FastQCFastQC Report
Fri 10 Feb 2017
ERR1633396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961462
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76060.7910869072308631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.5470835040802445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29740.30932059717388727No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14770.15362021587956673No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13410.13947509105924102No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.12990633015137362No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA11680.12148166022162082No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10000.10400827073768906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.024.8861221
GTATCAA40100.023.943891
GCCGGCA2300.020.91304415
TTCGCCG2250.020.55555524
CCGGCAG2500.019.9816
ATGCGGA759.263185E-619.7333346
CCTACCG1153.0486262E-919.30434824
GCCGCTC2350.018.89361827
CGCTCTC2600.018.50000229
GCGACAA1103.849709E-818.516
GCGAACT2100.018.528
CCGCTCT2500.018.528
TCGATCA801.6162223E-518.534
TTCGATC701.2189339E-418.533
GACTCGG609.235321E-418.533
CCGCCCA500.00703406718.4999989
GCTTTAT4450.017.8764061
GCTTCGC2800.017.83928722
CTACCGA1156.4015694E-817.69565225
AGCTTCG2850.017.52631621
Produced by FastQC (version 0.11.5)