FastQCFastQC Report
Fri 10 Feb 2017
ERR1633398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242813
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63350.5097307479081727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.35025381935979105No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC26110.21008792151353423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25290.20348998602364152No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC22280.17927073501805985No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG19910.16020109219971146No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA17470.14056821098588443No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17040.13710831798508705No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15900.1279355784015777No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14750.11868237619014285No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13660.1099119497462611No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13310.10709575776886787No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG13300.10701529514094237No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13180.10604974360583612No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG12780.1028312384888153No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12590.1013024485582304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.024.3065681
GTATCAA36450.022.2812061
TAGACCG502.702274E-422.1999995
AGACCGT705.1024745E-621.1428576
CGAGTCG2600.020.63461521
TCGACTA450.003825980220.55555719
AGCTTCG3150.020.55555521
GTATTGC1001.2900273E-820.3499981
CGCCGTC2850.020.12280826
TAGGACC3800.019.9605274
TCCGCCG2550.018.86274531
CCGTCCG2550.018.86274528
TCGCCGT2850.018.82456225
GCTTCGC3450.018.76811622
CTATCGC1103.8524377E-818.532
TATTGCG1205.175025E-918.511
ATCGCTC801.6168433E-518.58
ATACGGT500.00703519718.49999833
TATACCG500.00703519718.4999985
GTAACGG500.00703519718.49999820
Produced by FastQC (version 0.11.5)