##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242813
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818886670802446	33.0	33.0	33.0	27.0	33.0
2	31.931710563053333	33.0	33.0	33.0	33.0	33.0
3	31.65501406889049	33.0	33.0	33.0	27.0	33.0
4	31.9433478729302	33.0	33.0	33.0	33.0	33.0
5	32.04303543654596	33.0	33.0	33.0	33.0	33.0
6	35.186857556205155	37.0	37.0	37.0	33.0	37.0
7	35.34774901775247	37.0	37.0	37.0	33.0	37.0
8	35.48103777478993	37.0	37.0	37.0	33.0	37.0
9	35.607486403827444	37.0	37.0	37.0	33.0	37.0
10	35.60101962242107	37.0	37.0	37.0	33.0	37.0
11	35.63092999509983	37.0	37.0	37.0	33.0	37.0
12	35.61335856641345	37.0	37.0	37.0	33.0	37.0
13	35.628244152579676	37.0	37.0	37.0	33.0	37.0
14	35.60609681424317	37.0	37.0	37.0	33.0	37.0
15	35.62045939332788	37.0	37.0	37.0	33.0	37.0
16	35.60389777062197	37.0	37.0	37.0	33.0	37.0
17	35.59780755431429	37.0	37.0	37.0	33.0	37.0
18	35.58074384481012	37.0	37.0	37.0	33.0	37.0
19	35.56859076948825	37.0	37.0	37.0	33.0	37.0
20	35.57142949100146	37.0	37.0	37.0	33.0	37.0
21	35.57814329267557	37.0	37.0	37.0	33.0	37.0
22	35.47270908817336	37.0	37.0	37.0	33.0	37.0
23	35.52902890458983	37.0	37.0	37.0	33.0	37.0
24	35.52188382323004	37.0	37.0	37.0	33.0	37.0
25	35.547833825362304	37.0	37.0	37.0	33.0	37.0
26	35.43651136574851	37.0	37.0	37.0	33.0	37.0
27	35.44830718700239	37.0	37.0	37.0	33.0	37.0
28	35.46870446318151	37.0	37.0	37.0	33.0	37.0
29	35.47987187131129	37.0	37.0	37.0	33.0	37.0
30	35.478082382466226	37.0	37.0	37.0	33.0	37.0
31	35.46134374197888	37.0	37.0	37.0	33.0	37.0
32	35.46004587979044	37.0	37.0	37.0	33.0	37.0
33	35.45374887452899	37.0	37.0	37.0	33.0	37.0
34	35.43239328845128	37.0	37.0	37.0	33.0	37.0
35	35.36982474435012	37.0	37.0	37.0	33.0	37.0
36	35.39833989506064	37.0	37.0	37.0	33.0	37.0
37	35.38894025086638	37.0	37.0	37.0	33.0	37.0
38	35.3821982872725	37.0	37.0	37.0	33.0	37.0
39	35.27557564975584	37.0	37.0	37.0	33.0	37.0
40	35.069389361070414	37.0	37.0	37.0	33.0	37.0
41	35.23764073919407	37.0	37.0	37.0	33.0	37.0
42	35.27699098738105	37.0	37.0	37.0	33.0	37.0
43	34.95084296672146	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	31.0
16	58.0
17	64.0
18	43.0
19	41.0
20	68.0
21	224.0
22	545.0
23	1402.0
24	2806.0
25	5036.0
26	8238.0
27	12326.0
28	17422.0
29	23756.0
30	30990.0
31	39455.0
32	50296.0
33	66373.0
34	95772.0
35	193597.0
36	694266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.911454096473086	19.74391964036424	13.58193066857202	24.76269559459066
2	16.374225245471365	20.995033041978157	39.09027343614848	23.540468276402002
3	17.887888202006256	27.162573935097235	29.804162009892078	25.14537585300444
4	12.47492583357271	17.60200448498688	39.44688380311439	30.476185878326024
5	14.043544764980734	36.93838091490836	34.84514564942594	14.17292867068497
6	29.777770267932507	39.596705216311705	16.957981611071013	13.667542904684776
7	27.177861834403082	32.04568989864123	22.912055152303683	17.864393114652003
8	24.92683935555872	35.19346836571552	20.214947864240234	19.664744414485526
9	26.69098247282576	15.131479957161698	19.837578139269542	38.339959430743
10	15.958635772236049	27.299682253082324	32.86745471764457	23.87422725703706
11	34.258009853453416	23.613447879930447	23.402394406881807	18.72614785973433
12	24.157375244706966	25.5425393844448	29.04765238213633	21.2524329887119
13	28.023041278132755	22.02825364716977	25.709499337390255	24.23920573730722
14	22.506603970186987	20.67849306371916	27.612762338340524	29.20214062775333
15	24.79174260327177	27.624027106250093	24.842755909376553	22.741474381101582
16	23.105326384580785	27.79726314417374	24.74435011542364	24.353060355821835
17	22.729887762680306	26.842091288069884	25.7317070226977	24.696313926552104
18	23.567262331501198	26.35633840328352	27.521678643528837	22.554720621686446
19	25.847814594794226	25.2288155981632	26.888840074894617	22.03452973214796
20	24.789650574945707	25.423293769859185	27.385053101311303	22.40200255388381
21	24.019703688326402	26.29840531117714	27.112526180527563	22.569364819968893
22	23.454131876637916	26.33260192804549	26.839516483976272	23.373749711340324
23	23.196088228880775	26.26428915693672	27.653717815954614	22.88590479822789
24	23.858858895103285	26.623635253252097	25.986371240081972	23.53113461156264
25	23.538376248075938	26.20885040629604	27.43405484171794	22.81871850391008
26	23.710485809208627	26.428754768416486	27.418927867667943	22.441831554706944
27	23.853548361660202	26.159768203261475	26.363580039796812	23.62310339528151
28	22.76625687050264	25.96440494265831	27.734824144903534	23.534514041935513
29	24.777822568640655	25.790927516850886	26.586381056522583	22.844868857985876
30	24.049716248542623	25.590173260176712	27.411283918015016	22.94882657326565
31	23.444154510775153	25.798812854387588	27.365420220097477	23.391612414739786
32	22.290079038439412	25.749328338213388	27.704489734175613	24.25610288917158
33	22.64274673663697	26.06852358319393	27.87386356595884	23.414866114210263
34	23.51262820713977	25.98749771687293	27.605762089711	22.894111986276293
35	23.68007093585278	26.399868684991223	26.90179455799062	23.01826582116537
36	24.411958999463316	26.189137062454286	26.456031599283236	22.94287233879916
37	23.465638032431265	25.775156841777484	26.8673565532385	23.89184857255275
38	23.922424371164446	26.005199495016544	26.74770862551325	23.324667508305755
39	23.003380234999153	26.17956200973115	27.108663974387138	23.70839378088256
40	23.3513006381491	26.706109446875757	27.34337346004588	22.599216454929262
41	23.04972670868425	25.493296256154384	27.512264516061546	23.944712519099816
42	22.556410336872883	26.906944166177855	27.091123121499372	23.445522375449887
43	23.657300012149857	25.287553316548827	27.11067554008527	23.944471131216037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	62.5
2	84.0
3	325.5
4	567.0
5	567.0
6	689.0
7	811.0
8	791.5
9	772.0
10	1062.0
11	1352.0
12	1352.0
13	2165.0
14	2978.0
15	5355.5
16	7733.0
17	8788.0
18	9843.0
19	9843.0
20	8634.0
21	7425.0
22	7521.0
23	7617.0
24	9927.0
25	12237.0
26	12237.0
27	16164.5
28	20092.0
29	24277.5
30	28463.0
31	35234.5
32	42006.0
33	42006.0
34	52125.0
35	62244.0
36	68288.5
37	74333.0
38	79968.5
39	85604.0
40	85604.0
41	89775.0
42	93946.0
43	96825.5
44	99705.0
45	97884.0
46	96063.0
47	96063.0
48	92678.0
49	89293.0
50	91278.0
51	93263.0
52	89673.5
53	86084.0
54	86084.0
55	85109.0
56	84134.0
57	73092.5
58	62051.0
59	56256.5
60	50462.0
61	50462.0
62	45392.0
63	40322.0
64	32528.0
65	24734.0
66	20844.0
67	16954.0
68	16954.0
69	14392.0
70	11830.0
71	10260.0
72	8690.0
73	9040.0
74	9390.0
75	9390.0
76	8012.5
77	6635.0
78	4819.5
79	3004.0
80	1942.0
81	880.0
82	880.0
83	696.0
84	512.0
85	426.5
86	341.0
87	270.0
88	199.0
89	199.0
90	147.0
91	95.0
92	59.0
93	23.0
94	12.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1242813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.566362048005054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.90308120779927	39.90964337673349
2	9.438904392877625	8.979486873762449
3	2.365821387565916	3.3760054998562214
4	1.0726929993315788	2.040964142902653
5	0.594555168796627	1.4140413208246558
6	0.3953096069107624	1.1282063930023132
7	0.2779861823798938	0.9255953976797354
8	0.2122380484885307	0.8076313483814004
9	0.16742212773209547	0.7167295388296981
>10	1.2316468580010982	11.747583888425433
>50	0.16949634479167058	5.694642800755685
>100	0.15159545907251118	14.628448634063309
>500	0.01409390832776711	4.549208784121263
>1k	0.004984430993966417	3.5638902544284035
>5k	1.718769308264282E-4	0.5179217462333441
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6335	0.5097307479081727	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4353	0.35025381935979105	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2611	0.21008792151353423	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2529	0.20348998602364152	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2228	0.17927073501805985	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1991	0.16020109219971146	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1747	0.14056821098588443	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1704	0.13710831798508705	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1590	0.1279355784015777	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1475	0.11868237619014285	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1366	0.1099119497462611	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1331	0.10709575776886787	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1330	0.10701529514094237	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1318	0.10604974360583612	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1278	0.1028312384888153	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1259	0.1013024485582304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.046262792552057E-5	0.0	0.0
2	0.0	0.0	8.046262792552057E-5	0.0	0.0
3	0.0	0.0	8.046262792552057E-5	8.046262792552057E-5	0.0
4	0.0	0.0	8.046262792552057E-5	3.218505117020823E-4	0.0
5	0.0	0.0	3.218505117020823E-4	3.218505117020823E-4	0.0
6	0.0	0.0	8.046262792552058E-4	3.218505117020823E-4	0.0
7	0.0	0.0	0.003379430372871864	4.023131396276029E-4	0.0
8	8.046262792552057E-5	0.0	0.00426451928005259	4.023131396276029E-4	0.0
9	8.046262792552057E-5	0.0	0.005471458698935399	8.046262792552058E-4	0.0
10	7.241636513296852E-4	0.0	0.005954234466488522	9.655515351062469E-4	0.0
11	7.241636513296852E-4	0.0	0.008931351699732784	9.655515351062469E-4	0.0
12	7.241636513296852E-4	0.0	0.00973597797898799	9.655515351062469E-4	0.0
13	7.241636513296852E-4	0.0	0.010621066886168715	0.0010460141630317674	0.0
14	7.241636513296852E-4	0.0	0.01102338002579632	0.0010460141630317674	0.0
15	7.241636513296852E-4	0.0	0.011908468932977045	0.0011264767909572881	0.0
16	7.241636513296852E-4	0.0	0.012874020468083293	0.0012069394188828086	0.0
17	7.241636513296852E-4	0.0	0.013034945723934334	0.0012069394188828086	0.0
18	7.241636513296852E-4	0.0	0.013598184119412977	0.0012069394188828086	0.0
19	7.241636513296852E-4	0.0	0.01400049725904058	0.0012874020468083291	0.0
20	7.241636513296852E-4	0.0	0.014563735654519223	0.0012874020468083291	0.0
21	7.241636513296852E-4	0.0	0.014885586166221307	0.0014483273026593703	8.046262792552057E-5
22	7.241636513296852E-4	0.0	0.015046511422072348	0.0018506404422869733	8.046262792552057E-5
23	7.241636513296852E-4	8.046262792552057E-5	0.015126974049997868	0.0024138788377656172	8.046262792552057E-5
24	7.241636513296852E-4	8.046262792552057E-5	0.015126974049997868	0.003138042489095302	8.046262792552057E-5
25	8.046262792552058E-4	8.046262792552057E-5	0.015126974049997868	0.003379430372871864	8.046262792552057E-5
26	8.046262792552058E-4	8.046262792552057E-5	0.015207436677923389	0.003942668768350508	8.046262792552057E-5
27	8.046262792552058E-4	8.046262792552057E-5	0.015207436677923389	0.00555192132686092	8.046262792552057E-5
28	8.850889071807263E-4	8.046262792552057E-5	0.015207436677923389	0.014563735654519223	8.046262792552057E-5
29	8.850889071807263E-4	8.046262792552057E-5	0.015207436677923389	0.03355291584494208	8.046262792552057E-5
30	8.850889071807263E-4	1.6092525585104114E-4	0.015207436677923389	0.06050789619999147	8.046262792552057E-5
31	9.655515351062469E-4	1.6092525585104114E-4	0.015207436677923389	0.1279355784015777	8.046262792552057E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1370	0.0	24.306568	1
GTATCAA	3645	0.0	22.281206	1
TAGACCG	50	2.702274E-4	22.199999	5
AGACCGT	70	5.1024745E-6	21.142857	6
CGAGTCG	260	0.0	20.634615	21
TCGACTA	45	0.0038259802	20.555557	19
AGCTTCG	315	0.0	20.555555	21
GTATTGC	100	1.2900273E-8	20.349998	1
CGCCGTC	285	0.0	20.122808	26
TAGGACC	380	0.0	19.960527	4
TCCGCCG	255	0.0	18.862745	31
CCGTCCG	255	0.0	18.862745	28
TCGCCGT	285	0.0	18.824562	25
GCTTCGC	345	0.0	18.768116	22
CTATCGC	110	3.8524377E-8	18.5	32
TATTGCG	120	5.175025E-9	18.5	11
ATCGCTC	80	1.6168433E-5	18.5	8
ATACGGT	50	0.007035197	18.499998	33
TATACCG	50	0.007035197	18.499998	5
GTAACGG	50	0.007035197	18.499998	20
>>END_MODULE