FastQCFastQC Report
Fri 10 Feb 2017
ERR1633397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214670
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90810.7476104621008175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61120.5031819341878865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34680.28550964459482825No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG16910.1392147661504771No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16870.1388854586019248No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16510.13592169066495427No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG15640.12875925148394216No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA15440.12711271374118074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.1229963693842772No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG12700.10455514666534943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG250.005496465629.59999817
GTTGCGC250.005496465629.5999989
GGTTGCG358.869686E-426.4285728
ACAACGC551.9027748E-523.5454543
GGTATCA18250.023.3150671
GTATCAA47950.022.9562051
TACCGTT502.7022252E-422.1999999
TTCGCCG3000.021.58333224
TTGCGGT957.1468094E-921.42105335
TGCTCGC3750.020.72000110
TAAACGC450.003825931820.5555552
TACGCTA450.003825931820.5555559
GCCGGCA3550.020.32394415
TTAACGG2550.020.31372535
GCCGCTC3250.019.92307727
CGCTCTC3250.019.92307729
AGCTTCG3550.019.80281621
CCGCTCT3300.019.62121228
GCTTCGC3600.019.52777722
GCTACCG609.237226E-418.57
Produced by FastQC (version 0.11.5)