##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633397.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1214670
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8729943112121	33.0	33.0	33.0	33.0	33.0
2	31.975959725686813	33.0	33.0	33.0	33.0	33.0
3	31.69379419924753	33.0	33.0	33.0	27.0	33.0
4	31.97535050672199	33.0	33.0	33.0	33.0	33.0
5	32.08043666180938	33.0	33.0	33.0	33.0	33.0
6	35.23144393127352	37.0	37.0	37.0	33.0	37.0
7	35.38736035301769	37.0	37.0	37.0	33.0	37.0
8	35.524605860027826	37.0	37.0	37.0	33.0	37.0
9	35.640962565964415	37.0	37.0	37.0	33.0	37.0
10	35.63578832110779	37.0	37.0	37.0	33.0	37.0
11	35.66316036454346	37.0	37.0	37.0	33.0	37.0
12	35.64014094363078	37.0	37.0	37.0	33.0	37.0
13	35.660040175520926	37.0	37.0	37.0	33.0	37.0
14	35.63677212740909	37.0	37.0	37.0	33.0	37.0
15	35.660250932352	37.0	37.0	37.0	33.0	37.0
16	35.63991866103551	37.0	37.0	37.0	33.0	37.0
17	35.626603933578664	37.0	37.0	37.0	33.0	37.0
18	35.61296401491763	37.0	37.0	37.0	33.0	37.0
19	35.60228951073131	37.0	37.0	37.0	33.0	37.0
20	35.60048984497847	37.0	37.0	37.0	33.0	37.0
21	35.5909588612545	37.0	37.0	37.0	33.0	37.0
22	35.50021816625092	37.0	37.0	37.0	33.0	37.0
23	35.54433220545498	37.0	37.0	37.0	33.0	37.0
24	35.54213901718162	37.0	37.0	37.0	33.0	37.0
25	35.56969547284448	37.0	37.0	37.0	33.0	37.0
26	35.4549721323487	37.0	37.0	37.0	33.0	37.0
27	35.4636106926161	37.0	37.0	37.0	33.0	37.0
28	35.479251977903466	37.0	37.0	37.0	33.0	37.0
29	35.48189631751834	37.0	37.0	37.0	33.0	37.0
30	35.48716112195082	37.0	37.0	37.0	33.0	37.0
31	35.481314266426274	37.0	37.0	37.0	33.0	37.0
32	35.46755579704776	37.0	37.0	37.0	33.0	37.0
33	35.455583821120136	37.0	37.0	37.0	33.0	37.0
34	35.43312916265323	37.0	37.0	37.0	33.0	37.0
35	35.35825038899454	37.0	37.0	37.0	33.0	37.0
36	35.3901965142796	37.0	37.0	37.0	33.0	37.0
37	35.37682580453951	37.0	37.0	37.0	33.0	37.0
38	35.36276931182955	37.0	37.0	37.0	33.0	37.0
39	35.2571727300419	37.0	37.0	37.0	33.0	37.0
40	35.04969580215202	37.0	37.0	37.0	27.0	37.0
41	35.20685700642973	37.0	37.0	37.0	33.0	37.0
42	35.232917582553284	37.0	37.0	37.0	33.0	37.0
43	34.89901701696757	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	35.0
16	54.0
17	54.0
18	46.0
19	36.0
20	61.0
21	187.0
22	479.0
23	1262.0
24	2676.0
25	4650.0
26	7754.0
27	11813.0
28	16843.0
29	23121.0
30	30066.0
31	38452.0
32	49229.0
33	64719.0
34	92369.0
35	187949.0
36	682811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02617171742119	20.058287436093757	13.698453077790676	23.217087768694377
2	16.165460577770094	22.296837824265026	39.44594004956078	22.091761548404094
3	18.093803255205117	27.899264820897855	29.97818337490841	24.028748548988617
4	11.890883943787202	18.062930672528342	40.515201659710044	29.530983723974412
5	13.945186758543473	36.615459342866785	34.98341113224168	14.455942766348063
6	28.488807659693578	40.8202227765566	17.125062774251443	13.565906789498383
7	26.71721537536944	32.54283056303358	22.903257674924056	17.836696386672923
8	24.80879580462183	34.68176541776779	20.637868721545768	19.87157005606461
9	26.727259255600288	14.821062510805405	20.384301909160513	38.0673763244338
10	15.550067096413017	27.72662533856932	33.38091827409914	23.342389290918522
11	33.75739912898153	24.346448006454427	23.598178929256505	18.29797393530753
12	23.47353602212947	26.068397177834306	30.024615739254283	20.43345106078194
13	28.630903866893888	22.920052359900218	25.9697695670429	22.47927420616299
14	22.681880675409783	21.503782920463994	28.33814945623091	27.476186947895314
15	24.071805510961823	28.39125029843497	25.804951138992482	21.731993051610726
16	22.584981929248276	28.558209225550975	25.33898095779101	23.517827887409748
17	22.72205619633316	26.77574979212461	26.01332049033894	24.48887352120329
18	23.522767500638032	25.767080770908972	28.414466480607903	22.295685247845093
19	25.91469287954753	25.617657470753375	27.908979393580147	20.558670256118948
20	25.399161912288935	25.609260128265287	27.82665250644208	21.164925453003697
21	23.244337968337078	26.904097409172863	27.762272880700106	22.08929174178995
22	23.293075485522817	26.1823375896334	28.08301843299003	22.441568491853754
23	22.372907867980604	26.392188824948338	28.955765763540715	22.279137543530343
24	22.581853507537026	27.104398725579788	27.509858644734784	22.8038891221484
25	22.906633077296714	26.866309367976488	28.557138976018177	21.66991857870862
26	23.59710867972371	26.55313788930327	27.625116286728087	22.224637144244937
27	23.196753027571273	26.462742967225665	27.524265849983948	22.81623815521911
28	22.5564968262985	26.295207751899692	28.43455424106959	22.713741180732217
29	23.657865922431608	26.709805955527017	27.61103838902747	22.021289733013905
30	23.518074868071164	26.176410053759458	28.038726567709748	22.26678851045963
31	23.11368519844896	26.049873628228244	28.076432282018988	22.76000889130381
32	21.81473157318449	26.299818057579426	28.420805650917536	23.464644718318556
33	22.236327562218545	26.08148715288926	28.853762750376642	22.828422534515546
34	22.788082359817892	26.075888924563873	28.567759144458986	22.568269571159245
35	22.832785859533868	26.857171083504163	27.624210690969562	22.685832365992407
36	23.132785036264995	26.48176047815456	27.501296648472422	22.88415783710802
37	23.088822478533263	26.18464274247326	27.93408909415726	22.79244568483621
38	22.857236945013874	26.20909382795327	27.46704866342299	23.46662056360987
39	22.59115644578363	26.13878666633736	28.185927041912617	23.084129845966395
40	22.920628648110185	26.891419068553603	28.015839693085365	22.17211259025085
41	23.05622103122659	26.029785867766552	27.926350366766282	22.987642734240577
42	22.065746252068465	27.538508401458834	27.761120304280173	22.63462504219253
43	23.306330114352047	25.829978512682455	27.540648900524424	23.323042472441074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51.0
1	74.5
2	98.0
3	490.5
4	883.0
5	883.0
6	1089.5
7	1296.0
8	1235.0
9	1174.0
10	1577.5
11	1981.0
12	1981.0
13	3127.0
14	4273.0
15	7684.0
16	11095.0
17	12659.0
18	14223.0
19	14223.0
20	12484.0
21	10745.0
22	11128.0
23	11511.0
24	14350.5
25	17190.0
26	17190.0
27	20832.5
28	24475.0
29	29053.5
30	33632.0
31	38848.5
32	44065.0
33	44065.0
34	54335.0
35	64605.0
36	68702.0
37	72799.0
38	75955.0
39	79111.0
40	79111.0
41	80977.0
42	82843.0
43	86172.0
44	89501.0
45	89148.5
46	88796.0
47	88796.0
48	87767.0
49	86738.0
50	88969.0
51	91200.0
52	90631.0
53	90062.0
54	90062.0
55	86280.5
56	82499.0
57	71495.5
58	60492.0
59	54855.5
60	49219.0
61	49219.0
62	43049.0
63	36879.0
64	28639.5
65	20400.0
66	17346.5
67	14293.0
68	14293.0
69	12001.5
70	9710.0
71	9064.0
72	8418.0
73	7293.0
74	6168.0
75	6168.0
76	3884.5
77	1601.0
78	1279.5
79	958.0
80	771.5
81	585.0
82	585.0
83	499.0
84	413.0
85	381.5
86	350.0
87	264.0
88	178.0
89	178.0
90	145.0
91	112.0
92	76.5
93	41.0
94	23.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1214670.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.86869305896109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.36900123382163	46.1908490909891
2	7.721115114266107	8.164105300980804
3	1.6441409274340506	2.6077074611455933
4	0.7400468934945588	1.5650124824560598
5	0.42506503720060734	1.1236316490927394
6	0.29521427144589074	0.9364555622218573
7	0.20345977150342684	0.7529656546622722
8	0.16948376824870948	0.7168308257613694
9	0.12936822528682618	0.6155576094844569
>10	1.0095750974342463	10.864320455839199
>50	0.14598976427154983	5.415623929224562
>100	0.13314961742181827	13.776496797255572
>500	0.011860119371418393	4.243397928878619
>1k	0.0022138889493314336	1.7568516840857296
>5k	3.162698499044905E-4	1.2701935679220753
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9081	0.7476104621008175	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6112	0.5031819341878865	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3468	0.28550964459482825	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1691	0.1392147661504771	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1687	0.1388854586019248	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1651	0.13592169066495427	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1564	0.12875925148394216	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1544	0.12711271374118074	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1494	0.1229963693842772	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1270	0.10455514666534943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.232688713807043E-5	0.0	0.0
2	0.0	0.0	1.6465377427614085E-4	0.0	0.0
3	0.0	0.0	4.939613228284225E-4	0.0	0.0
4	0.0	0.0	6.586150971045634E-4	3.293075485522817E-4	0.0
5	0.0	0.0	9.055957585187746E-4	3.293075485522817E-4	0.0
6	0.0	0.0	0.0018935184041756197	3.293075485522817E-4	0.0
7	0.0	0.0	0.0049396132282842255	5.76288209966493E-4	0.0
8	8.232688713807043E-5	0.0	0.006092189648217211	5.76288209966493E-4	0.0
9	8.232688713807043E-5	0.0	0.007244766068150197	0.0013995570813471973	0.0
10	6.586150971045634E-4	0.0	0.007574073616702479	0.0013995570813471973	0.0
11	6.586150971045634E-4	0.0	0.011525764199329859	0.0014818839684852677	0.0
12	6.586150971045634E-4	0.0	0.012513686844986703	0.0016465377427614084	0.0
13	7.409419842426339E-4	0.0	0.01366626326491969	0.0016465377427614084	0.0
14	7.409419842426339E-4	0.0	0.015065820346266887	0.0016465377427614084	0.0
15	7.409419842426339E-4	0.0	0.01597141610478566	0.0016465377427614084	0.0
16	8.232688713807042E-4	0.0	0.017123992524718648	0.0017288646298994788	0.0
17	8.232688713807042E-4	0.0	0.01745330007327093	0.0018111915170375492	0.0
18	8.232688713807042E-4	0.0	0.018111915170375494	0.0018935184041756197	0.0
19	8.232688713807042E-4	0.0	0.018358895831789703	0.00197584529131369	0.0
20	8.232688713807042E-4	0.0	0.01901751092889427	0.002387479727004042	0.0
21	8.232688713807042E-4	0.0	0.020334741123103395	0.0027991141626943945	0.0
22	8.232688713807042E-4	0.0	0.02091102933306989	0.0032107485983847465	0.0
23	8.232688713807042E-4	0.0	0.020993356220207957	0.00386936369548931	0.0
24	8.232688713807042E-4	0.0	0.021158009994484098	0.004857286341146155	0.0
25	8.232688713807042E-4	0.0	0.021322663768760238	0.0053512476639745775	0.0
26	8.232688713807042E-4	0.0	0.021322663768760238	0.006503824083907563	0.0
27	9.055957585187746E-4	0.0	0.021322663768760238	0.00798570805239283	0.0
28	9.055957585187746E-4	0.0	0.02140499065589831	0.018688203380341988	0.0
29	9.055957585187746E-4	0.0	0.02156964443017445	0.043633250183177326	0.0
30	9.055957585187746E-4	0.0	0.02156964443017445	0.0768109856998197	0.0
31	9.87922645656845E-4	0.0	0.02156964443017445	0.15543316291667697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCG	25	0.0054964656	29.599998	17
GTTGCGC	25	0.0054964656	29.599998	9
GGTTGCG	35	8.869686E-4	26.428572	8
ACAACGC	55	1.9027748E-5	23.545454	3
GGTATCA	1825	0.0	23.315067	1
GTATCAA	4795	0.0	22.956205	1
TACCGTT	50	2.7022252E-4	22.199999	9
TTCGCCG	300	0.0	21.583332	24
TTGCGGT	95	7.1468094E-9	21.421053	35
TGCTCGC	375	0.0	20.720001	10
TAAACGC	45	0.0038259318	20.555555	2
TACGCTA	45	0.0038259318	20.555555	9
GCCGGCA	355	0.0	20.323944	15
TTAACGG	255	0.0	20.313725	35
GCCGCTC	325	0.0	19.923077	27
CGCTCTC	325	0.0	19.923077	29
AGCTTCG	355	0.0	19.802816	21
CCGCTCT	330	0.0	19.621212	28
GCTTCGC	360	0.0	19.527777	22
GCTACCG	60	9.237226E-4	18.5	7
>>END_MODULE