5687688104115338	1.340184981970473
6	0.3734648069920949	1.0559902367286853
7	0.28287560815037405	0.9331522559199933
8	0.1922644666993143	0.724849734711847
9	0.1644551160072066	0.6975074771397514
>10	1.2976432705052228	12.469672787071193
>50	0.17779636627464185	5.858479374083188
>100	0.16645721411195782	15.439171131965118
>500	0.015043668364177289	4.964918788948023
>1k	0.004102818644775625	3.148914000942259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4090	0.37010426262381296	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3578	0.3237733622660154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2884	0.2609732746716569	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2065	0.18686193210713298	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1648	0.14912758552666108	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1644	0.14876562536761578	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1627	0.14722729469167328	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1556	0.1408025018686193	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1504	0.1360970198010305	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1348	0.12198057359826403	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1251	0.11320303974141567	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1182	0.10695922699788434	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1181	0.10686873695812302	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1147	0.10379207560623802	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1111	0.10053443417483038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.8098007952264695E-4	0.0	0.0	0.0	0.0
3	1.8098007952264695E-4	0.0	0.0	0.0	0.0
4	1.8098007952264695E-4	0.0	2.714701192839704E-4	1.8098007952264695E-4	0.0
5	1.8098007952264695E-4	0.0	3.619601590452939E-4	1.8098007952264695E-4	0.0
6	1.8098007952264695E-4	0.0	7.239203180905878E-4	1.8098007952264695E-4	0.0
7	1.8098007952264695E-4	0.0	0.00235274103379441	1.8098007952264695E-4	0.0
8	1.8098007952264695E-4	0.0	0.002895681272362351	1.8098007952264695E-4	0.0
9	1.8098007952264695E-4	0.0	0.0038910717097369093	9.049003976132347E-4	0.0
10	1.8098007952264695E-4	0.0	0.00470548206758882	0.0010858804771358816	0.0
11	1.8098007952264695E-4	0.0	0.0066057729025766135	0.0010858804771358816	0.0
12	1.8098007952264695E-4	0.0	0.0071487131411445545	0.001176370516897205	0.0
13	1.8098007952264695E-4	0.0	0.007691653379712495	0.001176370516897205	0.0
14	1.8098007952264695E-4	0.0	0.007963123498996465	0.0012668605566585286	0.0
15	1.8098007952264695E-4	0.0	0.00913949401589367	0.0012668605566585286	0.0
16	1.8098007952264695E-4	0.0	0.0104063545725522	0.0012668605566585286	0.0
17	1.8098007952264695E-4	0.0	0.010496844612313523	0.0014478406361811756	0.0
18	1.8098007952264695E-4	0.0	0.01094929481112014	0.0014478406361811756	0.0
19	1.8098007952264695E-4	0.0	0.011763705168972051	0.0016288207157038224	0.0
20	1.8098007952264695E-4	0.0	0.012125665328017345	0.0017193107554651459	0.0
21	2.714701192839704E-4	0.0	0.012578115526823962	0.001900290834987793	9.049003976132347E-5
22	2.714701192839704E-4	0.0	0.012668605566585287	0.0026242111530783807	9.049003976132347E-5
23	2.714701192839704E-4	0.0	0.012849585646107933	0.002895681272362351	9.049003976132347E-5
24	2.714701192839704E-4	0.0	0.012940075685869256	0.0033481314711689683	9.049003976132347E-5
25	2.714701192839704E-4	0.0	0.012940075685869256	0.0033481314711689683	9.049003976132347E-5
26	2.714701192839704E-4	0.0	0.012940075685869256	0.003981561749498232	9.049003976132347E-5
27	2.714701192839704E-4	0.0	0.012940075685869256	0.0053389123459180845	9.049003976132347E-5
28	2.714701192839704E-4	0.0	0.012940075685869256	0.013211545805153227	9.049003976132347E-5
29	2.714701192839704E-4	0.0	0.012940075685869256	0.03022367328028204	9.049003976132347E-5
30	2.714701192839704E-4	0.0	0.012940075685869256	0.05456549397607805	9.049003976132347E-5
31	2.714701192839704E-4	0.0	0.012940075685869256	0.11573676085473272	9.049003976132347E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	970	0.0	25.747423	1
GACGTAT	45	1.3231671E-4	24.666666	28
TAGCTAG	40	0.0019311399	23.125002	5
GTATCAA	2480	0.0	22.602821	1
TGCGGTA	115	5.456968E-12	22.52174	36
GTCTATA	80	6.959617E-7	20.8125	1
GGACGTA	65	6.901825E-5	19.923075	27
GTATTAG	620	0.0	19.096773	1
TTAACGG	465	0.0	18.698925	35
AACGGCC	475	0.0	18.694736	37
GTGACAC	515	0.0	18.679613	24
TATTAGA	595	0.0	18.655462	2
TCGCAAT	50	0.0070347134	18.5	13
CGAGTTC	140	9.458745E-11	18.5	14
GCGGTAA	80	1.616578E-5	18.5	23
ATCAAGC	940	0.0	18.5	30
TTACCGG	60	9.23651E-4	18.5	31
GCACCGC	525	0.0	18.323809	10
TGCATCA	950	0.0	18.110527	14
GTACATG	515	0.0	17.961166	1
>>END_MODULE