FastQCFastQC Report
Fri 10 Feb 2017
ERR1633399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129636
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76850.680307638920856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.454394158826383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30020.2657493210202225No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15080.133494329146734No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14610.1293336968722668No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13630.12065833595954803No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA13600.1203927636867097No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13580.12021571550481748No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13190.11676327595791919No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13040.11543541459372753No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG12500.11065511368263758No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG12460.11030101731885317No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC12350.10932725231844595No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG12210.1080879150452004No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11650.10313056595221824No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11570.10242237322464935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.023.8751
GTATCAA42000.023.477381
TACGCAA400.00193116723.12510
CGCTCTC3500.022.72857329
CCGCTCT3500.022.228
GCCGGCA3600.022.09722115
TTCGCCG3750.021.70666524
CCGGTCG555.1430194E-420.1818189
CCGGCAG4050.019.64197516
GCCGCTC4000.019.42500127
TGCGGTA1052.2593667E-819.3809536
TGCTCGC4500.019.32222210
GCTTCGC4300.018.93023322
TTAACGG3350.018.7761235
TCTCGCA500.00703480818.52
AGCTTCG4700.017.71276721
TTGCGGT953.6062756E-617.52631635
CTGCTCG8600.017.4244179
CTTCGCC4750.017.13684323
AGTTCCG650.00157991117.07692332
Produced by FastQC (version 0.11.5)