##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129636
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.832551370529977	33.0	33.0	33.0	27.0	33.0
2	31.945225718727094	33.0	33.0	33.0	33.0	33.0
3	31.672184668335642	33.0	33.0	33.0	27.0	33.0
4	31.955521955745038	33.0	33.0	33.0	33.0	33.0
5	32.05448569273642	33.0	33.0	33.0	33.0	33.0
6	35.210053503960566	37.0	37.0	37.0	33.0	37.0
7	35.358246373167994	37.0	37.0	37.0	33.0	37.0
8	35.5032001458877	37.0	37.0	37.0	33.0	37.0
9	35.625197851343266	37.0	37.0	37.0	33.0	37.0
10	35.628058064721735	37.0	37.0	37.0	33.0	37.0
11	35.64426682577397	37.0	37.0	37.0	33.0	37.0
12	35.6219357385919	37.0	37.0	37.0	33.0	37.0
13	35.641555332868286	37.0	37.0	37.0	33.0	37.0
14	35.616313573575916	37.0	37.0	37.0	33.0	37.0
15	35.651421342804234	37.0	37.0	37.0	33.0	37.0
16	35.63069873835465	37.0	37.0	37.0	33.0	37.0
17	35.61814159605395	37.0	37.0	37.0	33.0	37.0
18	35.60351653098874	37.0	37.0	37.0	33.0	37.0
19	35.59407897765298	37.0	37.0	37.0	33.0	37.0
20	35.588779040328035	37.0	37.0	37.0	33.0	37.0
21	35.59483143242602	37.0	37.0	37.0	33.0	37.0
22	35.49658562581221	37.0	37.0	37.0	33.0	37.0
23	35.54666370406042	37.0	37.0	37.0	33.0	37.0
24	35.539762365930265	37.0	37.0	37.0	33.0	37.0
25	35.55160334833521	37.0	37.0	37.0	33.0	37.0
26	35.44911723776509	37.0	37.0	37.0	33.0	37.0
27	35.46340059983924	37.0	37.0	37.0	33.0	37.0
28	35.477377668558724	37.0	37.0	37.0	33.0	37.0
29	35.48603178368961	37.0	37.0	37.0	33.0	37.0
30	35.488121837476854	37.0	37.0	37.0	33.0	37.0
31	35.48355310914312	37.0	37.0	37.0	33.0	37.0
32	35.47638973970376	37.0	37.0	37.0	33.0	37.0
33	35.471886519197334	37.0	37.0	37.0	33.0	37.0
34	35.44616053312748	37.0	37.0	37.0	33.0	37.0
35	35.38063411576827	37.0	37.0	37.0	33.0	37.0
36	35.40938851098938	37.0	37.0	37.0	33.0	37.0
37	35.39346479750999	37.0	37.0	37.0	33.0	37.0
38	35.385299335361125	37.0	37.0	37.0	33.0	37.0
39	35.2907759667716	37.0	37.0	37.0	33.0	37.0
40	35.07202231515284	37.0	37.0	37.0	33.0	37.0
41	35.23753049654933	37.0	37.0	37.0	33.0	37.0
42	35.26734452513907	37.0	37.0	37.0	33.0	37.0
43	34.9233974483816	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	40.0
16	51.0
17	56.0
18	40.0
19	31.0
20	74.0
21	180.0
22	467.0
23	1146.0
24	2451.0
25	4282.0
26	7199.0
27	10841.0
28	15787.0
29	21340.0
30	28191.0
31	35675.0
32	45742.0
33	60664.0
34	86630.0
35	176166.0
36	632579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85247637292013	19.797173602824273	13.691490002089168	23.658860022166433
2	16.38456989685173	21.64900906132595	38.964232726294135	23.002188315528187
3	18.05856045664267	27.24939715094066	29.64769182285267	25.044350569564
4	12.204196750103574	17.82007655563385	39.57761615245973	30.398110541802847
5	14.347187943727006	36.310457527911645	34.56644441218233	14.775910116179016
6	29.365919641371203	39.80556568664596	16.987241908012845	13.841272763969986
7	27.508684213321814	32.03465541112359	22.38614916663421	18.070511208920394
8	25.03390472683236	34.65310949721857	20.32061655258862	19.992369223360445
9	26.738347573908765	14.868329267126757	19.9285433537883	38.46477980517618
10	15.893172668009873	27.24408570548389	32.86802120329027	23.994720423215973
11	34.26705593660259	23.861314618160186	23.214469085616958	18.657160359620267
12	23.948510847742106	25.57142300705714	29.275182448151437	21.204883697049315
13	28.558048787397	22.41093591209912	25.705359956658608	23.325655343845277
14	22.892329918664064	21.009865124695036	27.588975563809935	28.508829392830965
15	24.485143887057422	27.91846223031136	25.230162636459884	22.366231246171335
16	23.267229443820842	28.03664189172441	24.878190850858154	23.817937813596593
17	23.08903044874632	26.648230049325623	25.457403977918553	24.805335524009504
18	23.68807297217865	25.743956460311107	27.94271783123059	22.625252736279652
19	26.370795548300514	25.29159835557649	27.248688958213087	21.08891713790991
20	25.551859182958047	25.149782761880818	27.392806178273354	21.905551876887777
21	23.421881030703695	26.5166832501797	27.38112099826847	22.680314720848134
22	23.708433513096256	25.771841548959134	27.398294671912016	23.121430266032597
23	22.7276751095043	26.194455559135864	28.320627175479533	22.7572421558803
24	23.307773477474157	26.768534288921387	26.610784358855422	23.312907874749033
25	23.303878417472532	26.500748913809403	28.01167809807761	22.183694570640455
26	24.04765782960175	26.177016313219482	27.1129815267927	22.662344330386073
27	23.65443381761913	26.069105446356172	26.851392838046944	23.425067897977755
28	22.93039527777089	26.05272848953114	27.821528350725366	23.1953478819726
29	24.394760790201445	26.26801907871208	27.025608249028892	22.311611882057584
30	24.08492647189006	25.6873010421056	27.271970794131917	22.955801691872427
31	23.42267774752221	25.558587013869953	27.61933932700445	23.399395911603385
32	22.249910590668147	25.93145048493497	27.804620249354663	24.014018675042227
33	22.7583929690626	25.675084717555034	28.23458175907992	23.331940554302445
34	23.466320124358642	25.75750064622586	27.858265848468	22.917913380947493
35	23.314058687931333	26.60618110612622	26.820940550761485	23.25881965518096
36	23.696128664454744	26.03404990634151	26.905835153978803	23.363986275224942
37	23.595122676685232	25.790785704421605	27.46707789057714	23.147013728316026
38	23.47915611754583	25.896660517193148	26.734009893452402	23.890173471808616
39	22.98395235279329	25.888604824917056	27.569146167438007	23.558296654851652
40	23.47667744299934	26.74118034481904	27.267721637766503	22.514420574415123
41	23.40931060978935	25.587357343427442	27.350226090528277	23.653105956254937
42	22.45643729484542	27.319685279151866	27.07314568586695	23.15073174013576
43	23.84192784224299	25.577708217514317	26.7351607066347	23.845203233607997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	66.0
2	100.0
3	372.0
4	644.0
5	644.0
6	838.5
7	1033.0
8	976.5
9	920.0
10	1232.0
11	1544.0
12	1544.0
13	2550.0
14	3556.0
15	6328.5
16	9101.0
17	10391.0
18	11681.0
19	11681.0
20	10004.5
21	8328.0
22	8347.0
23	8366.0
24	10441.5
25	12517.0
26	12517.0
27	15481.5
28	18446.0
29	22002.0
30	25558.0
31	30585.5
32	35613.0
33	35613.0
34	45533.5
35	55454.0
36	60064.5
37	64675.0
38	68807.5
39	72940.0
40	72940.0
41	75422.0
42	77904.0
43	81624.5
44	85345.0
45	85540.5
46	85736.0
47	85736.0
48	84715.5
49	83695.0
50	85777.5
51	87860.0
52	87195.5
53	86531.0
54	86531.0
55	82970.0
56	79409.0
57	67872.0
58	56335.0
59	52101.0
60	47867.0
61	47867.0
62	42768.5
63	37670.0
64	29627.5
65	21585.0
66	18630.0
67	15675.0
68	15675.0
69	13309.0
70	10943.0
71	10415.5
72	9888.0
73	8788.0
74	7688.0
75	7688.0
76	4899.0
77	2110.0
78	1638.5
79	1167.0
80	946.5
81	726.0
82	726.0
83	580.0
84	434.0
85	359.5
86	285.0
87	231.0
88	177.0
89	177.0
90	126.5
91	76.0
92	48.0
93	20.0
94	11.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1129636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.39492649105444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.96601726527824	40.63529233299749
2	9.240626779323371	8.943989074332475
3	2.4125638591280243	3.5026755185242613
4	1.0849227565295065	2.100190282028705
5	0.6166528750436795	1.492143527911812
6	0.41189985585357397	1.1960317947425783
7	0.30004529832984406	1.0164469109661514
8	0.2226873510614831	0.8621550388086487
9	0.1826736949547397	0.7956432035265536
>10	1.2251206276890247	11.751539632406722
>50	0.16756662033750655	5.63551933027976
>100	0.15233782529963383	14.528972157755563
>500	0.013730814798755509	4.501101535676861
>1k	0.0027832732700180086	1.8872748324253488
>5k	3.7110310266906787E-4	1.1510248276170776
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7685	0.680307638920856	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5133	0.454394158826383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3002	0.2657493210202225	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1508	0.133494329146734	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1461	0.1293336968722668	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1363	0.12065833595954803	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1360	0.1203927636867097	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1358	0.12021571550481748	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1319	0.11676327595791919	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	0.11543541459372753	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1250	0.11065511368263758	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1246	0.11030101731885317	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1235	0.10932725231844595	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1221	0.1080879150452004	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1165	0.10313056595221824	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1157	0.10242237322464935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.852409094611008E-5	0.0	0.0
2	0.0	0.0	8.852409094611008E-5	1.7704818189222016E-4	0.0
3	0.0	0.0	2.655722728383302E-4	1.7704818189222016E-4	0.0
4	0.0	0.0	2.655722728383302E-4	3.540963637844403E-4	0.0
5	0.0	0.0	7.967168185149906E-4	3.540963637844403E-4	0.0
6	0.0	0.0	0.0019475300008144217	3.540963637844403E-4	0.0
7	0.0	0.0	0.004514728638251614	5.311445456766604E-4	0.0
8	8.852409094611008E-5	0.0	0.005488493638658824	6.196686366227705E-4	0.0
9	8.852409094611008E-5	0.0	0.006550782730012145	0.001327861364191651	0.0
10	1.7704818189222016E-4	8.852409094611008E-5	0.007613071821365466	0.001327861364191651	0.0
11	3.540963637844403E-4	8.852409094611008E-5	0.01195075227772486	0.0015934336370299813	0.0
12	3.540963637844403E-4	8.852409094611008E-5	0.01301304136907818	0.0015934336370299813	0.0
13	3.540963637844403E-4	8.852409094611008E-5	0.014252378642323722	0.0016819577279760915	0.0
14	3.540963637844403E-4	8.852409094611008E-5	0.015314667733677043	0.0016819577279760915	0.0
15	3.540963637844403E-4	8.852409094611008E-5	0.016731053188814803	0.0016819577279760915	0.0
16	3.540963637844403E-4	8.852409094611008E-5	0.017793342280168125	0.0017704818189222015	0.0
17	3.540963637844403E-4	8.852409094611008E-5	0.018058914553006455	0.002390150455544972	0.0
18	3.540963637844403E-4	8.852409094611008E-5	0.018590059098683116	0.002390150455544972	0.0
19	3.540963637844403E-4	8.852409094611008E-5	0.019032679553413666	0.002390150455544972	0.0
20	3.540963637844403E-4	8.852409094611008E-5	0.019740872280982546	0.002390150455544972	0.0
21	3.540963637844403E-4	8.852409094611008E-5	0.020537589099497537	0.002567198637437192	0.0
22	3.540963637844403E-4	8.852409094611008E-5	0.020803161372335868	0.0030098190921677426	0.0
23	3.540963637844403E-4	8.852409094611008E-5	0.021068733645174198	0.0038950600016288434	0.0
24	3.540963637844403E-4	8.852409094611008E-5	0.021245781827066417	0.005045873183928274	0.0
25	3.540963637844403E-4	8.852409094611008E-5	0.021245781827066417	0.005488493638658824	8.852409094611008E-5
26	3.540963637844403E-4	8.852409094611008E-5	0.02142283000895864	0.006196686366227705	8.852409094611008E-5
27	3.540963637844403E-4	8.852409094611008E-5	0.021511354099904748	0.007258975457581026	8.852409094611008E-5
28	4.426204547305504E-4	8.852409094611008E-5	0.021511354099904748	0.016819577279760915	8.852409094611008E-5
29	4.426204547305504E-4	8.852409094611008E-5	0.021511354099904748	0.03585225683317458	8.852409094611008E-5
30	4.426204547305504E-4	8.852409094611008E-5	0.021511354099904748	0.062232435935115384	8.852409094611008E-5
31	5.311445456766604E-4	8.852409094611008E-5	0.021511354099904748	0.12579273323442242	8.852409094611008E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1480	0.0	23.875	1
GTATCAA	4200	0.0	23.47738	1
TACGCAA	40	0.001931167	23.125	10
CGCTCTC	350	0.0	22.728573	29
CCGCTCT	350	0.0	22.2	28
GCCGGCA	360	0.0	22.097221	15
TTCGCCG	375	0.0	21.706665	24
CCGGTCG	55	5.1430194E-4	20.181818	9
CCGGCAG	405	0.0	19.641975	16
GCCGCTC	400	0.0	19.425001	27
TGCGGTA	105	2.2593667E-8	19.38095	36
TGCTCGC	450	0.0	19.322222	10
GCTTCGC	430	0.0	18.930233	22
TTAACGG	335	0.0	18.77612	35
TCTCGCA	50	0.007034808	18.5	2
AGCTTCG	470	0.0	17.712767	21
TTGCGGT	95	3.6062756E-6	17.526316	35
CTGCTCG	860	0.0	17.424417	9
CTTCGCC	475	0.0	17.136843	23
AGTTCCG	65	0.001579911	17.076923	32
>>END_MODULE